Fluorescence immunoassay chromatography test paper for cefalexin residue and preparation of test paper
A technology of fluorescence immunochromatography and cephalexin, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of rapid detection of unsuitable samples, high cost, cumbersome measurement operation, etc., and achieve simple measurement method, simple processing process and high sensitivity high effect
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[0034] Embodiment 1: Preparation of cephalexin residual fluorescent immunochromatographic detection test strip
[0035] 1. Synthesis of Protein-Coupled Antigens
[0036](1) Synthesis of cephalexin immune antigen
[0037] The immune antigen was obtained by coupling cephalexin and bovine serum albumin (BSA) by carbodiimide method.
[0038] The specific operation is as follows: Dissolve 15 mg of cephalexin in 5 mL of PBS (0.01 mol / L, pH 7.4), add 25 mg of carbodiimide (EDC) and 10 mg of N-hydroxysuccinimide (NHS) and stir for 30 minutes, and take 10 mg of BSA Dissolve in activated cephalexin solution, react with stirring at 25°C for 2 hours, then react overnight at 4°C. Dialyzed with PBS for 3 days at 4°C to obtain the cephalexin immune antigen, which was subpackaged and frozen.
[0039] (2) Synthesis of cephalexin-coated antigen
[0040] Coupling of cephalexin and ovalbumin (OVA) was carried out by glutaraldehyde method to obtain the coated antigen.
[0041] Concrete operat...
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