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A strain of Pseudomonas montseri and its application

A technology of Pseudomonas monsonii and preservation number, which is applied in the field of environmental governance and can solve the problems of slow startup, uneven load, and low efficiency of organochlorine treatment

Active Publication Date: 2019-12-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In order to solve the problems in the prior art of volatile organic sulfur, low organic chlorine treatment efficiency, uneven loading of microorganisms on the surface of the mixed carrier, slow start-up, and collapse of fillers after long-term use, the present invention provides a strain of Pseudomonas monsonii (Pseudomonas monteilii), and apply the strain to remove waste gas containing organic sulfur and organic chlorine

Method used

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  • A strain of Pseudomonas montseri and its application

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Embodiment 1

[0073] Embodiment 1 A kind of bacterial agent for purifying waste gas containing organic sulfur

[0074] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0075] (1) Fermentation broth preparation method

[0076] Pseudomonas monsonii LW-1 was cultured on a slant and then inserted into the fermentation medium. The inoculum was expanded step by step according to 10% of the inoculum. At 35°C, the ventilation rate was 10L / min, the dissolved oxygen was >2.0mg / L, and the tank pressure was 0.03 Under the condition of -0.10Mpa, the culture time was 55h to obtain the fermentation broth;

[0077] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5...

Embodiment 2

[0085] Embodiment 2 A kind of bacterial agent for purifying waste gas containing organic sulfur

[0086] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0087] (1) Fermentation broth preparation method

[0088] Pseudomonas monsonii LW-1 was cultured on a slant and then inserted into the fermentation medium. The culture was scaled up step by step according to the inoculum size of 25%. Under the condition of 0.03-0.10Mpa, the culture time is 45h to obtain the fermentation broth;

[0089] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5g, NaCl0.5g, MgSO 4 0.2g, CaCl 2 0.05g, FeSO 4 0.02g, water 1000ml, agar 20g, pH value 6.2-7.0,...

Embodiment 3

[0097] Embodiment 3 A kind of bacterial agent for purifying waste gas containing organic sulfur

[0098] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0099] (1) Fermentation broth preparation method

[0100] Pseudomonas monsonii LW-1 was cultured on a seed slant and then inserted into the fermentation medium, and the inoculum was scaled up step by step according to 18%. Fermentation conditions: at 40°C, ventilation rate 50L / min, dissolved oxygen>2.0mg / L, under the tank pressure 0.03-0.10Mpa condition, culture time 50h obtains fermented liquid;

[0101] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5g, NaCl0.5g, MgSO 4 0.2g, C...

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Abstract

The invention belongs to the field of environmental management, and in particular relates to a strain of Pseudomonas monsonii and its application. The Pseudomonas monteilii is specifically Pseudomonas monteilii (Pseudomonas monteilii) LW-1, the preservation number is CGMCC No.13748, and the strain is used to treat the organic sulfur-containing waste gas produced by the pesticide wastewater storage tank, the gas The flow rate is 100‑5000m 3 / h, residence time 1.0-5.0min, temperature 35-55°C, total concentration of malathion, parathion, methyl parathion is 20-200mg / m 3 , or the concentration of chloral is 5‑100mg / m 3 , the degradation efficiency of malathion, parathion and methyl parathion can reach more than 90%, and the degradation efficiency of chloral can reach more than 75%.

Description

Technical field: [0001] The invention belongs to the field of environmental treatment, and in particular relates to a strain of Pseudomonas monteilii LW-1 and its application in purifying volatile organic sulfur / organic chlorine waste gas. Background technique: [0002] Wastewater from pesticide production contains a large amount of organosulfur pesticides and organochlorine pesticide intermediates, with complex components, high concentrations, and strong toxicity. Some organosulfur pesticides and organochlorine pesticide products or their intermediates, such as parathion, malathion, methyl parathion, chloral, phosphorus trichloride, etc., are volatile, and most of them are organic Sulfur and organochlorine pesticides have a pungent odor. [0003] Parathion (C 10 h 14 NO 5 PS) is a colorless oily liquid with a solubility in water of 24mg / L (25°C). Slightly soluble in petroleum ether and kerosene, miscible with most organic solvents, has garlic odor. Rapidly hydrolyzed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/48B01D53/70C12R1/38
CPCB01D53/48B01D53/70B01D53/84B01D2251/95B01D2257/2064B01D2257/306C12N1/205C12R2001/38Y02A50/20
Inventor 李琳王彦杰杨凯雄刘俊新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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