Bacterial strain for high-producing bacterial polysaccharide and method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste

A bacterial polysaccharide and waste technology, applied in the field of P. intermedia exopolysaccharide and biosorbent adsorption of metal ions, can solve the problem of less research on bacterial polysaccharides, and achieve the effect of strong adsorption capacity of metal ions and great application value

Active Publication Date: 2017-05-31
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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AI Technical Summary

Problems solved by technology

At present, the research on the activity of bacterial polysaccharides is mainly concentrated in the fields of medicine, food, and chemical industry, and the research on bacterial polysaccharides in agricultural soils is still relatively small.

Method used

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  • Bacterial strain for high-producing bacterial polysaccharide and method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste
  • Bacterial strain for high-producing bacterial polysaccharide and method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste
  • Bacterial strain for high-producing bacterial polysaccharide and method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening and Species Identification of Exopolysaccharide Strains of Pallidum intermedium

[0030] The strain separation carbon is from the moldy astragalus dregs, and the microorganisms with nitrilase activity are obtained by screening and separating from the astragalus dregs. Liquid medium composition: peptone 8g / L, yeast powder 5g / L, sodium chloride 10g / L, pH 7.0; plate medium composition: peptone 8g / L, yeast powder 4g / L, sodium chloride 10g / L , agar powder 15g / L; screening medium composition: glycerol 3g / L, magnesium chloride 0.4g / L, magnesium sulfate 0.5g / L, sodium hydrogen phosphite 2g / L, potassium hydrogen phosphite 2g / L, after sterilization Add 5 mL / L of β-aminopropionitrile. Dilute different gradients of the culture medium that grows bacteria on the screening medium, spread on the plate, select different single colonies and inoculate them into the screening medium for 72 hours of cultivation, centrifuge to obtain the bacteria, take 0.5g of bacteria and centrif...

Embodiment 2

[0032] Fermentation of Pallidum intermedia ZY03 and preparation of resting cells

[0033] Inoculate Pallidum intermedium ZY03 in potato culture medium, culture in a medium with a temperature of 30°C, a rotation speed of 180rpm, and a ventilation ratio of 1:1 for 18-24 hours to obtain a seed culture solution; the seed culture solution is inoculated into a polysaccharide fermentation medium Among them, the pH of the polysaccharide fermentation medium is natural, and the components are as follows in terms of mass percentage: 20% of potato juice, (NH 4 ) 2 SO 4 0.8%, Glucose 2%, CaCl 2 0.02%, MgSO 4 0.01%; centrifuge at 10000rpm for 15 minutes to collect the bacterial cells, wash with normal saline for 1-2 times, and obtain resting cells of P. intermedium ZY03.

Embodiment 3

[0035] Filter the thalline cell fermentation broth in Example 2 to obtain wet thallus, place them in 4 identical polysaccharide fermentation mediums respectively, the thalline mass concentration is 5%, and ferment 2, 3, 4, 5 days; the fermentation broth was removed by high-speed centrifugation to remove bacteria, the supernatant was vacuum concentrated to 1 / 3 of the volume of the original solution, precipitated with 2 times the volume of absolute ethanol, and the precipitate was collected by centrifugation. The precipitate was deproteinized by the Sevag method, and then ethanol For precipitation, the precipitate was washed 2 to 3 times with an equal amount of 75% ethanol, and then dried to obtain the extracellular crude polysaccharide product. The polysaccharide content was determined by the phenol-concentrated sulfuric acid method in Table 1.

[0036] Table 1 The content of bacterial polysaccharides after different days of fermentation

[0037]

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Abstract

The invention belongs to the technical field of biology, and relates to a bacterial strain for high-producing bacterial polysaccharide and a method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste. The bacterial strain is preserved in the China Center for Type Culture Collection (CCTCC); the preservation number is CCTCC M 2016273. A bacterial strain of intermediate ochrobactrum ZY03 is inoculated in a culture medium with a sucrose content of 10g / L; the yield of the polysaccharide can reach 6g / L at most; the conversion ratio can reach 67 percent at most.

Description

technical field [0001] The invention belongs to the field of biochemical engineering and technology, and relates to a method for absorbing metal ions by a biosorbent, in particular to a P. intermedia exopolysaccharide and an application thereof. Background technique [0002] Polysaccharides are a class of unique biomacromolecules produced by organisms that widely exist in nature. It has a variety of special and in most cases quite complex chemical structures, different physiological functions and a wide range of (potential) applications. In recent years, as a new type of fermentation product, microbial polysaccharides have unique physical and chemical properties, and have been used as emulsifiers, thickeners, stabilizers, gelling agents, suspending agents and lubricants in petroleum, chemical, food, pharmaceutical, Environmental protection and cosmetics and other fields. Because polysaccharides, like hyaluronic acid, xanthan gum, thermal gel, gellan gum, and welan gum, exh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 马新飞张森段金廒郭盛陶伟伟肖平
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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