180 results about "Xylanase Y" patented technology

The present invention discloses methods of bleaching chemical pulp that combine xylanase enzymes with hydrogen peroxide, peracids, or a mixture. The method comprises the steps of carrying out a chemical pulping operation, optionally followed by delignifying the pulp with oxygen, then combining xylanase enzymes with hydrogen peroxide, peracids, or a mixture to bleach the pulp. The method allows the mill to use both xylanase and peracids in a single bleaching tower to decrease the usage of chlorine dioxide and other bleaching chemicals. The pulp bleaching method of the present invention may be performed in a pulp mill as part of a complex pulp bleaching process.

The invention discloses a composite plant enzyme containing probiotics and an application of the composite plant enzyme. The composite plant enzyme comprises amylase, lipase, protease, cellulase, beta-dextranase, xylanase and lactase. The composite plant enzyme is obtained by discontinuously feeding materials and fermenting for a plurality of periods, selecting a plurality of types of fruits and vegetables, and edible and medical plants as fermentation substrates and taking a plurality of types of probiotics/yeasts as fermentation inoculation substances through a plurality of grades of deep fermentation. The composite plant enzyme contains seven enzymes needed by a human body at the same time so that the plant enzyme has the synergistic effects of promoting in-vivo metabolism and decomposing toxic substances; the plant enzyme contains a high-activity compound enzyme and has a wide acting condition, good adaptability, and good stability, acid-base resistance and high-temperature resistance; the plant enzyme can effectively and rapidly improve the biochemical reaction speed and can be used as a raw material to be applied to foods and chemicals for daily use.

The invention provides a method for producing ethanol by synchronizing saccharification, isomerization and fermentation of lignocellulose. In the method, cellulase, xylanase and xylose isomerase are used to enzymolyze the lignocellulose, and enzymolysis products are fermented to obtain the ethanol. The method adopts the synchronous saccharification, isomerization and fermentation of the lignocelluloses to produce the ethanol, and has the advantages of reducing the negative feedback inhibition of the enzymolysis products on the cellulase and the xylanase, increasing enzymolysis rate, shortening a fermentation period, reducing production cost and the like.

The present invention relates to re-compounded enzyme preparation and its application in cotton fabric treatment, and is especially one bath and two step enzyme process for pre-treating cotton fabric. Alkaline pectase, cellulase, alpha-amylase, PVA degrading enzyme, proteinase, lipase, xylanase and sugar oxidase are first re-compounded, and the re-compounded enzyme preparation is then used in the one bath and two step enzyme process for pre-treating cotton fabric. The process includes the first desizing and boiling off step in the treating bath at 55 deg.c and pH 8.0 for 60-90 min, with the sugar oxidase reacting with the desizing product to produce hydrogen peroxide; and the second fabric bleaching step with the enzyme process generated hydrogen peroxide at 95 deg.c and pH 11.0 for 60-120min. The present invention has low power consumption, short technological process and environment friendship, and may be used to replace chemical process.

The invention provides a modified coding gene of xylanase that has high activity in the condition of high temperature and strong alkali, a recombinant plasmid thereof, a yeast recombination genetic engineering strain containing the gene and a preparation method of the gene. Compared with the original xylanase, the modified xylanase is mutated into cysteine from glycin of the 201 site of amino acid sequence of the original xylanase; and the modified xylanase has higher heat stability, after being processed for 10 minutes at the temperature of 75 DEG C, the residual activity of the modified xylanase reaches 80 percent, while that of the original xylanase is only 15 percent. The activity of the modified xylanase is 1.5 to 2 times of that of the original xylanase at the environment with high temperature and strong alkali and can decompose xylan for a long time at high temperature. The invention also provides the Pichia pastoris yeast recombination genetic engineering strain containing the modified gene. The modified xylanase can be widely applied to industries of paper making, feedstuff and food.

A method for preparing a bamboo fiber by a compound bio-enzyme belongs to the technical field of manufacturing the natural cellulose fiber used for weaving. Bamboo linen is used as raw material; the bamboo linen normally processed is treated in the water liquid of the compound bio-enzyme for 1 to 5 hours, then is treated in a pectase liquid for 1 to 5 hours, then the bamboo fiber is obtained after normal processing; the water liquid of the compound bio-enzyme is any single or mixture of laccase, xylanase, the mixture of the laccase and the xylanase, the mixture of the laccase and cellulose, the mixture of the xylanase and the cellulose as well as the mixture of the laccase, the xylanase and the cellulose; the concentration of the laccase and the xylanase are respectively 20 to 50g/L; the concentration of the cellulose is 2 to 6g/L; the concentration of the pectase in the pectase liquid is 10 to 25g/L. The technique of the method is reasonably designed; the fineness, the intensity and the softness of the prepared bamboo fiber can all meet the weaving requirements.

The invention relates to a process for preparation of fermentation and induction of ramie degumming compound enzyme, which includes the steps of (1) induction preparation of ramie degumming compound enzyme system of trichoderma reesei, (2) determination of the activity of cellulose, xylanase and pectinase and (3) the processing of ramie by crude enzyme liquid which is obtained by fermentation. The invention can obtain the higher enzyme activity, is better qualified to the compound enzyme system of the ramie degumming, and is low in costs, simple and convenient in operation and easy in industrial production.

The invention discloses a preparation method of superfine bran powder. The method comprises the following steps: (1) steam explosion, namely, feeding bran into a steam explosion tank, maintaining for 10 to 12 minutes at the temperature of 160 to 180 DEG C and under the pressure of 0.6 to 1.0MPa, and then performing instantaneous explosion; (2) enzymolysis, namely, collecting bran subjected to steam explosion in step (1), adding water, and then adding a mixture of cellulose, hemicellulase, xylanase and pectinase, wherein 0.5 to 1U/g of each of cellulose, hemicellulase, xylanase and pectinase is added to the mixture of bran and water; (3) crushing, namely, performing superfine crushing for the mixture obtained in step (2); and (4) drying, namely, drying the mixture obtained in step (3) to obtain the superfine bran powder. With the adoption of the method, cellulose, hemicelluloses, xylan, pectin and other high molecular substances with high content in the bran can be fully degraded, so that the problems of poor taste and difficult digesting of the superfine bran can be solved, and moreover, the energy consumption in the superfine bran crushing process can be effectively reduced.

The invention discloses an enzyme scouring agent for linen fibers. The enzyme scouring agent contains 2%-3% by volume of pectinase, 2%-4% by volume of glucanase, 2%-3% by volume of cellulose, as well as a 4-5g/L surfactant scouring agent and a 2-3g/L chelating agent, wherein a bath ratio is 1:30. The linen fibers are scoured by compound enzyme, so that the effect of removing impurities of pectin and lignin is very good; besides, the surfactant scouring agent and the chelating agent are added, thereby further reinforcing the impurity removing effect of the compound enzyme and effectively reducing the use amount of the compound enzyme; in addition, the scoured linen fibers achieve a degumming degree of 20%-23% and have a good handfeel, excellent whiteness and a fine capillary effect.

The invention provides a special complex enzyme preparation for wheat for livestock and poultry and application thereof. The special complex enzyme preparation comprises xylanase, beta-glucanase, beta-mannase, cellulase, acid protease, neutral protease, pectinase, alpha-galactosidase, glucoamylase and aspergillus niger fermentation complex enzyme; and an antioxidant, a mildew-proof agent, an insect repellant and a carrier are added. According to the invention, the special complex enzyme for wheat is prepared from xylanase for effectively reducing viscosity and mono-enzymes such as beta-glucanase, beta-mannase, cellulase, acid protease and the like; and the preparation is simpler than the operation of preparing a complex enzyme by fermenting aspergillus niger only, has low labor capacity and cost, and realizes a good enzymolysis and viscosity reduction effect. Through the invention, the anti-nutrition effect caused by adding high-proportion wheat in daily ration can be effectively overcome, the chyme viscosity is reduced, the production performance of animals is enhanced, the utilization of wheat and wheat enzyme is optimized for a feed enterprise, and the feed cost is effectively lowered.

The invention relates to a steamed bun modifier, which comprises following raw materials by mass percent: 90-96% of corn starch, 1-5% of monocalcium phosphate, 1-5% of calcium sulfate, 1-5% of vitamin C, 0.1-0.5% of fungus amylase, 0.1-0.5% of lipase, 0.1-0.5% of xylanase, and 0.1-0.5% of notatin. The steamed bun modifier has the advantages of containing no inhibited calcium peroxide, thoroughly solving the problem in production that the steamed bun made by the modifier without calcium peroxide has no uniform tissue, is not compact, is not soft, has poor elasticity, is not tough and soft in taste, and has short service life, improving the product quality, and also improving the production efficiency and quality of the product.

A method for preparing oligoxylose and alpha-glucose aldehydic acid enzyme is disclosed. It includes: saccharogenic decomposing xylan by combination of xylanase and alpha-glucose aldehydic acid enzyme, expanding and obtainingalpha-glucose aldehydic acid enzyme gene from donor bacteria by PCR, inserting carrier, constructed recombinant plasmid converting into host cell and expressing it, centrifugal obtaining supernatant fluid(enzyme liquid) by centrifugal collecting cell, breaking cell and heat treating. It achieves high zymoprotein output, activity and purification.

The invention discloses a high plant cell wall degradation activity rumen fungi, and applications thereof in feed ensiling, and belongs to the technical field of microorganism and feed processing. According to a preparation method, Hungate anaerobic roll-tube technique is adopted to obtain an anaerobic fungi with cell wall degradation enzyme activity via separating and selecting from the rumens of Xinong Saanen dairy goats, finally obtained Piromyces sp.CN6 is capable of producing xylanase and acetylesterase with relatively high activity in a wide temperature and pH range, and excellent thermal stability and acidic and alkaline stability, the preservation number is CGMCC No.14449. The bacteria strain is capable of improving fermentation quality of silage, increasing crude fiber degradation rate, and can be applied in practical production as a silage additive.

The invention discloses a liquid complex enzyme for peeling membranes of orange segments. The liquid complex enzyme is characterized by being formed by compounding a component A, xylanase and beta-glucanase, wherein the volume of the xylanase is equal to that of the beta-glucanase; the volume ratio of the xylanase to the component A and the volume ratio of the beta-glucanase to the component A are 1:9 to 1:4; the component A is formed by mixing acidic pectinase and acidic cellulase in the volume ratio of (4-1):(1-4); and the xylanase, the beta-glucanase, the acidic pectinase and the acidic cellulose are qualified food grade liquid commodity enzymes. The invention also discloses the compounding process of the liquid complex enzyme and the application process of the liquid complex enzyme toorange can processing and production. The liquid complex enzyme for peeling the membranes of the orange segments can greatly improve production efficiency, reduce the breaking rate of the orange segments, stabilize production quality and lower production cost, has the advantages of no residue, environmental friendliness, high safety and no environmental pollution, is easy and convenient to use, efficiently and completely works on the membranes of the orange segments, decomposes thoroughly and does not damage internal pulp vesicles.

The invention provides a preparation method of biological feed. The method comprises the following steps of crushing stalks; mixing crushed stalks with wheat bran; adding xylanase and cellulase; then, supplanting and adding water accounting for 75 to 85 percent of the weight of the stalks; then, inoculating lactobacillus liquid and yeast liquid; obtaining the biological feed after the enzymolysis and fermentation. Through the optimized enzymolysis combination, the microbial combination anaerobic fermentation is combined; the water content is reduced to a proper degree, so that the treatment cost of per ton of stalks is reduced by about 300 Yuan; the practical application values are basically realized. The prepared biological feed uses the weight ratio of 20 percent for replacing the complete formula feed; the growth speed, differences of the feed conversion ratio and the like of live pigs are not obvious, but the grain consumption and the live pig weight increase cost are obviously reduced; the contents of pork protein, amino acid and calcium can be increased; the contents of fat and cholesterol are reduced.

The invention relates to a method for brewing wheat beer by using ungerminated wheat for matching with wheat malt, which comprises the steps of pasting, mashing, filtration of wheat juice, boiling of the wheat juice, precipitation of the wheat juice, fermentation and filtration of the beer. The pasting process adopts rice which accounts for 30-40% of total weight of dry materials and wheat which accounts for 25-35% as raw materials, and 180-200ppm of high-temperature-resistant amylase, protease pentopan, xylanase and gypsum during the pasting process. The method takes the ungerminated wheat as an auxiliary material for brewing the beer, thereby reducing the production cost. The pentopan and the protease are added during the pasting process, the temperature is rapidly increased to the temperature of inactivating oxidase for carrying out pasting and liquefaction after the transient pentosan and protein stop, thereby overcoming the phenomenon that the wheat is added in a mashing kettle, the oxidase promotes the oxidation of pigment substances because that the process is not processed at high temperature, thereby blackening the wheat juice.