Microbial agent containing pseudomonas monteilii

A technology of Pseudomonas monsonii and bacterial agents, applied in the field of environmental governance, can solve the problems of low organic chlorine treatment efficiency, uneven loading, and packing collapse, etc., and achieve the effects of short adaptation period, shortened start-up period, and less loss

Inactive Publication Date: 2018-08-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In order to solve the problems in the prior art of volatile organic sulfur, low organic chlorine treatment efficiency, uneven loading of microorganisms on the surface of the mixed carrier, slow start-up, and

Method used

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  • Microbial agent containing pseudomonas monteilii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 A bacterial agent for purifying exhaust gas containing organic sulfur

[0045] An inoculum prepared by Pseudomonas monteilii LW-1, which can be used to purify organic sulfur-containing waste gas and solve the problem of volatile organic sulfur pollution generated in the process of pesticide wastewater treatment. The preparation method of inocula is as follows:

[0046] (1) Preparation method of fermentation broth

[0047] Pseudomonas monseri LW-1 was cultured on a slope and then connected to the liquid fermentation medium. The culture was amplified step by step at 10% inoculum. Fermentation conditions: 35℃, ventilation 10L / min, dissolved oxygen> Under the condition of 2.0mg / L, tank pressure 0.03-0.10Mpa, culture time 55h to obtain fermentation broth;

[0048] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5g, NaCl0.5g, MgSO 4 0.2g, CaCl 2 0.05g, FeSO 4 0.02g, 1000ml water, 20g agar, pH 6.2-7.0, sterilize at ...

Embodiment 2

[0056] Example 2 A bacterial agent for purifying exhaust gas containing organic sulfur

[0057] An inoculum prepared by Pseudomonas monteilii LW-1, which can be used to purify organic sulfur-containing waste gas and solve the problem of volatile organic sulfur pollution generated in the process of pesticide wastewater treatment. The preparation method of inocula is as follows:

[0058] (1) Preparation method of fermentation broth

[0059] Pseudomonas monseri LW-1 was cultured on a slope and then connected to the liquid fermentation medium. The culture was amplified step by step according to 25% of the inoculum. Fermentation conditions: 45℃, ventilation 100L / min, dissolved oxygen> Under the condition of 2.0mg / L, tank pressure 0.03-0.10Mpa, culture time 45h to obtain fermentation broth;

[0060] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5g, NaCl0.5g, MgSO 4 0.2g, CaCl 2 0.05g, FeSO 4 0.02g, 1000ml water, 20g agar, pH 6.2-...

Embodiment 3

[0067] Load the above liquid inocula on the ceramsite filler, the concentration of Pseudomonas monseri LW-1 bacteria in the filler is 9.0×10 8 CFU / g ( filler ) , Used to treat waste gas containing organic sulfur from the pesticide wastewater storage tank, with a gas flow of 5000m 3 / h, residence time 5.0min, temperature 55℃, total concentration of malathion, parathion and methyl parathion is 132.5mg / m 3 The degradation efficiency of malathion, parathion and methyl parathion can reach 91.4%. Example 3 A bacterial agent for purifying exhaust gas containing organic sulfur

[0068] An inoculum prepared by Pseudomonas monteilii LW-1, which can be used to purify organic sulfur-containing waste gas and solve the problem of volatile organic sulfur pollution generated in the process of pesticide wastewater treatment. The preparation method of inocula is as follows:

[0069] (1) Preparation method of fermentation broth

[0070] Pseudomonas monseri LW-1 was cultured on a slant surface and then...

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Abstract

The invention belongs to the field of environmental treatment and particularly relates to a microbial agent containing pseudomonas monteilii and application thereof. The pseudomonas monteilii is specifically pseudomonas monteilii LW-1; the preservation number of the pseudomonas monteilii is CGMCC No.13748. The microbial agent is used for treating organic sulfur-containing waste gas generated by apesticide wastewater storage tank; the gas flow is 100-5,000m<3>/h; the residence time is 1.0-5.0 minutes; the temperature is 35-55 DEG C; the total concentration of malathion, parathion and parathion-methyl is 20-200mg/m<3> or the concentration of trichloracetic aldehyde is 5-100mg/m<3>; the degradation rate of malathion, parathion and parathion-methyl can reach 90% or above; the degradation rateof trichloracetic aldehyde can reach75% or above.

Description

Technical field: [0001] The invention belongs to the field of environmental treatment, and specifically relates to a bacterial agent containing Pseudomonasmonteilii LW-1 and its application in purifying volatile organic sulfur / organic chlorine waste gas. Background technique: [0002] Wastewater from pesticide production contains a large amount of organosulfur pesticides and organochlorine pesticide intermediates, with complex components, high concentrations and strong toxicity. Part of the products of organosulfur pesticides and organochlorine pesticides or their intermediates, such as parathion, malathion, methyl parathion, chloroacetaldehyde, phosphorus trichloride, etc., are volatile and most organic Sulfur and organochlorine pesticides have a pungent odor. [0003] Parathion (C 10 H 14 NO 5 PS) is a colorless oily liquid with a water solubility of 24mg / L (25°C). Slightly soluble in petroleum ether and kerosene, miscible with most organic solvents, and smells like garlic. It...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/70B01D53/48C12R1/38
CPCB01D53/48B01D53/70B01D53/84C12N1/20Y02A50/20
Inventor 李琳薛松王彦杰杨凯雄刘俊新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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