PCT No. PCT / JP96 / 02998 Sec. 371 Date Jun. 10, 1997 Sec. 102(e) Date Jun. 10, 1997 PCT Filed Oct. 16, 1996 PCT Pub. No. WO97 / 14295 PCT Pub. Date Apr. 24, 1997The present invention provides a method for clone-multiplication of a
Paphiopedilum wherein a scape terminal
bud or an axillary
shoot, whose surface has been sterilized, or an aseptic
shoot obtained by an aseptic cultivation, of a
Paphiopedilum stock, are
cut into cross-sectional pieces which are then cultivated in a medium containing a
plant hormone. By
cutting an aseptic
shoot containing a number of axillary buds into cross-sectional pieces and cultivating, a multishoot formation is feasible. Also by employing a
liquid medium containing a
solid support such as a rockwool or a
paper filter in the culture, the
plant death due to
browning of tissues can effectively be prevented. In addition, by optimizing the method for sterilizing the buds before blooming, the medium composition and the culture condition, a large scale production of Paphiopedilums by means of a clone multiplication technology becomes feasible.