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Application of a kind of Bacillus subtilis in biological control of Phellodendron blight

A technology of Bacillus subtilis and biological control preparation, which is applied in the field of microorganisms and achieves the effect of good application prospects

Active Publication Date: 2021-07-23
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there are few reported strains that have inhibitory effect on Phellodendron twig blight, so there is an urgent need to screen strains that can be used for biological control of Phellodendron twig blight

Method used

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  • Application of a kind of Bacillus subtilis in biological control of Phellodendron blight
  • Application of a kind of Bacillus subtilis in biological control of Phellodendron blight

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Example 1 Screening of Phellodendron Phellodendron Branch Blight Biocontrol Bacteria

[0013] 1. Take the sample of Phellodendron twig blight, select the late diseased branch, scrape off the dense gray-black small spots on the branch, grind it, shake it in water for 10 minutes, make a suspension, and then add 1ml of the suspension to 9ml of sterile water , and then serially diluted to 10 -7 times. The bacterial suspension obtained above was evenly spread on NA and PDA plates, set up 2 repetitions, cultured at a constant temperature of 28°C for 3-7 days, picked a single colony with different traits, and moved the bacteria to the slant of the NA test tube for storage until screening.

[0014] 2. Place commercially available Bacillus subtilis ATCC NO.6051, ATCC NO.11744, CGMCC NO.1.1080, and CGMCC NO.1.406 in beef extract peptone medium, activate at 30°C for 30 hours, and use plate colony confrontation method to determine Inhibitory activities of the above four Bacillus ...

Embodiment 2

[0020] Embodiment 2 control effect test

[0021] The Bacillus subtilis strain ATCC NO.6051 was cultivated according to the conventional methods in the field for seed cultivation and small-scale fermentation to obtain the fermentation liquid of the Bacillus subtilis strain ATCC No.6051, and the concentration of the fermentation liquid bacteria was adjusted to 1×10 9 / ml bacterial solution, used for 1-year-old Phellodendron garden experiment.

[0022] This experiment was carried out in a 1-year-old Cortex Phellodendron garden, and the subjects were all in the early stage of the onset of Cortex Phellodendri Blight. There were 3 experimental groups, namely the 10-fold fermentation broth group of Bacillus subtilis strain ATCC NO.6051, the 50 % Carbendazim wettable powder 800 times liquid, clear water group (control group), each group was treated with 50 Phellodendron cork, sprayed once every 30 days. The disease incidence survey was carried out 15 days after the last spraying, and...

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Abstract

The invention provides a Bacillus subtilis used for inhibiting Phellodendron twig blight, the preservation number is ATCC NO.6051, and the bacterial strain has good control effect on Phellodendron twig blight.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a bacillus subtilis and its application in biological control of Cortex Phellodendron blight. Background technique [0002] Cortex Phellodendron (alias: Phellodendron cortex) is a traditional Chinese medicine commonly used in Chinese medicinal materials. It is used as medicine after the inner layer of the bark is processed. Bitter in the mouth, cold in nature. Heat-clearing and toxic substances removing, purging fire and drying damp. Indications for acute bacillary dysentery, acute enteritis, acute icteric hepatitis, urinary system infection and other inflammations. External use to treat burns, otitis media, acute conjunctivitis, etc. Cortex Phellodendron is a deciduous tree of the Rutaceae Phellodendron genus, the bark is grayish brown to black gray, the cork layer is developed, soft, and the inner bark is bright yellow; the branchlets are orange yellow or light yellow gray,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCA01N63/22C12N1/20
Inventor 张娜赵良友刘永武安柏松
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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