166 results about "Enzyme synthesis" patented technology

The invention relates to the technical field of biology, and discloses a coenzyme-Q10-production engineered bacteria construction method, engineered bacteria, and an application thereof. The method comprises the steps that: a, total genomic DNA is extracted from Rhodobacter sphaeroides cacterial liquid; b, UbiG gene is obtained by amplification with a polymerase chain reaction; c, the amplified UbiG gene is connected with broad-host plasmid, such that recombinant vector is constructed; d, the recombinant vector is transferred to Escherichia coli S17-1; and e, the Escherichia coli S17-1 is subjected to conjugal transfer with the Rhodobacter sphaeroides, such that the engineered bacteria are obtained. According to the method provided by the invention for improving coenzyme Q10 yield through regulating Rhodobacter sphaeroides aromatic ring modification pathway, the operation is simple, and coenzyme Q10 synthesis capacity can be improved by higher than 30%. The method is suitable for coenzyme Q10 large-scale industrial production.

A method for producing lacto-N-biose I and galacto-N-biose inexpensively and conveniently is provided. The method for producing lacto-N-biose I or galacto-N-biose, characterized in that the method comprises causing: (i) a combination of a carbohydrate raw material with an enzyme that catalyzes phosphorolysis of the carbohydrate raw material to give a-glucose-1-phosphate; and (ii) a combination of an enzyme that converts α-glucose-1-phosphate to UDP-glucose and an enzyme that converts UDP-galactose to galactose-1-phosphate with their cofactors, and/or a combination of an enzyme (UDP-Gly synthase) that converts α-glucose-1-phosphate and UDP-galactose to UDP-glucose and α-galactose-1-phosphate, respectively, with its cofactor(s) to act in the presence of N-acetylglucosamine or N-acetylgalactosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211), and UDP-glucose-4-epimerase (EC 5.1.3.2).

The invention discloses acyl-coenzyme A synthetase and an application thereof. The amino acid sequence of the acyl-coenzyme A synthetase is as shown in SEQ ID No.1. Further, genetically engineered bacteria capable of secreting the acyl-coenzyme A synthetase can be established, and the acyl-coenzyme A synthetase can be produced by fermentation of recombinant bacteria. The acyl-coenzyme A synthetase disclosed by the invention is the first enzyme which has been ever found to have an activity on trans-8-methyl-6-nonenoic acid or 8-metyl nonanoic acid, and provides a direct substrate for cloning capsaicinoid synthetase. The acyl-coenzyme A synthetase disclosed by the invention has an activity on medium-chain fatty acid and can also be applied to the field of biodiesel.

The invention provides novel flavone hydroxylase, a microorganism for synthesizing a flavone C-glycoside compound and an application of the novel flavone hydroxylase. The inventor clones to obtain novel flavone hydroxylase PhF2H and PhF3'H, and the novel flavone hydroxylase PhF2H and PhF3'H belongs to cytochrome P450 hydroxylase and has a function of hydroxylating a specific position of the compound. Furthermore, the inventor efficiently synthesizes the flavone C-glycoside compound, such as orientin, isoorientin, vitexin and isovitexin, and related intermediates such as eriodictyol, 2-hydroxynaringenin and the like in a synthetic route of the flavone C-glycoside compound in an artificial recombinant expression system by modifying the enzyme and combining with the assembly of a synthetic route of C-glycoside glycosyltransferase and a flavone precursor.

The invention relates to Issatchenkia orientalis and a method for producing citicoline by whole cell conversion of Issatchenkia orientalis, belonging to the technical field of biological pharmacy. In the method provided by the invention, the whole cells of Issatchenkia orientalis Z1, namely CCTCC (China Center for Type Culture Collection) NO: M2011272 are utilized to prepare citicoline, choline phosphate and 5'-cytidylic acid are used as substrates, glucose is used as an energy donor, and the ATP (adenosine triphosphate) regeneration efficiency is improved by adding inorganic ions; glucose is used as the energy donor, so that the energy requirement of the strain is provided and ATP is provided for a citicoline synthesis enzyme system; one or more of potassium ion, magnesium ion and manganese ion are added to change the metabolism flow direction and improve the ATP regeneration rate, so that the ATP regeneration rate is matched with the rate of the citicoline enzyme synthesis system and the high-efficiency preparation of citicoline is achieved; and the whole cells of Issatchenkia orientalis are used, and toluene is added to an aqueous solution during the preparation process so as to improve the cell permeability, so that the rate of the citicoline enzyme synthesis system is improved.

An analytical system for rapid detection and identification of different analytes directly from a test sample by mixing test material with a germinogenic source and enzyme-free spores, allowing the mixture to stand for a time to allow analyte-induced spore germination and subsequent de novo activity of an enzyme capable of producing a germinant in the presence of the germinogenic source and detecting the presence of a germination-derived product. The germinant which is formed promotes further spore germination with concomitant additional de novo enzyme synthesis or activation which results in a propagating cascade of analyte-independent germination after which a germination-derived product can be easily detected. The technique is particularly efficient to conduct thousands of parallel assays in an array of microscopic wells.

The invention discloses a preparation method of eco-friendly efficient compound foliar fertilizer. According to the characteristics of crop growth and development, various nutrients such as conditioning substances and nutrients are used according to a ratio so that the compound foliar fertilizer is formed. The compound foliar fertilizer contains complete nutrients and has multiple functions and strong specificity. Through use of a certain amount of a chelating agent, a surfactant or carrier, after spraying, the foliar fertilizer can be well adhered to and spread on the leaf surface so that leaf absorption and use are promoted. The rare earth elements lanthanum and cerium can effectively promote seed germination, promote crop stem growth, increase dry weight of seedlings, promote root growth, increase root activity and chlorophyll content, promote enzyme synthesis and pollen tube elongation, improve a crop seed setting rate, improve crop photosynthesis efficiency and a crop yield and improve the quality of crops. Through synergism of trace element selenium and gibberellin, a fungus inhibition capacity and crop resistance are improved, synthesis of chlorophyll is adjusted and crop quality is improved.

The invention relates to a method for taking paper pulp as raw materials and as a carbon source which is needed for synthesizing cellulose enzyme by trichoderma reesei after the paper pulp is processed with dilute sulfuric acid. Compared with a method for taking pure cellulose as the carbon source, the filter paper enzyme activity can be increased by 40% through using the method for preparing the cellulose enzyme, and the problems that somatic cell is lack of the ability to utilize cellulose and the activity of the cellulose enzyme is not high when the paper pulp and dextrose are taken as the carbon source to prepare culture medium are solved.

The fast detection process of lipase synthesis activity in nonaqueous phase belongs to the field of nonaqueous enzyme catalysis technology. Lipase is used in catalyzing the esterification between p-nitro phenol ester and alcohol in nonaqueous phase, and after reaction for certain time, reacted liquid in certain amount is extracted with 0.1M concentration NaOH solution and the absorbance at 410 nm is measured in a colorimetric process so as to determine the amount of produced p-nitro phenol. The lipase activity unit is defined as the amount of lipase required for producing 1 micromol of p-nitro phenol. The present invention is used for the direct screening of lipase producing bacteria with high ester synthesizing activity and has relatively low cost.

The invention discloses a method for recovering D-p-hydroxyphenylglycine (D-HPG) from a cefprozil production waste liquid in an enzyme synthesis process, which comprises the following two steps: 1. separating D-HPG from the crystallization waste liquid through ion exchange by using an ion exchange resin; and 2. purifying and eluting the D-HPG by using hydrochloric acid solutions with different concentrations under the actions of ion exchange and static electricity, and drying to obtain the finished product. The method is characterized in that the process of separating and purifying the D-HPG from the cefprozil production waste liquid in the enzyme synthesis process is completed by using the ion exchange resin, and meanwhile, the subsequent waste liquid treatment technique of the enzyme-process cefprozil synthesis is completed.

Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.