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Ochrobactrum intermedium extracellular polysaccharide and application of ochrobactrum intermedium extracellular polysaccharide to aspect of soil improvement

A technology of Bacillus intermedius and extracellular polysaccharide, which is applied in the field of biosorbent adsorption of metal ions and Bacillus intermedius exopolysaccharide, can solve the problem of less research on bacterial polysaccharides, and achieve the effect of strong ability to adsorb metal ions and great application value.

Active Publication Date: 2017-05-31
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the activity of bacterial polysaccharides is mainly concentrated in the fields of medicine, food, and chemical industry, and the research on bacterial polysaccharides in agricultural soils is still relatively small.

Method used

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  • Ochrobactrum intermedium extracellular polysaccharide and application of ochrobactrum intermedium extracellular polysaccharide to aspect of soil improvement
  • Ochrobactrum intermedium extracellular polysaccharide and application of ochrobactrum intermedium extracellular polysaccharide to aspect of soil improvement
  • Ochrobactrum intermedium extracellular polysaccharide and application of ochrobactrum intermedium extracellular polysaccharide to aspect of soil improvement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening and Species Identification of Exopolysaccharide Strains of Pallidum intermedium

[0030] The strain separation carbon is from the moldy astragalus dregs, and the microorganisms with nitrilase activity are obtained by screening and separating from the astragalus dregs. Liquid medium composition: peptone 8g / L, yeast powder 5g / L, sodium chloride 10g / L, pH 7.0; plate medium composition: peptone 8g / L, yeast powder 4g / L, sodium chloride 10g / L , agar powder 15g / L; screening medium composition: glycerol 3g / L, magnesium chloride 0.4g / L, magnesium sulfate 0.5g / L, sodium hydrogen phosphite 2g / L, potassium hydrogen phosphite 2g / L, after sterilization Add 5 mL / L of β-aminopropionitrile. Dilute different gradients of the culture medium that grows bacteria on the screening medium, spread on the plate, select different single colonies and inoculate them into the screening medium for 72 hours of cultivation, centrifuge to obtain the bacteria, take 0.5g of bacteria and centrif...

Embodiment 2

[0032] Fermentation of Pallidum intermedia ZY03 and preparation of resting cells

[0033] Inoculate Pallidum intermedium ZY03 in potato culture medium, culture in a medium with a temperature of 30°C, a rotation speed of 180rpm, and a ventilation ratio of 1:1 for 18-24 hours to obtain a seed culture solution; the seed culture solution is inoculated into a polysaccharide fermentation medium Among them, the pH of the polysaccharide fermentation medium is natural, and the components are as follows in terms of mass percentage: 20% of potato juice, (NH 4 ) 2 SO 4 0.8%, Glucose 2%, CaCl 2 0.02%, MgSO 4 0.01%; centrifuge at 10000rpm for 15 minutes to collect the bacterial cells, wash with normal saline for 1-2 times, and obtain resting cells of P. intermedium ZY03.

Embodiment 3

[0035] Filter the thalline cell fermentation broth in Example 2 to obtain wet thallus, place them in 4 identical polysaccharide fermentation mediums respectively, the thalline mass concentration is 5%, and ferment 2, 3, 4, 5 days; the fermentation broth was removed by high-speed centrifugation to remove bacteria, the supernatant was vacuum concentrated to 1 / 3 of the volume of the original solution, precipitated with 2 times the volume of absolute ethanol, and the precipitate was collected by centrifugation. The precipitate was deproteinized by the Sevag method, and then ethanol For precipitation, the precipitate was washed 2 to 3 times with an equal amount of 75% ethanol, and then dried to obtain the extracellular crude polysaccharide product. The polysaccharide content was determined by the phenol-concentrated sulfuric acid method in Table 1.

[0036] Table 1 The content of bacterial polysaccharides after different days of fermentation

[0037]

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Abstract

The invention belongs to the field of biochemical engineering and processes, relates to a method for adsorbing metal ions by a biological adsorbent and in particular relates to an ochrobactrum intermedium extracellular polysaccharide and application of the ochrobactrum intermedium extracellular polysaccharide to the aspect of soil improvement. The method comprises the following steps: step (1): obtaining ochrobactrum intermedium capable of producing an extracellular polysaccharide; step (2): obtaining the ochrobactrum intermedium extracellular polysaccharide; step (3): adsorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide; step (4): desorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide.

Description

technical field [0001] The invention belongs to the field of biochemical engineering and technology, and relates to a method for absorbing metal ions by a biosorbent, in particular to a P. intermedia exopolysaccharide and an application thereof. Background technique [0002] Polysaccharides are a class of unique biomacromolecules produced by organisms that widely exist in nature. It has a variety of special and in most cases quite complex chemical structures, different physiological functions and a wide range of (potential) applications. In recent years, as a new type of fermentation product, microbial polysaccharides have unique physical and chemical properties, and have been used as emulsifiers, thickeners, stabilizers, gelling agents, suspending agents and lubricants in petroleum, chemical, food, pharmaceutical, Environmental protection and cosmetics and other fields. Because polysaccharides, like hyaluronic acid, xanthan gum, thermal gel, gellan gum, and welan gum, exh...

Claims

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Application Information

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IPC IPC(8): C12P19/04C09K17/32B01J20/24C12R1/01
CPCB01J20/24B01J2220/4825C09K17/32C12P19/04
Inventor 张森段金廒陶伟伟严辉马新飞宿树兰
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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