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Production method for organic acid fermentation aspergillus niger mycelium extract

A technology of Aspergillus niger and a production method, applied in the field of microorganisms, can solve the problems of inability to obtain high-value utilization, low added value, etc., and achieve the effects of good quality, increased added value, and less product impurities

Inactive Publication Date: 2016-01-13
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As a fermentation production strain, Aspergillus niger is widely used in the production of bulk fermentation products such as citric acid, gluconic acid, and itaconic acid. At present, the treatment method for Aspergillus niger mycelium in the industry is to produce low-protein feed by drying. The added value is low; there are also chemical methods to produce chitosan or glucosamine hydrochloride using Aspergillus niger mycelia as raw materials. This treatment method mainly uses the cell wall of Aspergillus niger, and the bacteria contained in Aspergillus niger cells Nutrients such as protein and nucleic acid cannot be utilized with high value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) 500Kg of the citric acid fermented Aspergillus niger mycelia after filtration and washing were used in a 1.25m 3 The warm water is used for pulping, the water temperature is 50°C, and the pH after pulping is 4.2;

[0025] (2) Add 800g of compound wall-breaking enzyme (the mass ratio of helicase, cellulase and pectinase is 2.5:1.5:1.0) to the slurry mixing liquid, and stir and react at 50°C for 4h;

[0026] (3) Add 1650 g of acid protease and 550 g of nuclease to the feed liquid after reacting for 4 h, and stir and react for 3.5 h at 50° C.;

[0027] (4) Put the obtained hydrolyzate into a centrifuge for centrifugal separation, the centrifugal speed is 650r / min, the time is 10 minutes, and the liquid phase after centrifugation is 1.29m 3 ;

[0028] (5) 1.29m will be obtained 3 The liquid phase was evaporated and concentrated to 305 L under reduced pressure at 70°C, and dried at 105°C to obtain 122Kg of Aspergillus niger mycelia extract with a product yield of 24.4...

Embodiment 2

[0030] (1) 500Kg of the citric acid fermented Aspergillus niger mycelium after filtering and washing was used in a 2.0m 3 The warm water is used for pulping, the water temperature is 52°C, and the pH after pulping is 4.7;

[0031] (2) Add 1040g of compound wall-breaking enzyme (the mass ratio of helicase, cellulase and pectinase is 3.0:1.5:1.0) to the slurry mixing liquid, and stir and react at 52°C for 4h;

[0032] (3) Add 1760g of acid protease and 568g of nuclease to the feed liquid after reacting for 4h, and stir and react for 3.5h at 52°C;

[0033] (4) Put the obtained hydrolyzate into a centrifuge for centrifugal separation, the centrifugal speed is 800r / min, the time is 5 minutes, and the liquid phase after centrifugation is 1.47m 3 ;

[0034] (5) 1.47m will be obtained 3 The liquid phase was evaporated and concentrated to 319L under reduced pressure at 70°C, and dried at 105°C to obtain 127.6Kg of Aspergillus niger mycelia extract with a product yield of 25.5%.

Embodiment 3

[0036] (1) 500Kg of the citric acid fermented Aspergillus niger mycelia after filtration and washing were used in a 1.0m 3 Mix with warm water, the water temperature is 45°C, and the pH after mixing is 4.1;

[0037] (2) Add 950g of compound wall-breaking enzyme (the mass ratio of helicase, cellulase and pectinase is 4.0:1.5:0.8) to the slurry mixing liquid, and stir and react at 45°C for 3.5h;

[0038] (3) Add 1980g of acid protease and 670g of nuclease to the feed liquid after reacting for 3.5h, and stir and react for 3.5h at 45°C;

[0039] (4) Put the obtained hydrolyzate into a centrifuge for centrifugal separation, the centrifugal speed is 800r / min, the time is 15 minutes, and the liquid phase after centrifugation is 1.03m 3 ;

[0040] (5) 1.03m will be obtained 3 The liquid phase was evaporated and concentrated to 302L under reduced pressure at 70°C, and dried at 105°C to obtain 120.8Kg of Aspergillus niger mycelia extract with a product yield of 24.2%.

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a production method for organic acid fermentation aspergillus niger mycelium extract. The method includes the steps that an organic acid fermentation aspergillus niger mycelium obtained after starch raw materials are adopted for producing organic acid through aspergillus niger fermentation is filtered, washed and subjected to serous mixing, and mycelium serous fluid is obtained; a cell wall-breaking enzyme is added into the mycelium serous fluid for carrying out a cell wall-breaking reaction, and wall-broken serous fluid is obtained; protease and nuclease are added into the serous fluid for carrying out a hydrolysis reaction, so that hydrolysate is obtained; the hydrolysate is subjected to centrifugal separation, so that a liquid phase is obtained; the liquid phase is directly subjected to evaporation and concentration, so that the aspergillus niger mycelium extract is prepared. According to the method, pollution is avoided, the reaction condition is moderate, product quality is good, and high-valued utilization of the aspergillus niger mycelium is realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a production method of an organic acid fermentation Aspergillus niger mycelia extract. Background technique [0002] As a fermentation production strain, Aspergillus niger is widely used in the production of bulk fermentation products such as citric acid, gluconic acid, and itaconic acid. At present, the treatment method for Aspergillus niger mycelia in the industry is to produce low-protein feed by drying. The added value is low; there are also chemical methods to produce chitosan or glucosamine hydrochloride using Aspergillus niger mycelia as raw materials. This treatment method mainly uses the cell wall of Aspergillus niger, and the bacteria contained in Aspergillus niger cells Nutrients such as protein and nucleic acid cannot be utilized with high value. If the nutrients contained in Aspergillus niger mycelium cells such as bacterial protein and nucleic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12P13/04C12P19/30
Inventor 高秀珍马钦元朱海亮
Owner SHANDONG UNIV OF TECH
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