The invention discloses a method for extracting and purifying a lignin peroxidase by using reverse micelles. The method comprises the following steps of: preparing a reverse micelle solution containing a biosurfactant, isooctane and n-hexyl alcohol, wherein the biosurfactant is rhamnolipid; mixing a prepared crude solution of the lignin peroxidase with the reverse micelle solution, stirring, centrifuging, then, standing to carry out phase splitting, and distilling to obtain an upper organic phase; and mixing the upper organic phase with a buffer solution, stirring, centrifuging, then, standing to carry out phase splitting, distilling to obtain a lower water phase, i.e. a purified lignin peroxidase solution. The method disclosed by the invention has the advantages of environment-friendliness and safety, simplicity and convenience in operation, and low cost.