41 results about "Nicotine degradation" patented technology

The invention discloses an Acinetobacter and application thereof. Production bacterial strain liquid is fermented and cultured to form corresponding preparation. The Production bacterial strain is the Acinetobacter (Acinetobacter sp.ND12); and the bacterial strain was preserved in China General Microbiological Culture Collection Center on November 5, 2009, and a preservation number is CGMCC No.3410. The bacterial strain has stronger nicotine degradation capacity and nicotine toxicity resistance capacity, and can be grown at the nicotine concentration of 3.5g / L; and by large-scale culturing of the bacterial strain, the pure nicotine can be degraded by using a whole cell of the bacterial strain as a biocatalyst, and meanwhile the content of the nicotine in tobacco is also reduced. The Acinetobacter and the application thereof have the advantages of capacities of adjusting the content of the nicotine in the tobacco raw material, improving availability of the tobacco, degrading the nicotine in smoke dust, reducing pollution of the nicotine to environment and the like.

The invention relates to a pseudomonas fluorescens preparation and application thereof, and belongs to the field of biotechnology. A preparation method of the pseudomonas fluorescens preparation comprises the following steps of 1, selecting pseudomonas fluorescens 1206 as a production bacterial strain, wherein the pseudomonas fluorescens 1206 has a preservation number of CGMCC NO.3149, 2, carrying out strain culture through inoculating a culture solution with the pseudomonas fluorescens 1206 subjected to inclined plane culture, wherein the culture solution comprises 3% of beef extract, 0.5% of peptone, 0.5% of sodium chloride and the balance distilled water, has a pH value of 7.0 to 7.2 and is put in a shaking bottle, and carrying out culture at a shaking bottle rotate speed of 150rpm at a temperature of 28 to 30 DEG C for 18 hours to obtain a liquid fermented strain, and 3, carrying out fermentation tank fermentation through inoculating a liquid fermentation system in a fermentation tank with the liquid fermented strain, wherein the liquid fermentation system comprises 35g / L of maltose, 4.03g / L of extract yeast, 4.72g / L of ammonium sulfate, 0.4g / L of K2HPO4, 0.075g / L of MgSO4.7H2O, 0.0005g / L of FeSO4.7H2O, 0.015g / L of CaCl2 and 0.1g / L of NaCl, and carrying out fermentation under the conditions of a fermentation temperature of 27 to 29 DEG C, an inoculation amount of 5 to 10%, a fermentation tank stirring speed of 120 to 150rpm, an aeration rate of 5 to 10% and fermentation time of 24 to 48 hours. The pseudomonas fluorescens preparation has a short fermentation period and a high nicotine degradation rate, can improve tobacco quality and can be utilized for degradation of nicotine of tobacco or tobacco waste.

The invention relates to a method of biology degradation nicotine which uses (Ochrobactrum intermedium) DN2 as bacteria seed and uses the nicotine zymosis enzyme of the Ochrobactrum intermedium DN2 or Ochrobactrum intermedium DN2 to effect on the flue-cured tobacco leaf, pipe tobacco, industrial or agricultural tobacco leftover bits and pieces and tobacco residues and the extracting liquid of the above materials to do degradation to part of or all of the nicotine.