Bacterial strain for low-temperature glycolysis of corn straw as well as fermentation culture method and application of bacterial strain
A technology of fermentation culture and fermentation medium, which is applied in the field of separation and application of straw decomposing bacteria in cold regions, can solve the problems of low ambient temperature of straw, inconvenient transportation, environmental pollution, etc., and achieve improved straw fermentation effect and fermentation rate , the effect of strong cold resistance
Active Publication Date: 2018-11-23
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology
However, the utilization rate of straw in China is less than 33%, mainly because straw is difficult to collect in the field, and transportation is inconvenient, and most of them are burned or discarded.
Heilongjiang Province is a major agricultural province in China. It produces a huge amount of straw every year. Due to the low ambient temperature, low return conditions and poor promotion of these straws, many straws are burned or discarded, which not only wastes valuable crop resources, but also causes huge damage to the environment. Pollution
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Embodiment 1
[0036] Example 1 Isolation and identification of straw-decomposing bacteria in cold regions
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Abstract
The invention discloses a bacterial strain for low-temperature glycolysis of corn straw as well as a fermentation culture method and application of the bacterial strain. The invention firstly discloses a separated short bacillus strain, the microbial preservation number of which is: CGMCC (China General Microbiological Culture Collection Center) No.15177. The invention optimizes relevant parameters of a fermentation preparation method of the separated short bacillus strain. The short bacillus separated by the invention can grow at different temperatures and still has exuberant growing power under the low-temperature condition of 4 DEG C. The strain separated by the invention can be used for high efficient glycolysis of the corn straw, and can be separately applied to glycolysis of the straw or in combination with EM bacteria. Fermentation broth of the strain disclosed by the invention is mixed with EM bacterial liquid and then the low-temperature glycolysis of the corn straw is performed by a mixture, so that the glycolysis effect of the straw is significantly improved and the glycolysis rate is increased by 47.4 percent. The separated short bacillus strain disclosed by the invention has a wide application prospect in preparing a straw-decomposing inoculant, in particular to a corn straw-decomposing inoculant.
Description
technical field [0001] The present invention relates to an isolated cold region corn stalk decomposing bacterial strain, and also relates to the application of the cold region straw decomposing bacterial strain in high-efficiency fermentation of corn stalks at low temperature, belonging to the field of separation and application of cold region straw decomposing bacteria. Background technique [0002] Crop straw is rich in nutrients such as cellulose, hemicellulose, lignin, protein and mineral elements, and is a renewable biological resource. However, the utilization rate of straw in China is less than 33%, mainly because straw is difficult to collect and transport in the field, and most of them are burned or discarded. Heilongjiang Province is a major agricultural province in China. It produces a huge amount of straw every year. Due to the low ambient temperature, low return conditions and poor promotion of these straws, many straws are burned or discarded, which not only wa...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C05F11/08C12R1/07
CPCC05F11/08C12N1/20C05F17/20C12N1/205C12R2001/07Y02W30/40
Inventor 李凤兰徐媛媛徐永清冯艳忠杨秀梅袁强王丽娟
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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