76 results about "Cefalexin" patented technology

The invention discloses a piece of fluorescence immunoassay chromatography test paper which is rapid, sensitive, simple and convenient to operate to test the residual quantity of cefalexin, and preparation of the test paper. The test paper comprises a sample pad, a binding pad, a nitrocellulose membrane and a water absorbing pad, which are adhered to a substrate in a mutual lap joint manner in sequence, wherein the nitrocellulose membrane is coated with a detection line and a quality control line; the binding pad is coated with a cefalexin monoclonal antibody with a fluorescent mark. The preparation method of the fluorescence immunoassay chromatography test paper for cefalexin residue comprises the following steps: synthesizing cefalexin-ovalbumin coating antigen, preparing a goat anti-mouse immune globulin antibody, coating the cefalexin-ovalbumin coating antigen and the goat anti-mouse immune globulin antibody onthe nitrocellulose membrane to serve as the detection line and the quality control line, preparing a fluorescence nano-particle mark cefalexin monoclonal antibody, then coating glass fiber to serve as the binding pad, sequentially adhering the sample pad, the binding pad, the nitrocellulose membrane and the water absorbing pad to a back plate in the lap joint manner in sequence, cutting the obtained test paper into the width of 4mm, and preserving at normal temperature.

The invention discloses a method for preparing cefalexin. The method comprises the steps of charging a D-2-phenylglycine ester derivative and 7-ADCA (amino desacetoxy cephalosporanic acid) in a molar ratio of (1.15-1.6):1, and adding a catalyst penicillin acylase with amount of 1-2 times of amount of the 7-ADCA for acylation reaction, wherein the coded gene sequence of the penicillin acylase is shown as SEQIDNO:3. The method effectively overcomes reverse reaction during enzyme condensation reaction, so as to greatly reduce the using amount of side chains, avoid the phenomenon that more impurities are generated when high side chains are consumed, and solve the problem that the objective product is difficult to purify; and besides, the 7-ADCA conversion rate can be greatly improved to be up to more than 98%, the product quality is further improved and the production cost is reduced.

The present invention relates to a preparation method for a magnetic carbon microsphere surface cephalexin molecular imprinted adsorbent material. The method comprises: taking a walnut shell as a carbon source to prepare a magnetic carbon microsphere; carrying out ethylene functionalized modification on the surface of the magnetic carbon microsphere by use of 3-(methacryloxy)propyl trimethoxysilane; taking cephalexin (CFX) as a template molecule, methacrylic acid (MAA) as a functional monomer, ethylene alcohol dimethacrylate (EGDMA) and N, N- methylene bisacrylamide (BIS) as a crosslinking agent, and cetane as a porogenic diluent and an organic solvent to prepare a magnetic carbon microsphere surface imprinted adsorbent. The magnetic carbon microsphere surface imprinted adsorbent material prepared by the method disclosed by the invention is low in cost and simple in preparation, and has high recognition, high selectivity, and high separation enrichment capacity on target molecules, and a high absorption speed.

The invention discloses a cefalexin liposome and a medicinal composition thereof. The cefalexin liposome is mainly prepared from cefalexin, phospholipid, cholesterol and poloxamer 188 in certain proportion.

A slowly-released medicine of cefa-iskia in the form of dispersing solid is prepared from cefa-iskia and slow-releasing skeleton.

The invention discloses a cefalexin tablet and a preparation method thereof. A dry granulating process is adopted, and not only is the degradation of beta-lactam rings achieved, but also the problem of low dissolution of products produced through conventional wet granulation is solved. The product is high in stability, a dissolution curve is similar to that of a reference product, and a production process is simple.

The invention discloses a method for preparing compound cefalexin injection. The composition comprises the following components in parts by weight: 2-5 parts of cefalexin, 1-2 parts of protostemonine, 1-5 parts of PVC (Polyvinyl Chloride), 1-2 parts of ethyecellulose, 1-3 parts of lecithin, 0.5-1 part of sorbitan fatty acid, 0.5-1 part of polysorbate and 1-3 parts of PEG. The composition disclosed by the invention is high in drug stability, high in solubility and simple in preparation process.

The invention discloses an antibody chip kit for detecting residual cephalosporin antibiotics in food, and belongs to the technical field of the drug residue detection. The kit comprises a chip, an antibody, a second antibody marked by Cy3, and an extraction reagent, the antibody is composed of a cefalexin monoclonal antibody and a ceftiofur monoclonal antibody, and the cefalexin monoclonal antibody is secreted by hybridoma 3A6 with a preservation number of CCTCC NO: C201340; the ceftiofur monoclonal antibody is secreted by hybridoma 4D5 with a preservation number of CCTCC NO: C201341, and the chip fixes cefalexin coating antigen and cefalexin coating antigen. The invention further discloses an antibody chip method for detecting residual cephalosporin antibiotics in food, the method can be used for simultaneously detecting 8 kinds of cephalosporins, and has the advantages of high accuracy, high precision, high efficiency, etc.

The invention provides an effective method for accurately and objectively measuring resistant starch content in rice by adding different types of antibiotics into starch hydrolyzate to discuss the effect of the antibiotics on starch hydrolysis and influence on a measuring result of the resistant starch content. By comparing the influences of the antibiotics on the starch hydrolysis effect and the resistant starch content of a detected sample, a result shows that the fermentation effect of miscellaneous bacteria in the starch hydrolysis process can be effectively suppressed by the antibiotics without affecting the activity of amylase; the most effective antibiotic is mixed antibiotic (four antibiotics, namely 0.40-0.52 mg/mL of penicillin, 15,000-17,000 UI/mL of gentamicin sulphate, 0.8-1.2 mg/mL of cefalexin and 0.85-1.30 mg/mL of erythromycin lactobionate, are mixed according to a proportion of 1.2:1:1.2:1 ); and the best dose is 35 microlitre.

The invention relates to a method for concentrating cefalexin, in particular to the method for concentrating cefalexin through a membrane method and an enzyme concentration method. The method mainly solves the problem that the environment is polluted and energy dissipation is high due to the fact that the existing method for concentrating the cefalexin needs to use the extracting solvent. The method comprises the steps that 1, cefalexin mother solution prepared by the enzyme concentration method is stored into a mother solution storing tank; 2, the cefalexin mother solution passes through a refined filtration unit; 3, the cefalexin mother solution passes through an ultrafiltration membrane unit; 4, the cefalexin mother solution passes through a nanofiltration membrane unit unit; 5, the cefalexin mother solution is conveyed to a reverse osmosis membrane unit, and then the concentrated cefalexin is obtained. By means of the method, the original cefalexin solution can be concentrated more than 10 times, energy dissipation is low, alternative use of the extracting solvent is greatly reduced, harm to human bodies is reduced, and pollution to the environment is avoided. The method is used for concentrating the cefalexin.

The invention discloses cefalexin capsules and a preparation method. The capsules comprise the following components in percentage: 90.6% of cefalexin, 6.4-8.3% of microcrystalline cellulose, 0.6-1.6%of carboxymethyl cellulose sodium and 0.5-1.4% of magnesium stearate, wherein the sum of weight percentages of the components is 100%. The preparation process of the capsules comprises the following steps: performing pre-mixing for 16 minutes at a frequency of 10Hz, performing pelletizing, performing batch mixing for 10 minutes at a frequency of 10Hz, and performing capsule filling, wherein pelletizing conditions are that the stirring speed is 25rpm, the spiral rotation speed is 55-85rpm, the rotation speed of rollers is 16rpm, the gap of the rollers is 0.9-1.3mm, the rotation speed of granuleshaping is 80rpm, the pressure of the rollers is 100-130bar, the aperture of a granule shaping mesh size is 1.0mm, and the vacuum degree is 0.1bar. The flowability and the dissolution stability of the capsules disclosed by the invention can be improved, and the method for preparing the capsules is low in energy consumption and green and environment-friendly.

The invention discloses a specific monoclonal antibody capable of distinguishing cefalexin, cefadroxil and cefradine, and an enzyme-linked immunosorbent assay method and kit for detecting the cefalexin, the cefadroxil and the cefradine. According to the invention, the monoclonal antibody is secreted by a hybridoma cell strain 3A6 of which the preservation number is CCTCC No.C201340. Compared with the prior art, the monoclonal antibody, prepared by the invention, can be used for distinguishing the cefalexin, the cefadroxil and the cefradine at the same time. The enzyme-linked immunosorbent assay method and kit disclosed by the invention have the advantages of high detection efficiency, high sensitivity, high precision, high accuracy and the like.

The invention discloses a COF-TpPa-SO3H membrane with efficient nanofiltration separation effect on the antibiotic cefalexin, belonging to the field of novel membrane materials. A preparation method for the membrane comprises the following steps: firstly, acquiring a great number of COF nanosheets with a high area-thickness ratio by using the hydrophilicity of sulfonate radicals in a molecular structure through a simple interfacial polymerization method, and then carrying out vacuum filtration in a layer-by-layer stacking manner to obtain the COF-TpPa-SO3H membrane with high mechanical strength and high chemical stability. The removal rate of the pure COF-TpPa-SO3H membrane prepared by vacuum filtration at normal temperature on the antibiotic cefalexin is as high as 95%, with an error range being +/- 0.6%; and a water flux is up to 800 L.m<-2>.H<-1>.MPa<-1>, and the highest water flux of the obtained pure COF membrane is 2500 L.m<-2>.H<-1>.MPa<-1> by changing the deposition mass of COF. The water flux of the COF membrane prepared by the method is higher than the water flux of a currently researched GO nanofiltration membrane and other ultrathin two-dimensional material membranes, and the COF membrane has good application potential in antibiotic removal.

The invention relates to a compound cefalexin capsule and a preparation method thereof. The compound cefalexin capsule is prepared from the following raw materials in parts by weight: 220-270 parts of cefalexin, 2-20 parts of ambroxol, 25-125 parts of polacrilin potassium, 50-100 parts of chito-oligosaccharide, 5-50 parts of talc and 10-75 parts of starch. The compound cefalexin capsule is reasonable in formula, high in drug dissolution, simple in preparation method without pollution and remarkable in efficacy without adverse reaction.

The invention belongs to the technical field of treatment of antibiotics and particularly discloses an application of a V2O5/CeO2 composite nano-material in degradation of cefalexin-containing wastewater. The application comprises the steps of adjusting the initial concentration of cefalexin in the wastewater to 20mg/L to 120mg/L, adjusting the pH value of the wastewater to 2 to 7, then, adding 5mg to 30mg of the V2O5/CeO2 composite nano-material into per 20mL of wastewater, and finally, carrying out isothermal oscillation for 1 to 6 hours at the temperature of 25 DEG C to 70 DEG C. The V2O5/CeO2 composite nano-material has an obvious oxidative catalysis action on cefalexin antibiotic, the degradation effect is optimal under the conditions that the concentration of the wastewater is 40mg/L, the pH of the wastewater is 3, the dosage is 20mg, the temperature is 50 DEG C, and the oscillation time is 2 hours, and the removal efficiency can reach 64.54% to 69.13%. Under the optimal degradation conditions of the V2O5/CeO2 composite nano-material, the V2O5/CeO2 composite nano-material has better effect and higher efficiency to the degradation of the cefalexin antibiotic compared with pure nano-CeO2.

The invention relates to a process for the preparation of cephalexin with the aid of a penicillin amidase immobilized on a crosslinked hydrophilic copolymer which has binding activity for ligands having nucleophilic groups and is in bead form.

The present invention discloses salvianolic acid A and a novel use of pharmaceutical composition comprising salvianolic acid A. The invention is characterized by the application of salvianolic acid A in preparing the medicament for treating skin injury, and the application of pharmaceutical composition composed of salvianolic acid A, snow grass, pinellia tuber, phellodendron amurense, cefuroxime, cefalexin, ceftriaxone sodium, etc. in preparing the medicament for treating skin injury. The pharmaceutical and clinical researches show that the salvianolic acid A and pharmaceutical composition comprising salvianolic acid A have excellent treating function for skin injury.