615 results about "Inducible promoters" patented technology

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

Described herein is a composition useful for inducing expression of genes whose expression is under control of an inducible promoter sequence and methods for the compositions preparation and use.

A method of treating brain disorders involving loss of cells that respond to GDNF is disclosed. In one embodiment, the invention comprises the steps of (a) transducing human neural stem cells with glial-derived neurotrophic factor (GDNF), wherein the GDNF gene is under control of an inducible promoter system, and (b) transplanting the transduced cells into the brain of a patient.

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

The invention provides a novel fusion protein preparation method and an application of the novel fusion protein for increasing protein synthesis. The provided fusion protein can greatly increase the in-vitro translation efficiency. In addition, a composition-type or induction-type promoter (such as pK1PGK1) is inserted before eIF4G, and the synthesis capability of the in-vitro protein is obviouslyenhanced.

This invention relates to an isolated isoflavone synthase 1 (IFS1) promoter nucleic acid fragment. The invention also relates to the construction of chimeric genes comprising all or a portion of the IFS1 promoter directing the expression of transgenes, in sense or antisense orientation.

Disease-inducible promoter sequences have been identified that may be used to produce transgenic plants that are both more resistant to disease than control plants, and are wild-type or nearly wild type in appearance. Any of these disease-inducible promoters may be incorporated into expression vectors that each comprise a defense response protein operably linked to the promoter. The expression vectors can be introduced into plants and the defense response protein then ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, in some cases, to more than one type of pathogen, and yet are similar to wild type plants in their morphology and development.

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

The invention belongs to the technical field of bioengineering, and particularly relates to a genetic engineering strain capable of effectively secreting D-psicose 3-epimerase and a construction method thereof. A D-psicose 3-epimerase gene rdpe from rumen bacterium Ruminococcus sp. 5_1_39B_FAA is obtained firstly, recombinant plasmid pMA5-RDPE construction and bacillus subtilis conversion are conducted, and then constitutive and secretive expression of RDPE in bacillus subtilis is achieved. By comparing three glucose-induced promoters, the optimal inducible promoter P[xylA] is obtained, and the RDPE secretion level is increased remarkably. By knocking out the xylose utilization gene xylAB (xylA and xylB), the xylose metabolism pathway of bacillus subtilis is blocked, the secretion amount of RDPE is further increased, and the optimal induced concentration of the inducer xylose is reduced to 0.5% from 4.0%. Finally, the engineered strain 1A751SD-XR is evaluated in a 7.5 L fermentation tank, and the RDPE secretion level can be as high as 95 U / mL and 2.6 g / L.

The present invention is directed to drought responsive promoter sequences, polynucleotide constructs comprising the drought responsive promoters and methods of identifying the drought responsive promoters or fragments thereof. The invention further relates to the use of the present drought responsive promoters to modulate transcript levels.

The invention provides an attenuated Salmonella vaccine vector comprising one or more heterologous polynucleotides that encode immunogenic Chlamydial peptides. In one embodiment, the attenuated Salmonella vaccine vector comprises aroC and ssaV attenuating mutations. The heterologous polynucleotides encoding the immunogenic Chlamydial peptides can be under the control of an inducible promoter such as a Salmonella ssaG promoter. In one embodiment of the invention, the immunogenic Chlamydial peptide is a PmpG peptide, for instance, a CT110, CT84 or CT40 peptide.

The invention relates to a maltose inducible trehalose synthase synthesis engineering bacterium, a method for preparing the same and application. The maltose inducible trehalose synthase synthesis engineering bacterium is characterized in that maltose inducible promoters are inserted in the fronts of BamHI cleavage sites of PHT01 plasmids of recombinant plasmid vectors instead of Pgrac promoters on the PHT01 plasmids, expression genes of Tat type signal peptides are inserted in the fronts of the BamHI cleavage sites, and expression genes of trehalose synthase are inserted in the rears of the BamHI cleavage sites. The maltose inducible trehalose synthase synthesis engineering bacterium, the method and the application have the advantage that expression effects realized after the maltose inducible promoters and the trehalose synthase are fused with one another are obviously superior to other inducible expression effects.

The invention provides compositions and methods for gene therapy using cytolethal distending toxins (CDTs). In a preferred embodiment, a gene therapy vector according to the invention includes a gene encoding a B subunit of a CDT and an antisense oligonucleotide that inhibits a DNA repair mechanism. An inducible promoter is operably linked to the gene and oligonucleotide. Preferably, the promoter is strictly inducible by heat shock.

A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected protein that modifies sensitivity to ethylene in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, modulates expression of the selected protein and selectively modulates ethylene sensitivity in the plant cell. This modulation in the expression of the protein is controlled by the timing, the concentration and the duration of the application of the inducing composition. Transgenic plant cells, tissues, organs and entire plants are provided, which in the presence of the inducing composition control ethylene sensitivity. Ethylene sensitivity and / or ethylene production in such transgenic plants and tissues may be controlled for purposes of manipulating ripening, flower senescence and other ethylene sensitive functions of the plant.

The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and / or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.