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Preparation method of immune chromatography test paper for detecting cephalosporin antibiotic

A technology of immunochromatography test paper and antibiotics, which is applied in the field of immunological detection, can solve the problems of not being able to adapt to high-throughput rapid detection, and achieve the effect of easy operation

Inactive Publication Date: 2008-12-03
JIANGNAN UNIV
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Problems solved by technology

However, these detection methods require expensive instruments and equipment, professional and technical personnel are required for operation, and cannot be adapted to high-throughput, on-site rapid detection

Method used

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  • Preparation method of immune chromatography test paper for detecting cephalosporin antibiotic

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Embodiment 1

[0015] One end of the PVC backing is pasted with a sample pad and a conjugation pad in turn, and a nitrocellulose membrane is adhered in the middle, and an absorbent pad is adhered to the other end. The membrane was coated with cephalexin-OVA, and goat anti-rabbit IgG.

[0016] Prepare according to the following steps: (1) Prepare cephalexin-OVA conjugate: dissolve cephalexin and OVA in PBS (0.01mol / L, pH7.4) with a molar ratio of 100:1, add glutaraldehyde under stirring reagent, reacted for 3 hours, and dialyzed at 4°C for 3 days. Formation of cephalexin-OVA conjugates;

[0017] (2) Preparation of colloidal gold: reducing chloroauric acid with trisodium citrate reducing agent to make 20nm-40nm colloidal gold particles;

[0018] (3) Preparation of polyclonal antibody-colloidal gold marker: adjust the colloidal gold to about pH 9, add the optimal amount of polyclonal antibody drop by drop under stirring, add an appropriate amount of BSA after standing for 30 minutes, leave fo...

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Abstract

The invention provides a method for preparing an immunity chromatographic test strip for testing cephalosporins antibiotics, and relates to the immunity testing field. The test strip is composed of a sample pad, a combined pad, a cellulose nitrate membrane, a water absorbing pad and a PVC backing; the sample pad, the combined pad, the cellulose nitrate membrane and the water absorbing pad are sequentially stuck to the PVC backing; the combined pad is coated with a group-specific anti-cephalosporins antibiotic antibody colloidal gold label of anti-cephalosporins antibiotics; and the cellulose nitrate membrane is sequentially coated with a cefalexin-OVA test line and a goat anti-rabbit IgG quality control line. The method prepares the test trip by assembling the test preparation of the combination of the group-specific antibody of the anti-cephalosporins antibiotics and the chromogenic colloidal gold, and carries out a multi-residue screening test of the cephalosporins antibiotics with low cost and rapidness; and the portable test strip which can be used for the cephalosporins antibiotic residue tests of different samples is the application of the immunity chromatographic analysis method.

Description

technical field [0001] The invention relates to a preparation method of an immunochromatographic test strip for detecting cephalosporin antibiotics, and the invention relates to the field of immunological detection, in particular to the preparation of an immune colloidal gold reagent and a test strip. Background technique [0002] Because there are many kinds of veterinary drugs, there are also many kinds of cephalosporin antibiotics, and the single residue detection is far from being able to meet the good detection of drug residues caused by the abuse of veterinary drugs, and for the production of fermented milk, at least one class of antibiotics can be detected. is very useful. Existing methods that can realize multi-residue detection are mainly instrumental analysis, including high performance liquid chromatography, liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), and the like. However, these detection methods require expen...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N33/558
Inventor 胥传来谢会玲刘丽强晁慧楠
Owner JIANGNAN UNIV
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