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Fermentation inducement preparation method for ramee degumming composite enzyme

A technology of hemp degumming enzyme and compound enzyme, which is applied in the field of fermentation induction and preparation of ramie degumming compound enzyme, can solve the problems of not fully considering the effect of cellulose, and achieve convenient and easy price, strong controllability, and mild reaction conditions Effect

Inactive Publication Date: 2008-05-28
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research only focuses on pectin and hemicellulose (xylan), without fully considering the role of cellulose in pectin.

Method used

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  • Fermentation inducement preparation method for ramee degumming composite enzyme
  • Fermentation inducement preparation method for ramee degumming composite enzyme
  • Fermentation inducement preparation method for ramee degumming composite enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Determination of chemical components of ramie

[0040] The test adopts the quantitative analysis method of ramie chemical composition in the national standard GB5889-86 of the People's Republic of China to analyze the raw ramie used in this test. The results obtained are shown in Table 1, which are quite consistent with the results reported in the literature.

[0041] Table 1 Quantitative analysis of chemical components of ramie

[0042] Chemical composition of ramie

The measurement results(%)

Literature (%)

moisture

Cellulose

Fat waxy

lignin

pectin

Hemicellulose

total gum

7.02

71.47

0.88

2.17

3.89

13.93

25.74

-

70-75

0.538

1.19

4.043

13.287

25-30

[0043] 2. Seed Culture of Trichoderma reesei

[0044] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015...

Embodiment 2

[0073] 1. Seed Culture of Trichoderma reesei

[0074] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N;

[0075] (2) Trichoderma reesei Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 48 hours.

[0076] 2. Fermentation of Trichoderma reesei to produce enzymes

[0077] (1) Trichoderma reesei enzyme production medium: 0.5% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N, adding 2% ramie powder, pH5.0;

[0078] (2) With 10% inoculum size, the culture without ramie powder was used as a control, and the enzyme was induced to be produced by culturing at 30° C. and 160 r / min for 7 days;

[0079] (3) The fermented broth clear liquid collected by filtration or centrifugation is the ramie degumming compound enzyme liquid.

[0080] 3. Determination of cellulase activity, see Example 1 for details.

[0081] 4. Determination of xylanase activity, ...

Embodiment 3

[0085] 1. Seed Culture of Trichoderma reesei

[0086] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N;

[0087] (2) Trichoderma reesei Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 48 hours.

[0088] 2. Fermentation of Trichoderma reesei to produce enzymes

[0089] (1) Trichoderma reesei enzyme production medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N, pH5.0;

[0090] (2) With 10% inoculum size, culture at 30°C and 130r / min for 7h to induce enzyme production;

[0091] (3) The fermented broth clear liquid collected by filtration or centrifugation is the ramie degumming compound enzyme liquid.

[0092] 3. Determination of cellulase activity, see Example 1 for details.

[0093] 4. Determination of xylanase activity, see Example 1 for details.

[0094] 5. Determination of pectinase activity, see Example 1 for details.

[...

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Abstract

The invention relates to a process for preparation of fermentation and induction of ramie degumming compound enzyme, which includes the steps of (1) induction preparation of ramie degumming compound enzyme system of trichoderma reesei, (2) determination of the activity of cellulose, xylanase and pectinase and (3) the processing of ramie by crude enzyme liquid which is obtained by fermentation. The invention can obtain the higher enzyme activity, is better qualified to the compound enzyme system of the ramie degumming, and is low in costs, simple and convenient in operation and easy in industrial production.

Description

technical field [0001] The invention belongs to the field of enzyme fermentation, in particular to a method for preparing ramie degumming compound enzyme by fermentation induction. Background technique [0002] Ramie, known as "Chinese grass" in the past, has the characteristics of fast moisture absorption and heat dissipation, ventilation and breathability, easy washing and quick drying, cool wearing, high strength, antibacterial and antiseptic properties, large expansion rate, and low electrical conductivity. Therefore, ramie textiles have always been popular. One of the precious textiles. Ramie is similar to its fellow fibers in that they are all stem fibers. But in other aspects, there are both differences and many shortcomings, which cause many difficulties in the utilization of ramie: for example, other hemp can separate the bast fiber from the central xylem as long as the stem is retting with water, but ramie cannot. The tannins of ramie stems have strong antiseptic...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12N1/14D01C1/00C12S3/06C12R1/885
Inventor 洪枫钟坤
Owner DONGHUA UNIV
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