Composite solid particles prepared from autotrophic-heterotrophic denitrification flora and preparation method

A technology of autotrophic denitrification and heterotrophic denitrification, which is applied in the field of high-activity composite solid particles and its preparation, can solve the problems of insignificant elimination of hydrogen sulfide gas, short duration of repair effect, and incomplete denitrification, etc. Eliminates odorous gases such as hydrogen sulfide, improves water transparency, and lasts for a long time

Pending Publication Date: 2020-11-13
HOHAI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims at the problems in the prior art that the denitrification rate is low, the microbial preparation carrier easily causes secondary pollution of the water body, the hydrogen sulfide gas elimination effect is not obvious, the denitrification effect is incomplete, and the repair effect lasts for a short time. - Highly active composite solid particles prepared by heterotrophic denitrifying bacteria. The composite solid particles are synergistically effected by autotrophic and heterotrophic denitrifying bacteria, which can complete the denitrification process more completely, increase the denitrification rate, improve the transparency of the water body, and eliminate Odor gas, has the advantages of long-lasting repair effect and cost saving

Method used

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  • Composite solid particles prepared from autotrophic-heterotrophic denitrification flora and preparation method

Examples

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Effect test

Embodiment 1

[0026] A highly active composite solid particle prepared by autotrophic-heterotrophic denitrifying bacteria is made of autotrophic denitrifying bacteria and heterotrophic denitrifying bacteria, the autotrophic denitrifying bacteria is Thiobacillus denitrificans; heterotrophic denitrifying bacteria Denitrifying bacteria include Bacillus subtilis, Pseudomonas monsonii and Alcaligenes faecalis.

[0027]The weight percentages of Thiobacillus denitrificans, Bacillus subtilis, Pseudomonas montmorillonii and Alcaligenes faecalis in the composite solid particles described in this embodiment are respectively: 20% of Thiobacillus denitrificans, 40% of Bacillus subtilis, 40% of Bacillus subtilis, Pseudomonas 15% and Alcaligenes faecalis 25%.

[0028] A method for preparing composite solid particles, comprising the steps of:

[0029] Step 1: Use an inoculation loop to take two loops each of Thiobacillus denitrificans, Bacillus subtilis, Pseudomonas monsonii and Alcaligenes faecalis prese...

Embodiment 2

[0041] The difference between this example and Example 1 is that the culture temperature of each strain described in step 1 is 35°C in the solid medium, and the culture time is 2 days; It is obtained by mixing 15% Thiobacillus denitrificans, 35% Bacillus subtilis, 20% Pseudomonas montmorillonum, and 30% Alcaligenes faecalis; the rest of the steps are the same as in Example 1, and the mixture of autotrophic-heterotrophic denitrifying bacteria Group prepared highly active composite solid particles.

[0042] Put the bottom mud and overlying raw water from the polluted river into a 2L beaker, mix evenly and let it stand, add high-activity composite solid particles, the dosage is 5mg / L and treat for 35 days, test the treated water samples, bottom mud The degradation rate of medium-nitrogen organic pollutants reaches 98.5%, the odor of water samples is eliminated, the transparency of water bodies is improved, and the repair effect lasts for 3 months.

Embodiment 3

[0044] The difference between this example and Example 1 is that the culture temperature of each strain described in step 1 is 33°C in the solid medium, and the culture time is 4d; It is obtained by mixing 18% Thiobacillus denitrificans, 30% Bacillus subtilis, 25% Pseudomonas montmorillonum, and 27% Alcaligenes faecalis; the rest of the steps are the same as in Example 1, and the mixture of autotrophic-heterotrophic denitrifying bacteria Group prepared highly active composite solid particles.

[0045] Put the bottom mud and overlying raw water from the polluted river into a 2L beaker, mix evenly and let it stand, add high-activity composite solid particles, the dosage is 5mg / L and treat for 35 days, test the treated water samples, bottom mud The degradation rate of medium-nitrogen organic pollutants reaches 98.9%, the odor of water samples is eliminated, the transparency of water bodies is improved, and the repair effect lasts for 3 months.

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Abstract

The invention discloses high-activity composite solid particles prepared from autotrophic-heterotrophic denitrification flora and a preparation method. The composite solid particles comprise the following components in percentage by weight: 15%-20% of thiobacillus denitrificans, 30%-40% of bacillus subtilis, 15%-25% of pseudomonas mongolica and 25%-30% of alcaligenes faecalis. The autotrophic-heterotrophic synergistic denitrification effect of thiobacillus denitrificans, bacillus subtilis, pseudomonas monteilii and alcaligenes faecalis is utilized, the denitrification process can be completedmore completely, nitrogen organic pollutants and other components in the bottom mud can be effectively degraded, hydrogen sulfide and other odorous gases are eliminated, the transparency of the waterbody is improved, the effect of in-situ remediation of the black and odorous bottom mud is achieved, and the method has the advantages of long remediation effect duration and cost saving.

Description

technical field [0001] The invention belongs to the technical field of in-situ restoration of bottom mud, and particularly relates to a high-activity composite solid particle prepared by autotrophic-heterotrophic denitrifying bacteria group and a preparation method thereof. Background technique [0002] Water pollution is an important environmental problem that exists all over the world. In the treatment of river pollution, the treatment of polluted sediment has always been the main difficulty. Pollutants enter the water body through rainwater leaching, soil erosion, industrial wastewater discharge, etc., and gradually settle and accumulate in the bottom mud of the water body. The humus in the bottom mud will adsorb and chelate the black-causing substances in the surrounding environment, aggravating the black and odorous level of the bottom mud, and the organic matter in the black and smelly bottom mud will be transformed to produce odorous gases such as hydrogen sulfide. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C02F3/34C12R1/01C12R1/125C12R1/38C12R1/05
CPCC12N1/20C02F11/02C02F3/34C02F2103/007C02F2303/02
Inventor 王煜李一平程月施媛媛王亚宁潘泓哲李金华
Owner HOHAI UNIV
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