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Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme

A technology of Pallidum pallidus and straw, which is applied in the field of Pallidinus pallidus, can solve problems such as difficulties, complex chemical structures, and difficult degradation

Inactive Publication Date: 2007-03-21
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many microorganisms that can utilize cellulose and hemicellulose, and many microorganisms can also utilize lignin, they are relatively difficult
Such as the strains disclosed in the Trichoderma reesei patent and the strains reported in the literature of Trichoderma viride, a significant defect of it is that the straw is difficult to degrade under normal conditions, mainly because the cellulose, hemicellulose in the straw Combination with lignin: Lignin and hemicellulose are covalently bonded to embed cellulose molecules in the middle, making it difficult for enzymes that degrade cellulose to come into contact with cellulose molecules
The water insolubility and complex chemical structure of lignin also bring great difficulties to degradation

Method used

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  • Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme
  • Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme
  • Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Fermentation test of Pallidum pallidus:

[0065] Pick a single colony of Pallidum human from the plate and inoculate it into the seed medium of M9+ bagasse (5g / L), the pH of the seed medium is 6.7, the filling volume is 50ml / 250ml shake flask, the conditions of 30°C and 200rmp The number of cells can reach 8.0*10 in shake flasks for four days 9 about. Take 0.5ml and inoculate it into the fermentation medium of M9+bagasse (5g / L). The fermentation conditions are the same as those of seeds. After five days of cultivation, the number of cells can reach 1.4*10 10 . The total amount of starch, crude protein, soluble sugar and uronic acid contained in the bagasse we prepared is 102mg / g, and these small molecular substances that can be used for bacterial growth can only be used for bacterial growth up to 10 9 Left and right, it can be seen that the bacteria also utilize macromolecular substances such as lignocellulose. In addition, the bacteria we have tested can produce ce...

Embodiment 2～4

[0072] Comparative experiment on the growth of Pallidum hominis in "modified M9 medium" with boiled, acid-treated or alkali-treated bagasse as the sole carbon source

[0073] Bagasse 1: Bagasse—boil for 30 minutes (volume of water: bagasse=2:1) ​​and boil three times continuously—dry at 65°C—crushed—pass through a 40-mesh sieve.

[0074] Bagasse 2: Bagasse—in hydrochloric acid with a weight concentration of 20%, soaking for 3 hours—drying at 70°C—crushing—passing through a 40-mesh sieve.

[0075] Bagasse 3: bagasse—in 2% NaOH by weight concentration, soaking for 2 hours—drying at 70° C.—crushing—passing through a 40-mesh sieve.

[0076] The "modified M9 medium" with bagasse 1, 2, and 3 as the sole carbon source was used as the fermentation medium, and the fermentation conditions were the same as in Example 1.

[0077] After culturing for 1 day, 2 days, 4 days, and 6 days, the following conclusions were drawn by counting:

[0078]

[0079] It can be seen that the acid trea...

Embodiment 5

[0081] Cellulase Qualitative Test

[0082] Solid medium: Na 2 HPO 4 0.6%, KH 2 PO 4 0.3%, NH 4 Cl 0.1%, NaCl 0.05%, sodium carboxymethyl cellulose 1%, agar 2%, balance: distilled water.

[0083] Seed medium: Na 2 HPO 4 0.6%, KH 2 PO 4 0.3%, NH 4 Cl 0.1%, NaCl 0.05%, bagasse 0.5%, balance: distilled water.

[0084] Seed fermentation condition is identical with embodiment 1. After two days of seed fermentation, take 0.1ml of the seed solution and dilute it with an appropriate gradient and spread it on a plate. Place the plate in an incubator at 37°C and cultivate it for a week. After a week of cultivation, the colony is about 1.5mm in size. Add 0.1% Congo red to the plate and stain it with 1 equivalent of NaCl for half an hour. The result of decolorization for 1 minute is shown in Figure 2, and a clear transparent circle appears. Because Congo red can combine with carboxymethyl cellulose, the flat panel appears red. If the bacteria can degrade cellulose, a transpa...

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PUM

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Abstract

The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.

Description

technical field [0001] The invention relates to a kind of human pale bacillus (Ochrobactrum anthropi) and the application of the strain in degrading plant stalks or preparing important enzymes. Background technique [0002] Plant fiber, as an important resource of natural fiber that exists in large quantities, has received extensive attention in its regeneration and utilization research. Due to the many disadvantages of using physical or chemical methods to treat bagasse, many experts have focused their attention on microbial treatment. Although there are many microorganisms that can utilize cellulose and hemicellulose, and many microorganisms can also utilize lignin, they are relatively difficult. Such as the strains disclosed in the Trichoderma reesei patent and the strains reported in the literature of Trichoderma viride, a significant defect of it is that the straw is difficult to degrade under normal conditions, mainly because the cellulose, hemicellulose in the straw ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/24C12N9/42
Inventor 童望宇魏东芝刘晓玲
Owner EAST CHINA UNIV OF SCI & TECH
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