Bacteria-algae complex agent for treating coal chemical industry wastewater and preparation method thereof
A technology of coal chemical wastewater and compounding agent, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of secondary pollution, poor biodegradability, difficult-to-treat wastewater, etc., and achieve the promotion of substances Cycle, strong impact resistance, avoid secondary pollution effect
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Embodiment 1
[0043] (1) Add agar at 1.8% to a certain coal chemical industry wastewater to make a solid medium. The initial water quality of the secondary effluent of coal chemical wastewater is: pH 8.82, COD concentration 1200mg / L, NH 3 -N concentration is 105mg / L, volatile phenol concentration is about 100mg / L. The dilution of activated sludge was inoculated into solid medium to screen out three functional bacteria: Pseudomonas putida, Ochrobactrumcytisi, and Flavobacterium soli; The three strains were inoculated into the liquid culture medium and cultured respectively. After the strains were cultivated to a dry weight of 2g / L, they were mixed to obtain a mixed bacterial liquid. The mass ratios of the three strains were 10%, 5%, and 5%, respectively.
[0044] (2) Cultivate Chlorella in the culture solution, stop the cultivation when the dry weight of the algae concentration in the culture solution is 2g / L, save it, and obtain the Chlorella sp. algae solution.
[0045] (3) Implant the m...
Embodiment 2
[0050] (1) Add agar to a certain coal chemical industry wastewater with a mass ratio of 1.5% to make a solid medium. The initial water quality of coal chemical wastewater is: pH 10.50, COD concentration 4000mg / L, NH 3 -N concentration is 300mg / L, volatile phenol concentration is 400mg / L, TP concentration is about 23.1mg / L. The dilution of activated sludge was inoculated into the medium, and three functional bacteria were screened out: Pseudomonas putida, Ochrobactrum cytisi, and Flavobacterium terrae. The three bacterial strains were inoculated into liquid culture medium for culture respectively, and after the bacterial strains were cultivated to a dry weight of 3g / L, they were mixed at a mass ratio of 12%, 4%, and 4% respectively to prepare a mixed bacterial liquid.
[0051] (2) Cultivate Scenedesmus (Scenedesmus) in the culture solution, stop the cultivation when the dry weight of the algae concentration in the culture solution is 2 g / L, store it, and obtain the Scenedesmus...
Embodiment 3
[0057] (1) Take wastewater from a coal gasification plant in China and add agar at 2.0% to make a solid medium. The initial water quality of coal chemical wastewater is: COD is 1000-2500mg / L, BOD5 (biochemical oxygen demand for five days) / CODCr (chemical oxygen demand) is 0.28-0.35, pH is 8-9, and total phenols are 50mg / L L, NH 3 -N 80mg / L. Before the treatment, the pH value of the sewage was first adjusted to 7, and then three functional bacteria were screened out: Pseudomonas putida, Ochrobactrum anthropi, and Flavobacterium terrae; The strains were inoculated into the liquid culture medium for cultivation, and the strains were cultured to a dry weight of 3g / L, and then mixed. The mass ratios of the three strains were 13%, 4%, and 3%, respectively.
[0058] (2) Cultivate Chlorella sp. (Chlorella sp.) and Scenedesmus (Scenedesmus) in nutrient solution, stop cultivating when the algae concentration dry weight value in the nutrient solution is 2g / L, preserve, obtain Chlorella...
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