36 results about "Pseudomonas monteilii" patented technology

The invention provides a Pseudomonas monteilii KY-05 with the collection number of CGMCC No.10172, which has cellulose degradation capacity. The Pseudomonas monteilii KY-05 has an enhancement effect on the biogas yield and anaerobic reaction start speed in the existing sludge anaerobic digestion technique, can be used for developing the microbial preparation for enhancing anaerobic digestion gas production efficiency, is gradually subjected to practical application and popularization in sludge plants, and has wide application prospects.

The invention relates to a novel microorganism namely pseudomonas monteilii CM strain producing hydantoinase and N-carboxamide hydrolase at the same time, a preparation method of the strain, and an application of the strain to synthesis of D(-)-4-hydroxyphenylglycine. The invention screens out a strain which can produce high-activity hydantoinase and N-carboxamide hydrolase. The hydantoinase and the N-carboxamide hydrolase prepared from the strain are used for catalyzing D, L-4-hydroxyphenyl hydantoin for synthesis of the D(-)-4-hydroxyphenylglycine, the conversion rate can reach 99% or above,and a new green high-efficiency convenient method for synthesizing the D(-)-4-hydroxyphenylglycine is provided.

The invention provides a Pseudomonas monteilii SCUEC2 strain, which has a preservation number of CCTCC NO:M2014134 and a nucleotide sequence shown as SEQ ID NO.1. The strain not only can be applied to nicotine degradation, but also can accelerate tobacco leaf ripening. The invention also provides a method for screening and separation of the strain. The method comprises the steps of: firstly conducting enrichment culture, then carrying out separation and purification, and finally inoculating the separated and purified strain into a nicotine medium by single colony, performing shake cultivation at room temperature for 1-3d, then measuring the content of nicotine in the fermentation broth of the nicotine medium, and re-screening the strain according to the nicotine degradation rate, thus obtaining the Pseudomonas monteilii SCUEC2 strain.

The invention belongs to the field of environmental treatment and particularly relates to application of a pseudomonas monteilii-containing microbial agent to purifying sulfurous gas. Specifically, the pseudomonas monteilii-containing microbial agent is Pseudomonas monteilii LW-1 with a preservation number of CGMCC No. 13748. The pseudomonas monteilii-containing microbial agent is applied to processing organic sulfur-containing waste gas produced from a pesticide wastewater pool; during application, the gas flow is 100-5000 m3 / h, the retention time is 1.0-5.0 min, the temperature is 35-55 DEGC, the total concentration of malathion, parathion and methyl-parathion is 20-200 mg / m3, and the degrading efficiency of the malathion, the parathion and the methyl-parathion is up to higher than 90%.

The invention discloses an indole-degrading compound bacterial agent and a reaction device thereof. The compound bacterial agent is prepared from three indole-degrading microbial strains including pseudomonas monteilii QM, bacillus L1 and Tolumonas G1. The reaction device comprises an aeration mixing reaction area, an aeration device, a bacterial agent feeder with the indole-degrading compound bacterial agent, an overflow area, an inclined-plate precipitation area, a residual sludge discharge pipe and a sludge backflow device. The indole-degrading compound bacterial agent disclosed by the invention has strong practicability; under the joint effect with the compound bacterial agent, the pollutant degrading efficiency of aerobic active sludge is high; the compound bacterial agent can adapt to the environment within short time so as to culture and acclimatize an efficient flora suitable for indole wastewater; the indole and COD detected in the water both can reach the standard; and the problems of limitation of existing methods in treating nitrogen heterocyclic ring organic matter indole and low treatment efficiency of existing bacterial agents are solved.

The invention belongs to the field of environmental treatment and particularly relates to a microbial agent containing pseudomonas monteilii and application thereof. The pseudomonas monteilii is specifically pseudomonas monteilii LW-1; the preservation number of the pseudomonas monteilii is CGMCC No.13748. The microbial agent is used for treating organic sulfur-containing waste gas generated by apesticide wastewater storage tank; the gas flow is 100-5,000m<3> / h; the residence time is 1.0-5.0 minutes; the temperature is 35-55 DEG C; the total concentration of malathion, parathion and parathion-methyl is 20-200mg / m<3> or the concentration of trichloracetic aldehyde is 5-100mg / m<3>; the degradation rate of malathion, parathion and parathion-methyl can reach 90% or above; the degradation rateof trichloracetic aldehyde can reach75% or above.

The invention discloses a preparation method of chiral benzyl sulfoxide, which comprises the following steps: marking Pseudomonas monteilii ZMU‑T05 with the preservation number: CCTCC NO: M2013683 on an LB medium plate sterilized at 121°C for 20 min After static culture at 28 °C for 12 h, a single colony was picked and streaked on an M9 medium plate as a seed, induced by toluene for static culture, and stored at 4 °C after 24 h of growth; conventional cell culture; conventional biotransformation. The product obtained by the invention has high optical purity, low cost, safety and environmental protection.

The application has screened and obtained a microorganism that can utilize oil. The microorganism is Pseudomonas monteilii (Pseudomonas monteilii) XZL-13, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 17514. The optimum temperature of the microorganisms is 28°C, the optimum pH value is 7.0, the optimum salinity is 2g / L, and the time to reach the maximum culture biomass is 7h. It has the corresponding ability to use 7 kinds of edible oils including corn oil, animal oil, peanut oil, sesame oil, coconut oil and olive oil.

The invention discloses high-activity composite solid particles prepared from autotrophic-heterotrophic denitrification flora and a preparation method. The composite solid particles comprise the following components in percentage by weight: 15%-20% of thiobacillus denitrificans, 30%-40% of bacillus subtilis, 15%-25% of pseudomonas mongolica and 25%-30% of alcaligenes faecalis. The autotrophic-heterotrophic synergistic denitrification effect of thiobacillus denitrificans, bacillus subtilis, pseudomonas monteilii and alcaligenes faecalis is utilized, the denitrification process can be completedmore completely, nitrogen organic pollutants and other components in the bottom mud can be effectively degraded, hydrogen sulfide and other odorous gases are eliminated, the transparency of the waterbody is improved, the effect of in-situ remediation of the black and odorous bottom mud is achieved, and the method has the advantages of long remediation effect duration and cost saving.

The invention belongs to the field of environmental treatment, and in particular relates to the application of a bacterial agent containing Pseudomonas monsonii in purifying chlorine-containing waste gas. The Pseudomonas monteilii is specifically Pseudomonas monteilii (Pseudomonas monteilii) LW-1, the preservation number is CGMCC No.13748, and the bacterial agent is used to treat the organic chlorine-containing waste gas produced by the pesticide wastewater storage tank, Gas flow rate is 100‑5000m 3 / h, residence time 1.0-5.0min, temperature 35-55°C, chloral concentration 5-100mg / m 3 , the degradation efficiency of chloral can reach more than 75%.

The invention provides a kind of Pseudomonas montelii and its application, the Pseudomonas montelii PM-41 preservation number is CGMCC No.18491; ‑41 was activated, fermented and cultured and applied to the roots of Panax notoginseng. The Pseudomonas montellae PM-41 provided by the present invention has good disease prevention and growth promotion effects, can antagonize the Panax notoginseng rust bacteria, reduce the occurrence of Panax notoginseng rust, and at the same time can degrade the autotoxic substances secreted by Panax notoginseng , especially for saponins Rg1 and Rb1, the concentration is greatly reduced after 192 hours of inoculation, and the degradation rate reaches more than 15%; The emergence rate is above 20%, which obviously relieves the obstacles to continuous cropping.

The invention discloses a biological control pseudomonas monteilii strain against tobacco mosaic virus (TMV). The code name of the strain is 4A1, the strain is collected in China General Microbiological Culture Collection Center (CGMCC) on September 7, 2011, and the collection number of the strain is CGMCC No. 5229. The strain can inhibit the infection of the TMV, and a biological control experiment shows that the strain can control the TMV. Simultaneously, the invention further provides a method for separating active substances from fermentation supernatant fluid obtained from the strain, and the active substances have a very high application value in the aspect of controlling the TMV.

The invention discloses a method for repairing a uranium polluted water body by utilizing facultative marine microorganisms. According to the method, bacteria are utilized for biological reduction effect for U(VI) by putting living Pseudomonas monteilii in the uranium polluted water body so as to reach the effect of removing the U(VI) in the water body. The removal rate of uranium in U(VI)-containing waste water with the concentration of 0-100 mg / L can reach more than 80% through putting one time; and the method disclosed by the invention has the advantages of simple processing steps, convenient material taking, low cost, environmental protection, high repair efficiency and the like.

A kind of Pseudomonas monteilii and its application belong to the technical field of agricultural fertilizers. The Pseudomonas monteilii PN1 screened by the present invention provides a new microbial resource, which has multiple biological functions, It can not only degrade the residual p-nitrophenol in the soil, but also improve the tolerance and stress resistance of corn seedlings to abiotic stresses such as pesticide residues, drought and saline-alkali, which is of great significance for ensuring the safe, green and efficient production of corn and other crops Important guiding significance and application value.

The invention belongs to the field of environmental governance, and particularly relates to application of fungicide comprising pseudomonas monteilii in purification of chlorine-containing waste gas.The pseudomonas monteilii is particularly Pseudomonas monteilii LW-1, the accession number is CGMCC No. 13748, the fungicide is used for treating organic-chlorine-containing waste gas generated in a pesticide waste water storage pond, gas flow is 100-5000 m<3> / h, residence time is 1.0-5.0 min, the temperature is 35-55 DEG C, the concentration of trichloracetic aldehyde is 5-100 mg / m<3>, and the degradation efficiency of the trichloracetic aldehyde can reach 75% or above.

The invention belongs to the field of environmental governance, and particularly relates to a preparation method of microbial active filler comprising pseudomonas monteilii. The pseudomonas monteiliiis particularly Pseudomonas monteilii LW-1, the accession number is CGMCC No. 13748, the microbial active filler is prepared by adsorbing pseudomonas monteilii bacterial suspension on the filler and filling a net-surface hollow ball with the filler, the filler is used for treating organic-sulfur-containing waste gas generated by the pesticide waste water storage pond, gas flow is 100-5000 m<3> / h,the residence time is 1.0-5.0 min, the temperature is 35-55 DEG C, the total concentrations of malathion, parathion and methyl-parathion are 20-200 mg / m<3>, or the concentration of trichloracetic aldehyde is 5-100 mg / m<3>, the degradation efficiency of the malathion, the parathion and the methyl-parathion can reach 90% or above, and the degradation efficiency of the trichloracetic aldehyde can reach 75% or above.

The invention belongs to the biology engineering technical field, and relates to a method for preparing indigo by conversing indole through phenol-degrading bacteria. The invention is characterized in that the phenol-degrading microbial strain capable of effectively catalyzing indole to synthesize indigo is employed, and is Pseudomonas monteilii QM, and the preservation number is CGMCC No.5054; when the biotransformation reaction is carried out, the cell dry weight concentration of the Pseudomonas monteilii QM bacterium in a reaction solution is 0.1-10g / L, the indole concentration is 25-200mg / L, under temperature of 20-40 DEG C, the shaking table rotating speed is controlled with 50-300r / min, and the oscillation reaction lasts for 1-8 hours. The condition of the biotransformation reaction is mild, the product concentration reaches as high as 42.5mg / L, the production period is short, the cost is low, the extraction step is simple, the production step is clean, and the method is suitable for industrial production and application.

The invention clones, expresses and discloses a monooxygenase PMO-6814 from pseudomonas monteilii as well as application thereof, and particularly relates to a genetically engineered bacterium which has the activity of a monooxygenase and is prepared by cloning the encoding gene of the monooxygenase PMO-6814 from a pseudomonas monteilii genome, constructing an expression vector of the encoding gene and guiding the expression vector into escherichia coli. The monooxygenase protein has the amino acid sequence as shown in SEQ ID No.1, and the corresponding encoding gene of the monooxygenase protein has the nucleotide sequence as shown in SEQ ID No.2. After being reconstructed and expressed, the monooxygenase protein is capable of effectively catalyzing a thioether substrate to generate sulfoxide, has relatively high conversion efficiency and stereoisomeride enantioselectivity, and can be used for the biosynthesis of chiral sulfoxide medicines.

The invention belongs to the field of environmental governance, and particularly relates to application of bioactive fillers containing Pseudomonas monnieri in purifying sulfur-containing waste gases.The Pseudomonas monnieri is specifically Pseudomonas monteilii LW-1 having a preservation number of CGMCC No. 13748; the microactive fillers are prepared by adsorbing a suspension of Pseudomonas mongolidus on fillers, and filling the fillers in a mesh hollow sphere; the fillers are used for treating organic sulfur-containing waste gases generated in a pesticide wastewater storage tank, wherein the gas flow rate is 100-5000 m<3> / h; the residence time 1.0-5.0 min; the temperature is 35-55 DEG C; the total concentration of malathion, parathion and methyl parathion is 20-200 mg / m<3>; the degradation efficiency of malathion, parathion and methyl parathion can reach 90% or higher.