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Preparation method of ratiometric fluorescent probe for detecting cefalexin residues and fluorescent probe prepared by preparation method and application thereof

A ratio fluorescent probe, cephalexin technology, applied in fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc., can solve the problems of large interference, complicated detection operation of cephalexin, expensive instruments and detection environment, etc., to achieve control The effect of food safety, protection of human health, and fast detection speed

Active Publication Date: 2020-09-11
NANJING NORMAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Purpose of the invention: Aiming at the problems existing in the prior art, the present invention provides a method for preparing a ratiometric fluorescent probe for detecting cephalexin residues. The probe prepared by the present invention can effectively solve the problem of complex detection operations or expensive instruments for existing cephalexin detection. Or detect defects that are more affected by environmental interference

Method used

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  • Preparation method of ratiometric fluorescent probe for detecting cefalexin residues and fluorescent probe prepared by preparation method and application thereof
  • Preparation method of ratiometric fluorescent probe for detecting cefalexin residues and fluorescent probe prepared by preparation method and application thereof
  • Preparation method of ratiometric fluorescent probe for detecting cefalexin residues and fluorescent probe prepared by preparation method and application thereof

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Embodiment 1

[0036] Detect the preparation of cephalexin fluorescent probe, comprising the following steps:

[0037] (1) Preparation of carbon dots: 2.0 g Tris and 0.125 g lactose were dissolved in 40 mL of purified water. Under magnetic stirring, the mixed solution was heated to reflux at 100° C. for 24 hours, and the color changed from colorless to yellow or even brown. After cooling to room temperature, neutralize the pH value to 7 with hydrochloric acid, and store at 4°C until use to obtain a blue fluorescent carbon quantum dot solution.

[0038] (2) Preparation of cadmium telluride quantum dots: Add 0.25mmol cadmium chloride, 0.6mmol mercaptopropionic acid, 180mL pure water into a 250mL three-neck round bottom flask, and then adjust the pH to 10 with 1mol / L NaOH. Add 0.0636g Te powder, 0.363g sodium borohydride NaBH to a 10mL round bottom flask 4, and 8mL of pure water, passed through argon and magnetically stirred at room temperature for about 20-30min. When the solution changed fr...

Embodiment 2

[0058] The preparation method of embodiment 2 is identical with embodiment 1, difference is: the mass ratio of Tris described in step (1) and lactose is 15:1; The mol ratio of tellurium powder described in step (2), sodium borohydride is 1:15, reflux reaction at 100°C for 24 hours; the volume ratio of TEOS to APTES in step (3) is 2:1, the volume ratio of carbon quantum dot solution to TEOS is 70:1, and the volume ratio of TEOS to ammonia water is 1:1.5 ; The silicon-coated carbon quantum dot solution and the cadmium telluride quantum dot solution mass ratio of step (4) are 1:3, the cephalexin antibody solution and solution A, the second addition and 1-(3-dimethylamino The volume ratio of the mixed solution of propyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide is 3:30:5, and the concentration of the cephalexin antibody solution is 10 ug / mL.

Embodiment 3

[0060] The preparation method of embodiment 3 is identical with embodiment 1, and difference is: the mol ratio of tellurium powder described in step (2), sodium borohydride is 2:3; The carbon quantum dot solution described in step (3) reacts with TEOS The volume ratio is 60:1, and the volume ratio of TEOS to ammonia water is 1:1.8.

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Abstract

The invention discloses a preparation method of a ratiometric fluorescent probe for detecting cefalexin residues, the fluorescent probe prepared by the preparation method and application. The preparation method comprises the following steps of: preparing carbon quantum dots, preparing cadmium telluride quantum dots, preparing silicon-coated carbon dots and synthesizing the fluorescent probe. The preparation process is simple, raw materials are easily available, the cost is low, and large-scale production is easy. The ratiometric fluorescent probe for detecting the residual cefalexin is high insensitivity, has high selectivity and specificity, only has fluorescence response to the cefalexin, and has no response to other antibiotics. The ratiometric fluorescent probe for detecting the cefalexin residues, prepared by the method, can be used for effectively and quantitatively detecting the cefalexin residues, is more efficient, cost-saving and more accurate, and has important significancein controlling food safety and protecting human health.

Description

technical field [0001] The invention belongs to the fields of drug analysis and food safety, in particular to a preparation method of a ratio fluorescent probe for detecting cephalexin residues, the prepared fluorescent probe and its application. Background technique [0002] Cephalexin is the first-generation cephalosporin with a broad antibacterial spectrum and is widely used to treat diseases such as urinary tract infection, skin infection, respiratory tract infection and mastitis. With the widespread application of cephalexin in animal husbandry, it is inevitable that there will be antibiotic residues in animal foods, and the existence of these residues should attract people's attention, because of its potential carcinogenic properties, which may lead to antibiotic resistance and people's allergic reactions. In recent years, many detection methods have developed rapidly, such as high-performance liquid chromatography, liquid chromatography-mass chromatography, and enzym...

Claims

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Application Information

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IPC IPC(8): C09K11/88C09K11/65C09K11/02G01N21/64
CPCC09K11/883C09K11/65C09K11/025G01N21/6428G01N2021/6432
Inventor 杨雅琼郝爱月梅艳珍戴传超
Owner NANJING NORMAL UNIVERSITY
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