Pseudomonas monteilii SCUEC2 strain, screening method and application thereof

A technology of Pseudomonas monsonii and strains, applied in the field of nicotine degradation, can solve the problems of tobacco leaves in the field that have not been confirmed by experiments, and achieve the effect of accelerated maturation and strong degradation ability

Inactive Publication Date: 2014-10-15
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0004] Chinese invention patent applications (application numbers: 200710013068.3, 200710013069.8, 200710070805.3, 200910097536.9, etc.), the bacterial strains involved in these patents have effective degradation performance on nicotine, and the only disadvantage is that nicotine degradation is carried out under indoor laboratory conditions. Whether real field tobacco leaves have any effect has not been confirmed experimentally

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  • Pseudomonas monteilii SCUEC2 strain, screening method and application thereof
  • Pseudomonas monteilii SCUEC2 strain, screening method and application thereof

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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0016] The Pseudomonas monteilii (Pseudomonas monteilii) SCUEC2 strain provided in this example was preserved in the China Center for Type Culture Collection on April 17, 2014, the address is Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M2014134, the 16S nucleotide sequence of the strain is shown in SEQ ID NO.1, including 968 bases.

[0017] The screening and isolation method of Pseudomonas monteilii SCUEC2 provided in this example is as follows: (1), enrichment culture: put 2 g of the soil in the tobacco planting field into 20 mL of nicotine medium, and set the temperature at 30° C. 180rpm shaker was used under the conditions for culture. After 48 hours of culture, 1mL of the enrichment solution was transferred to fresh nico...

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Abstract

The invention provides a Pseudomonas monteilii SCUEC2 strain, which has a preservation number of CCTCC NO:M2014134 and a nucleotide sequence shown as SEQ ID NO.1. The strain not only can be applied to nicotine degradation, but also can accelerate tobacco leaf ripening. The invention also provides a method for screening and separation of the strain. The method comprises the steps of: firstly conducting enrichment culture, then carrying out separation and purification, and finally inoculating the separated and purified strain into a nicotine medium by single colony, performing shake cultivation at room temperature for 1-3d, then measuring the content of nicotine in the fermentation broth of the nicotine medium, and re-screening the strain according to the nicotine degradation rate, thus obtaining the Pseudomonas monteilii SCUEC2 strain.

Description

technical field [0001] The invention relates to a microbial strain for degrading nicotine, in particular to a Pseudomonas monsonii SCUEC2 strain, a screening method and application of the strain, and belongs to the technical field of nicotine degradation. Background technique [0002] Nicotine is not only the most important chemical component present in tobacco, but the level of nicotine content plays a very important role in the quality of tobacco products. At present, the nicotine content of flue-cured tobacco in my country is generally high, especially in the upper tobacco leaves. For example, the appearance quality of tobacco leaves in some areas around Shennongjia is close to the domestic and international advanced level, but the nicotine content of tobacco leaves in some tobacco-producing counties is as high as 3.5% to 4.5% %, and the nicotine content in the upper part of Burley tobacco is as high as 6%. This not only affects the quality of the upper tobacco, but also ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20A01N63/00A01P21/00C12R1/38
Inventor 李晓华孔文郭利李阳杨振飞王晓丽何冬兰程国军刘涛李开曹丽君陈涛龚传清李永辉曹祥练
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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