Pseudomonas monteilii and application thereof

A technology of Pseudomonas monsonii and bacterial agents, applied in the field of microorganisms, can solve the problems of continuous cropping obstacles, yield and quality decline of Panax notoginseng

Active Publication Date: 2019-12-13
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a Pseudomonas monsonii and its application to solve the problem in the prior art that the continuous cropping obstacle of Panax notoginseng causes its yield and quality to decline

Method used

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  • Pseudomonas monteilii and application thereof
  • Pseudomonas monteilii and application thereof
  • Pseudomonas monteilii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this example, a kind of Pseudomonas monteilii PM-41 was isolated from healthy Sanqi rhizosphere soil by plate separation method, and its morphology, physiological and biochemical characteristics were identified. The specific results are as follows: Gram-negative bacteria, non-spore-shaped, short rod-shaped, white colonies, smooth and raised surfaces, and irregular edges. The bacteria utilize α-D-glucose, D-galactose, D-mannitol, troleandomycin, rifamycin SV, L-aspartic acid, L-glutamic acid, L-arginine, L-histamine, lincomycin, guanidine hydrochloride, tetradecyl sodium sulfate, D-gluconic acid, mucic acid, quinic acid, vancomycin, tetrazolium blue, tetrazolium violet, citric acid, L-malic acid, γ-aminobutyric acid and sodium bromate conformed to the morphology, physiological and biochemical characteristics of Pseudomonas monsonii.

[0027] Pseudomonas monteilii (Pseudomonas monteilii) PM-41 provided by the present embodiment has stronger inhibitory activity to the ...

Embodiment 2

[0032] This example relates to the detection of the inhibitory effect of Pseudomonas monteilii PM-41 on rust pathogens.

[0033] Cylindrocarpon destructans tested in this example were provided by the Plant Pathology Laboratory of Plant Protection College of Yunnan Agricultural University.

[0034] The specific method is as follows:

[0035]The inhibitory activity of Pseudomonas monsonii PM-41 on the mycelial growth of the tested rust pathogen was determined by plate confrontation culture method. On the ultra-clean workbench, the rust rot bacteria grown on the PDA medium will be made into a bacterium cake with a puncher with a diameter of 5mm, and then the bacterium cake will be transferred to the center of the PDA medium plate, and then the bacteria cake will be transferred to the center of the PDA medium plate, and then the bacteria cake will be used aseptically. Use a toothpick to inoculate the isolated and purified Pseudomonas monsonii at a distance of 2.5cm from the patho...

Embodiment 3

[0043] This embodiment detects the degradation ability of Pseudomonas monteilii PM-41 to saponin autotoxic substances, and the specific steps are as follows:

[0044] (1) Pick the purified single Pseudomonas monsonii PM-41 strain with an inoculation loop, inoculate it into 50 mL of NA liquid medium, place it on a shaker at 28°C and 150r / min and cultivate it overnight; then, take Add 100 μL of bacterial solution into 200 mL of liquid medium with crude notoginseng saponin as the sole carbon source (1 g / L of crude notoginseng saponin), and take the treatment without adding bacterial solution as a blank control, and place it on a shaker for shaking culture (28°C , 150r / min), each treatment was repeated 3 times, and samples were taken for measurement at 96h, 144h and 192h of culture.

[0045] Among them, the formula of NA medium is glucose 10g, peptone 5g, beef extract 3g, yeast extract 1g, agar powder 17g, water 1000mL.

[0046] (2) Take 100mL liquid (including the blank control)...

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Abstract

The present invention provides pseudomonas monteilii and an application thereof. A preservation number of the pseudomonas monteilii PM-41 is CGMCC No.18491. The application method is as follows: afterthe pseudomonas monteilii PM-41 is activated, fermented and cultured, the pseudomonas monteilii PM-41 is applied in notoginseng root. The provided pseudomonas monteilii PM-41 has good disease prevention and growth promotion functions, can antagonize notoginseng root cylindrocarpon destructans, reduces occurrence of notoginseng root rusted root disease, and at same time can degrade autotoxic substances secreted by the notoginseng root, especially saponin Rg1 and Rb1, concentration of the autotoxic substances is significantly reduced after 192 hours of inoculation, and besides, a degradation rate reaches 15% or more. The provided pseudomonas monteilii PM-41 can effectively improve an emergence rate of the notoginseng root in continuous cropping soil by 20% or more and obviously relieves continuous cropping obstacles.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a pseudomonas monsonii and its application. Background technique [0002] Sanqi (Panax notoginseng (Burk.) F.H.Chen) belongs to the Araliaceae Ginseng plant. It is a rare and precious Chinese medicinal material unique to my country. Yunnan is the main producing area and origin of Panax notoginseng. However, there are serious continuous cropping obstacles in the growth of Panax notoginseng, and the continuous cropping obstacles have seriously affected the yield and quality of Panax notoginseng due to large-scale single planting in successive years. The saponins compound secreted by the root system of Panax notoginseng is an important cause of continuous cropping obstacle of Panax notoginseng. At present, chemical pesticides and crop rotation are mainly used to alleviate continuous cropping obstacles in production. However, the extensive use of pesticides not only does not...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 杨敏王罗涛朱书生杨冬英刘屹湘黄惠川何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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