Preparation method of Bacillus licheniformis and raw pesticide
A technology of Bacillus licheniformis and spores, which is applied in the field of preparation of Bacillus licheniformis and original medicine, can solve the problems of being susceptible to contamination by miscellaneous bacteria, extensive management, unstable product quality, etc.
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[0048] A method for preparing a former drug from bacillus licheniformis, the steps are:
[0049] 1. Screening of B1 strains:
[0050] Collect samples from different soils and water bodies across the country, weigh 1.0g of the sample into a small triangle flask on a sterile ultra-clean bench, add 9mL of sterile water, shake it fully and let it stand, take the supernatant as a concentration gradient Dilution (1×10 -4 , 1×10 -5 ,, 1×10 -6 , 1×10 -7 , 1×10 -8 ). Use a pipette gun to draw 100 ul of the diluent and evenly spread it on the separation medium. After culturing in a temperature-controlled incubator at 30°C for 3-5 days, pick out the strains with high spore synchronization rate (spore formation rate ≥ 90%), and then test Microscopic examination after Lang's staining, the bacterial strain with Bl morphological characteristics was selected and transferred to B 1 In the liquid shake flask fermentation medium, the filling volume of 500mL shake flask is 25mL, the shaker...
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