59 results about "Sebacina" patented technology

The invention relates to the biological treatment for oil extraction wastewater, which in detail relates to a bacterial agent for treating thick oil wastewater and the method for preparing the same. The comprised components and their proportion by weight are as follows: pseudomonas aeruginosa 5-10%, bacillus subtilis 10-15%, lichen bacilli 5- 15%, Citrobacter propionate tumefaciens 10- 15%, liquid gold tumefaciens 10- 20%, annular gemma bacillus 5- 10%, wilting Bacillus pumilus 5- 10%, spherical arthrobacter 5- 10%, crabstick tumefaciens 5- 10%, and hot ground anaerobic rod baceria 10- 15%. It is prepared by activating bacteria, shake-flask culturing, expanding propagating and mixing. The cooperation action is strong, the biological surface activating agent generating- bacteria can enlarge the dissolution of hydrocarbon petroleum in water and make it is easier for hydrocarbon petroleum to contact with bacteria; the anaerobic bacteria can improve the biodegradation of thick oil wastewater; the degradation perfomace of aerobic bacteria is good and suitable for biological treatment for thick oil wastewater.

The invention relates to a rehabilitation technology of polycyclic aromatic hydrocarbon pollutants (PAHs), particularly discloses a mixed inoculant for degrading PAHs. The mixed inoculant comprises indigenous mixed bacteria, wherein the indigenous mixed bacteria comprise Pseudomonas sp. B08, Pseudomonas sp. BM, Bacillus sp. BYB, Bacillus sp. B07, Ochrobactrum sp. ZHL4, Gordonia sp. BGDS and Mycobacterium sp. BFZG. The mixed inoculant disclosed herein can be added according to an inoculation quantity of 5-10%, and when the initial concentration of PAHs is 180.67mgL<-1>, the total degradation rate of PAHs after being processed for 9 days can reach 91.21%.

The invention belongs to the technical field of agricultural microbiology, and relates to the fields of biochemistry and molecular biology simultaneously. Through separation, a bacillus amyloliquefaciens strain CH1 for producing a growth-promoting fungus apoptosis lipopeptide is obtained, which is preserved in the China Center for Type Culture Collection (CCTCC) with the preserving number of CCTCC: M208127. A bioactive substance is obtained from a culture of the bacillus amyloliquefaciens strain CH1 and is characterized as the growth-promoting fungus apoptosis lipopeptide. The molecular weight of the lipopeptide is 1,065 or a homologue thereof. The lipopeptide has an infrared spectrum as shown in Figure 6 in the instruction and consists of 9 carbon alkyls and 8 amino acids, wherein the seventh alkyl carbon and the last amino acid form a cyclic peptide through an oxygen atom. The bacillus amyloliquefaciens strain and the lipopeptide prepared from the same are tested on the biological application prospect in apoptosis.

The invention discloses a preparation method of Bacillus licheniformis (Bacilluslicheniformis subsp. Licheniformis, BL KN-08, CCTCC: NO. M208251) raw pesticide, comprising the following steps: A. screening B1 strains, collecting samples from soil and water body, adding sterile water, and standing; B. conforming the B1 strain the 16 SrDNA of which totally measures 1514 effective basic groups, and identifying; C. for B1 culture medium, adopting the response surface experiment designed by Box-Behken, taking the amount of spores as a screening index, and using a single factor experiment to select factors from multiple carbon sources, nitrogen sources and microelement factors; D. improving ferment biomass, and using the culture medium of the response surface method for batch fermentation; E. extracting and recovering spores; and F. detecting the amount of live spores. The invention can accurately detect the amount of the effective live spore in a product, has favourable repeatability, low detection cost, simple operation and convenient popularization and can simultaneously detect 7-8 samples; the amount of the live spores in the Bacillus licheniformis raw pesticide of the invention can reach 500 billion / g.

The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.

The invention relates to the technical field of bamboo artware processing, and discloses a method for mould and moth preventing for a bamboo product. The method comprises the following steps that firstly, newly-cut bamboo is wrapped by sludge in a pond, the sludge contains abundant nutrients suitable for growth of parasites, meanwhile, the viscous force is very large, the sludge has very strong adsorption force, disease pests and mould hidden in the bamboo are wrapped and adsorbed, and substances, causing insect damage and mould, in a bamboo body are transferred out; secondly, fumigated bamboo is placed in alums water to be immersed, alums has a very good restraining function on various gram negative and positive cocci and bacilli, Chinese herbal medicine liquid is used for fumigation after immersing, and it can be guaranteed that the bamboo product is not damaged by mould or moths for a long term through essential oil generated by the Chinese herbal medicine liquid; and thirdly, waterproof varnish is applied to the processed bamboo product, and the longer service life can be kept.

The invention relates to a Xanthobacter and its snail killer. A snail bacterium, namely Xanthobacter autotrophicus is a Xanthobacter autotrophicus II whose collection unit. It uses beef peptone agar plate or slope culture medium to separate bacterial strains, obtains snail-killing Xanthobacter autotrophicus solution (a hundred million grades / ml) by bottle-shaking fermentation process; and the application of Xanthobacter autotrophicus as a snail killer is that: making ultrasonic processing on the Xanthobacter autotrophicus solution and plant material forming agent, then mixing them to prepare a novel biological snail killer, which has the characters: (1) quickly killing the snail, and effectively preventing the snails from climbing up to flee; (2) harmless to non-target living things and fish, fast to naturally degrade and no pollution; (3) no toxic and side effect, and time of maintaining snail-killing pesticide effect 15 days long; (4) convenient to store, transport and use; etc.

The invention discloses a compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application of the compound microbial active filling material. Ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1 related in the invention are isolated bacteria obtained in a reactor for processing repugnant substances, such as H2S, in a water pollution control laboratory of the ecological environment research centre of the Chinese academy of sciences. The bacterial strains are preserved in the general microbial centre of the Chinese general microbial strain preservation and management committee. The preservation numbers are CGMCC No.7400 and CGMCC No.7399 respectively. The compound microbial active filling material disclosed by the invention has the advantages of being large in specific area, high in porosity, good in air permeability, low in resistance, high in bacterial cell loading capacity and less prone to run away; and because of being loaded with ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1, the compound microbial active filling material is difficult to decay and deform in a bioreactor for purifying waste gas, and therefore, the compound microbial active filling material can be used for a long time and is applied to complex processing conditions.

The invention belongs to the technical field of microbial denitrogenation, and particularly discloses an ochrobactrum anthropi FX02 strain and application thereof to wastewater denitrification. The strain is preserved in Guangdong Microbiological Culture Collection Center (GDMCC) on August 17, 2016 with the preservation number of GDMCC No:60062. The strain is a heterotrophic nitrification-aerobic denitrification bacteria, nitrification and denitrification can be conducted simultaneously, and the strain has high application value in sewage treatment.

Disclosed is a model human-source flora litter model construction and method for detecting flora in litter intestinal tract. The construction steps contain: (1) prepare healthy people fecal bacteria suspension; (2) paunch to farrowing sow to fetch litters; (3) breed the litters in 24-hour aero-ultra filter positive-pressure draft isolation room; (4) inoculate. The detecting steps contain: (1) extract the fecal microbe genome DNA; (2) analyze ERIC-PCR finger spectrum and community DNA crossing; (3) planting analyze bacillus in HFA litter intestinal tract flora; (4) planting analyze HFA bacteroide in HFA litter tract flora. This invention successfully plant human donator's bacillus and bacteroide in litter model intestinal tract. The finger spectrums comparing shows that the flora finger spectrum in litter model intestinal tract has homologous height with that of the human donator. This invention can be used to study the cross-correlation between intestinal tract flora and its host physiology and immune specificity, also it can be used to evaluate regulation and metabolism effect of functional foods or drugs to intestinal tract flora.

The invention discloses an agrobacterium sp. T29 capable of realizing high efficiency activating of mineral elements and heavy metal cadmium. The agrobacterium sp. T29 is preserved at Guangdong culture collection center (GDMCC) on 29th, Jan., 2018, and the preservation number is GDMCC No:60321. The agrobacterium sp. T29 is capable of realizing high efficiency activating of mineral elements potassium and phosphor and heavy metal cadmium. The agrobacterium sp. T29 is used for root activating of soil mineral nutrients and promoting plant growth, and is capable of dissolving soil cadmium effectively, and increasing hyperaccumulator plant soil heavy metal cadmium repairing efficiency. In soil heavy metal cadmium activating process using the agrobacterium sp. T29, no pollutant harmful for the environment is discharged, and the application prospect in the field of soil improvement is promising.

The invention discloses a Duganella bacterium and its application. The Duganella bacterium is sp. B2 CGMCC No:2056. Culturing Duganella sp. B2 CGMCC No:2056 will get purple bacteriocin. The fermenting condition for the bacterial strain is: culturing 24-60 h under 4-28 DEG C. The fermenting culture medium contains in each liter: starch 10-20 g, ferrisulphas 0.01-0.05 g, potassium nitrate 0.8-1.2 g, dipotassium hydrogen phosphate 0.5-1 g, and magnesium sulfate 0.5-1 g. The pH value of the fermenting culture medium is 6-9. The purple bacteriocin from the bacterial strain is a natural product, and free from any hidden harm due to artificial pigment. Tests show that the purple bacteriocin is of good biologic activity, will generate no side effect on animal bodies, and can be widely used in such fields as medicine and industries, etc.

The invention discloses a deodorant complex microbial inoculant and a preparation method and application thereof. The deodorant complex microbial inoculant is prepared from 4.8-10*10<8> cfu / mL of lactic acid bacteria, 2-5*10<6> cfu / mL of mucor, 3.9-7.2*10<7> cfu / mL of saccharomycetes, 3-10*10<6> cfu / mL of bacillus megatherium and 7-10*10<6> cfu / mL of bacillus subtilis, and the total count is 5.31-10.97*10<8> cfu / mL. The deodorant complex microbial inoculant is prepared from various microbial strains through fermentation, the deodorant complex microbial inoculant can grow well in feces due to the characteristics of flora composition, metabolic types, respiration types and functional diversity of effects, the deodorant complex microbial inoculant can grow well in feces and fast occupy favorable ecological niches, and therefore growth and propagation of putrefying bacteria producing repugnant substances can be effectively inhibited or the repugnant substances are directly degraded, repugnant odor in-situ source control is achieved, and the deodorization effect is achieved.

The invention relates to the technical field of microorganism and environment treatment, and particularly discloses an ochrobacterum sp. MGJ11, which is deposited with the CGMCC at Sep 16th, 2015 and is assigned the accession number CGMCC No.11385. The invention also discloses a method of immobilizing heavy metal cadmium in soil with the ochrobacterum sp. MGJ11, wherein the method particularly includes the steps of applying the ochrobacterum sp. MGJ11 to rhizosphere of plants, or mixing powder of the ochrobacterum sp. MGJ11 with seeds and planting the seeds, wherein the concentration of the inoculated microorganism is not less than 10<9> bacterial cells in every plant.

The invention belongs to the field of biochemical engineering and processes, relates to a method for adsorbing metal ions by a biological adsorbent and in particular relates to an ochrobactrum intermedium extracellular polysaccharide and application of the ochrobactrum intermedium extracellular polysaccharide to the aspect of soil improvement. The method comprises the following steps: step (1): obtaining ochrobactrum intermedium capable of producing an extracellular polysaccharide; step (2): obtaining the ochrobactrum intermedium extracellular polysaccharide; step (3): adsorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide; step (4): desorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide.

The invention discloses a special higher-utilization-ratio environment-friendly coated fertilizer for blueberries. The invention is characterized in that the fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of rhizobia, 1-2 parts of Azotobacter chroococcum, 1-3 parts of Bacillus megatherium, 34-37 parts of soybean meal, 30-34 parts of rape seed cake, 45-50 parts of clover stover, 1-2 parts of borax, 3-5 parts of bentonite, 7-11 parts of sodium humate, 4-6 parts of castor oil, 3-5 parts of diglycol, 2-5 parts of vitrified microbead, 14-20 parts of hydroxypropyl methyl cellulose, 4-7 parts of gypsum, 7-9 parts of effective microorganisms and 9-12 parts of soil conditioner. The fertilizer solves the problem of low utilization ratio of nitrogen; the nitrogen fixation fungus added in the fertilizer can reduce the application amount of the nitrogen fertilizer, and lowers the ecological environment for land pollution protection; proper proportions of phosphate fertilizer and potassium fertilizer are added according to the fertilizer demand characteristics of blueberries to satisfy the nutrients required in the whole blueberry growth period; the coating technique is utilized to enable the fertilizer to release nutrients slowly for a long time and implement balanced wants and needs of the blueberries, thereby avoiding the phenomenon of seedling burning due to overfertilization; and the fertilizer can promote blossoming and fruiting of the blueberries and enable the blueberries to come into the market in advance.

The invention discloses an efficient phosphate solubilizing bacillus megaterium in a tree rhizosphere. The preservation number is CCTCC NO. M 2017454, and the efficient phosphate solubilizing bacillusmegaterium is named as Bacillus megaterium L205. The test shows that the phosphate solubilizing amount of bacillus megaterium reaches 203.04mg / L, and the liquid bacterium can effectively solubilize insoluble inorganic phosphorus, increase the content of available phosphorus in red-yellow soil, and promote the growth of tree seedlings. The efficient phosphate solubilizing bacillus megaterium provided by the invention enriches the sink population of bacillus megaterium at the culture collection center and provides convenience for better exploitation of microbial population resources of bacillusmegaterium. Meanwhile, the bacillus megaterium strain L205 can secrete organic acids, solubilize inorganic phosphorus and improve the phosphorus absorption and utilization of plants, is especially suitable for the production of tree biological fertilizers and tree growth promoting bacteria in red-yellow soil areas in South China, and can be used for further developing compound microbial fertilizers and bacteria.

The invention discloses a preparation method of bacillus licheniformis raw powder with 100 billion live spores per gram. The method comprises the following steps of: (A) screening a fermentation culture medium of a strain BL KN-08, designing an experiment by adopting a response surface method invented by Box-Behken, and using a spore number as a screening index to obtain a culture medium formula beneficial to improving the spore number; (B) increasing the fermentation biomass, carrying out batch fermentation through the culture medium formula screened by using the response surface method to determine an optimal fermentation process beneficial to generating spores, and further increasing the spore number through fed-batch fermentation; and (C) extracting and recovering the spores. The bacillus licheniformis raw powder prepared by using the process has the advantages of clean and safe production, high recovery rate, stable product spore number, and the like, and the live spore number of the powder reaches 100 billions per gram.

The invention designs compound separated and extracted by marine fusarium (Fusarium sp. QY010) and application thereof. The compound is the active compound which is obtained by fermenting, separating and purifying the fusarium QY010, wherein the chemical name of the active compound is 5-butyl pyridine formic acid, and the active compound is obtained by screening the pseudomonas aeruginosa screening model QSIS2 and the chromobacterium type bacterial strain CV026. Within the concentration range that the pathogenic bacteria can not be killed, the compound has the double-inhibitory action to the quorum-sensing system of the pseudomonas aeruginosa and the chromobacterium, can restrain the pathogenic behavior of the bacterial quorum sensing regulation, can obviously reduce the yield of the chromobacterium and the mycetin, can reduce the virulence factors of the quorum-sensing regulation of the pseudomonas aeruginosa, and can weaken the drug resistance of the pathogenic bacteria. The invention has the advantages of being easy to obtain the preparation raw materials, easy to ferment and cultivate the bacterial strain, high in yield, and simple and easy to control the technical process for preparing the compound. Furthermore, the analogues derived by the compound can be used for the research and the development of novel antibacterial agents.

The invention provides a bacteria-algae complex agent for treating coal chemical industry wastewater and a preparation method and application thereof. The bacteria-algae complex agent comprises the following components in parts by weight: 9-13 parts of pseudomonas, 3-6 parts of ochrobactrum, 3-6 parts of flavobacterium and 15-25 parts of algae. By adopting bacteria-algae complex agent provided by the invention, the coal chemical industry wastewater can be effectively and specifically purified.

The invention discloses a bare-glass fly arthrobacter strain, which has the collection number of CGMCC No.3554 and has 16S rRNA genes shown as ID No:1. A method for producing feeding proteins by using the bare-glass fly arthrobacter strain to decompose gossypol, which comprises the following steps of: a, preparing seeds; and b, inoculating the seeds into a cottonseed cake dreg culture medium, in which glucose and corn steep liquor are added, for growth, and fermenting the seeds for 72 hours at 37 DEG C to prepare the fermented and detoxicated cottonseed cake dreg feeding proteins. A fermentation culture medium of the bare-glass fly arthrobacter strain has low production cost; the method for producing the feeding proteins by using the bare-glass fly arthrobacter strain to ferment and decompose the gossypol in the cottonseed cake dregs has simple process; and after the cottonseed cake dregs are fermented and detoxicated, free gossypol content is reduced by 60 percent, and nutritive values of cotton dreg substrates are improved.

The present invention is to provide a starter to be used for producing fermented foods and fermented beverages with a simple means, as a fermentation starter excellent in storage properties and stability. A fermentation starter excellent in storage properties and stability is produced by treating the surface of a solid starter comprising cereal starch materials such as oats and the like containing fermentation microorganisms with dry-heat treatment such as roasting for a short time. According to the method, microorganisms on the surface are sterilized by forming a layer treated with dry-heat treatment such as roasting on the surface of a solid fermentation starter, and the invasion of external microorganisms is prevented with the layer, and further, internal yeast and other bacilli can be stored with maintaining their activity by being protected by the treated layer.

The invention provides a method for culturing bacteria and application thereof and relates to a method for culturing microorganisms with low price and application thereof to high-efficiency reduction and fixation of heavy metal. In the method for culturing the bacteria by using a low-price culture medium, bean curd water comprises rich carbon sources and nitrogen sources and low-price sugar dreg lixivium is added, human ochrobactrum lupini grows well when the pH value is 7.5, and escherichia coli, bacillus subtilis and bacillus cereus can grow under the conditions, so culture cost is greatly reduced. The human ochrobactrum lupini are further used for treating chromium-containing and nickel-containing industrial waste water in an optimized culture medium, and the result shows that the nickel and chromium reducing efficiency of the human ochrobactrum lupini is high.

The present invention provides an Arthrobacter protophormiae SCUEC5 strain, wherein the preservation number is CCTCC NO:M2014404, the nucleotide sequence of the strain is represented by SEQ ID NO.1, and the strain can be applied in nicotine degradation and can further be provided for accelerating tobacco leaf maturation. The present invention further provides a method for screening and separating the strain, wherein the method comprises: carrying out enrichment culture, carrying out separation purification, inoculating the separated and purified single colony into a nicotine culture medium, carrying out shaking culture for 1-3 days at a room temperature, determining the nicotine content in the fermentation broth of the nicotine culture medium, and re-screening the strain according to the nicotine degradation rate so as to obtain the Arthrobacter protophormiae SCUEC5 strain.

The invention discloses a bacterial strain for low-temperature glycolysis of corn straw as well as a fermentation culture method and application of the bacterial strain. The invention firstly discloses a separated short bacillus strain, the microbial preservation number of which is: CGMCC (China General Microbiological Culture Collection Center) No.15177. The invention optimizes relevant parameters of a fermentation preparation method of the separated short bacillus strain. The short bacillus separated by the invention can grow at different temperatures and still has exuberant growing power under the low-temperature condition of 4 DEG C. The strain separated by the invention can be used for high efficient glycolysis of the corn straw, and can be separately applied to glycolysis of the straw or in combination with EM bacteria. Fermentation broth of the strain disclosed by the invention is mixed with EM bacterial liquid and then the low-temperature glycolysis of the corn straw is performed by a mixture, so that the glycolysis effect of the straw is significantly improved and the glycolysis rate is increased by 47.4 percent. The separated short bacillus strain disclosed by the invention has a wide application prospect in preparing a straw-decomposing inoculant, in particular to a corn straw-decomposing inoculant.

The invention discloses a method for producing rhamnolipid by virtue of fermentation and separation of pseudomonas aeruginosa. The method comprises the following steps: 1), transferring pseudomonas aeruginosa strains into a shake flask by using an inclined plane inoculation method to obtain a pseudomonas aeruginosa seed liquid; 2), adding a fermentation medium into a fermentation tank for sterilization, inoculating the pseudomonas aeruginosa seed liquid in the fermentation tank for fermentation according to 8%-12% of inoculation concentration, and fermenting the pseudomonas aeruginosa seed liquid for 120 hours to obtain pasty fermentation broth, wherein 0.1-0.5% glycerophosphorylethanolamine is added before the seed liquid is inoculated; and 3) carrying out twice extraction and distillation on the pasty fermentation broth to obtain paste rhamnolipid with the purity of 80%-90%. The method can meet the low-cast large-scale industrialized production requirements of rhamnolipid with the purity of more than 80%.

The invention relates to an antagonistic bacterium strain capable of being used for prevention and control of a plant root cancer. The classification name of the antagonistic bacterium is ochrobactrum oryzae which was preserved in China General Microbiological Culture Collection Center on September 2, 2014 with the preservation number of CGMCC No. 9634. The strain is used for prevention and control of the root cancer caused by infection of A. tumefaciens to a plant and biological treatment of soil infected by the A. tumefaciens.

The invention belongs to the technical field of biology, and relates to a bacterial strain for high-producing bacterial polysaccharide and a method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste. The bacterial strain is preserved in the China Center for Type Culture Collection (CCTCC); the preservation number is CCTCC M 2016273. A bacterial strain of intermediate ochrobactrum ZY03 is inoculated in a culture medium with a sucrose content of 10g / L; the yield of the polysaccharide can reach 6g / L at most; the conversion ratio can reach 67 percent at most.

The invention discloses a short bacillus and an application thereof. The short bacillus is a curtobacterium sp. SY311 bacterial strain and is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.7181. The method for preparing trehalose by fermenting the short bacillus concretely comprises the following steps of: (1) activating the strain; (2) preparing a seed solution; (3) fermenting; (4) deproteinizing; (5) collecting a trehalose component; and (6) carrying out posttreatment, namely carrying out decoloration, filtering, low pressure concentration, crystallization and drying on the trehalose component, so that the trehalose product is obtained. According to the invention, the adopted strain is mutated by ultraviolet, trehalose enzymatic activity is low, and accumulative increase of trehalose in fermentation liquor can be facilitated, so that the yield of the final trehalose product is increased; and the preparation technology disclosed by the invention is simple, trehalose is directly synthesized by adopting a one-step method, raw materials are available, cost is low, less impurities are contained in the trehalose product, purification is easy, and the method provided by the invention is applicable to industrial production.

The invention discloses a microbial product for treating heavy metal polluted saline-alkali soil. The microbial product comprises a culture medium and a target microorganism, wherein the culture medium contains a nutrition medium and nutrition liquid, and the formula of the nutrition liquid is as follows: each 1L of water contains 10g-20g of agar, 0.2g-0.7g of KH2PO4, 2.5g-3.0g of (NH4)2SO4, 0.5g-1.5g of CaSO4, 1.5g-2.5g of MgSO4.7H2O, 3.0g-4.0g of lactic acid, 0.5g-1.5g of yeast powder, 0.05g-0.15g of ascorbic acid, 0.05g-0.15g of mercaptoacetic acid and 0.2g-0.7g of FeSO4.7H2O; and the target microorganism contains pseudochrobactrum asaccharolyticum, rhodobacter sphaeroides, micrococcus flavus PS5 and white-rot fungi. Lead and zinc ions are enriched and passivated in cells by biologicalmetabolism of microorganisms, protein organisms can be produced and can be combined with adsorbed metal ions, so that the degradation efficiency is improved; and the microbial product has good environmental adaptive capacities such as temperature and pH, and a relatively good remediation effect is achieved.