120 results about "Thiobacillus denitrificans" patented technology

The invention relates to a composite microbial preparation for treating a black and odorous river and a preparation method thereof. The composite microbial preparation is made from: Thiobacillus denitrificans powder 40-60 parts, composite Bacillus powder 10-40 parts and composite Saccharomyces cerevisiae powder 10-40 parts. Strains for degrading sulfides and organics in black and odorous sediment and suitable for growing in the black and odorous sediment are specifically screened, the combination of Thiobacillus denitrificans efficient in degrading hydrogen sulfide and removing nitrogen, Bacillus amyloliquefaciens capable of generating rich enzymes such as protease, amylase and cellulase, Saccharomyces cerevisiae, Candida utilis and facultative anaerobic Bacillus licheniformis enables the preparation to quickly improve the water quality of the black and odorous river and remove sediment blackness and odor.

The invention relates to a compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate and a preparation method thereof. The use amount of calcium nitrate is reduced, and the time for treating the black and odorous rivers is shortened. The compound microbial preparation is prepared from 40-80 parts of thiobacillus denitrificans and thiocapsa roseoppersicina mixed powder, 10-60 parts of composite bacillus subtilis powder and 10-60 parts of yeast and lactic acid bacterium mixed powder. By means of the preparation, sulfide in black and odorous bottom sludge, organic matter and strains suitable for growing in the black and odorous bottom sludge are screened and degraded in a targeted mode; by means of thiobacillus denitrificans with efficient hydrogen sulfide degrading and denitrification capacity, sulfur-oxidizing bacteria of thiocapsa roseoppersicina with efficient hydrogen sulfide and ammoniacal nitrogen degrading capacity, bacilli rich in protease, amylase, cellulose and other enzyme systems, yeast and lactic acid bacteria, a small amount of calcium nitrate and the compound microbial preparation are thrown into the black and odorous rivers so that water quality of the black and odorous rivers can be quickly improved, the black and odorous bottom sludge can be removed quickly, and the thickness and the organic matter content of the bottom sludge can be quickly reduced.

The invention discloses a method for synchronously removing nitrogen and phosphorus in sewage containing nitrogen and phosphorus, belonging to the field of sewage treatment. The method comprises the steps of (1) screening thiobacillus denitrificans: screening thiobacillus denitrificans from anaerobic activated sludge and taking as target strain, wherein thiobacillus denitrificans utilizes sulphur source in ferrous sulfide to conduct autotrophic denitrification; (2) preparing fillers of a reactor: directly adding ferrous sulfide with the particle size smaller than 25mm in a reaction container; (3) adding sewage: feeding sewage to be treated into the reaction container, wherein the sewage to be treated is the sewage containing nitrogen and phosphorus with the pH value of 5.0-9.0; (4) conducting the autotrophic denitrification process: conducting the mixed reaction for 2 to 6d at the temperature of 10 DEG C-40 DEG C under the anaerobic condition in the reaction container, reducing NO2<-> and NO3<-> in water to be N2 by thiobacillus denitrificans while simultaneously oxidizing ferrous sulfide by thiobacillus denitrificans, and simultaneously, precipitating phosphorus in water by utilizing iron ions (being oxidation products); and (5) separating solid and liquid. According to the method, the technology for removing nitrogen by utilizing thiobacillus denitrificans and the technology for removing phosphorus by using iron ions are fused naturally, the reaction cost is low, and the processing effect is good.

The invention provides a wastewater treatment process of anaerobic ammonia oxidation and sulfur-based autotrophic denitrification coupled denitrification and desulphuration. The process is characterized in that a mixed culture of anaerobic ammonium oxidation bacteria and thiobacillus denitrificans is taken as a main part; in the same reactor, the temperature is controlled between 25-35 DEG C, the pH value of wastewater is adjusted to 7.5-8.0, and the hydraulic retention time is 2.5-10 hours; the concentration of ammonia in the wastewater calculated according to the element N is 40-300mg / L<-1>, the concentration of nitrite in the wastewater calculated according to the element N is 50-390mg / L<-1> and the concentration of sulfide in the wastewater calculated according to the element S is 30-170mg / L<-1>; the concentration ratio of the ammonia to the nitrite calculated according to the element N is 1:1-1.32, and the concentration ratio of the nitrite calculated according to the element S to the ammonia calculated according to the element N is 1:1.54-1.74. The process is a novel wastewater treatment technology for synchronically performing denitrification and desulphuration economically efficiently by using treatment of wastes with processes of wastes against one another.

The invention relates to a process for purifying culturing water mass by utilizing microorganism, wherein microbiological functional bacteria of four various physiological categories, including nitrifying bacteria and nitrosation bacteria, denitrogenation sulfur bacillus in denitrifying bacteria, sulfur bacteria, and photosynthetic bacteria are thrown into the culturing water mass, the throwing amount is 0.05-0.20% of the culturing water mass, while the ratio by volume of various bacteria thrown is, nitrifying bacteria and nitrosation bacteria : denitrogenation sulfur bacillus in denitrifying bacteria : sulfur bacteria : photosynthetic bacteria = (1-4) : (1.6í½3) : (2í½3.6) : (1í½4). The invention can substantially reduce the contaminant in the culturing water mass. íí

The invention relates to a low carbon nitrogen ratio micro contaminated water nitrogen removal method. A sulphur / limestone / forest tree waste composite nitrogen removal filling material is layered and placed in a up flow reactor, and anaerobic sludge is inoculated into the filling material; HRT is set and started for 8 hours, and raw water is pumped, and a thiobacillus denitrificans medium is continuously let in for dynamic culture domestication of sludge, until stable operation; after ten days of operation, Na2S2O3.5H2O in the thiobacillus denitrificans medium is reduced to a half, and domestication is continued until a biomembrane grows mature again, and then Na2S2O3.5H2O in the thiobacillus denitrificans medium is reduced to zero, thereby microorganisms can utilize the sulphur in the filling material as sulfur source for growth and breeding; after ten days of system operation, a stable biomembrane is finally formed, and a nitrogen removal treatment of normal micro pollution water is carried out. A forest tree waste is added in the filling material according to the invention, and heterotrophic denitrifying bacterium are increased, and a mixed nutrition type nitrogen removal system is formed, and the invention has the advantages of short domestication time, good anti-interference capability, good nitrogen removal effect without secondary pollution.

The invention provides a method for treating sewage by using an anaerobic membrane bioreactor to remove sulphur and nitrogen. The method comprises the steps that the anaerobic reactor containing activated sludge of thiobacillus denitrificans is provided, the to-be-treated sewage containing nitrate and / or nitrite and sulfide is led into the anaerobic reactor to be in contact with the thiobacillus denitrificans, the thiobacillus denitrificans takes the nitrate as an electron acceptor to oxidize the sulfide in the wastewater into elemental sulfur and reduce the nitrate and / or nitrite into nitrogen to remove the nitrate and / or nitrite at the same time, the sewage is further in contact with a separation membrane in a membrane component after the nitrate and / or nitrite and sulfide are removed through the thiobacillus denitrificans, the separation membrane stops most of the thiobacillus denitrificans and other suspension particles, the stopped thiobacillus denitrificans and other suspension particles are kept in the reactor or returned to the reactor, and a filtered liquid is the treated water body. According to the method for treating sewage by using the anaerobic membrane bioreactor to remove sulphur and nitrogen, the process is simple, the energy consumption is low, and sulphur and nitrogen are removed efficiently.

The invention discloses a biological treatment system of sludge odor and a method for treating the sludge odor by utilizing the same, relating to the field of solid waste treatment. The biological treatment system of the sludge odor comprises an odor collector, an odor fan, an intake pipe, a biological deodorization tower, an outlet pipe, an active carbon adsorption device, a gas monitor and a discharge pipe. The method for treating the sludge odor by utilizing the biological treatment system comprises the following steps of: (1) injecting a bacterial solution enriched with thiobacillus denitrificans and nitrobacteria, acclimatizing for three weeks to finish starting; and (2) charging odor into the biological treatment system for treating through the odor fan. According to the biological treatment system of the sludge odor and the method for treating the sludge odor by utilizing the same, disclosed by the invention, main components of the sludge odor are removed by utilizing thiobacillus denitrificans and nitrobacteria, soft-hard bilayer fillers are adopted and active carbon is adopted for the following adsorption, thus the treatment effect is ensured; in addition, the biological treatment system of the sludge odor and the method for treating the sludge odor by utilizing the same, disclosed by the invention, have the advantages of low energy consumption and operation cost, and high safety and reliability, and is suitable for odor treatment of a large / middle sludge treatment project.

The invention discloses compound bactericide for treating sewage and a sewage treatment method thereof, and belongs to the technical field of sewage treatment. The compound bactericide comprises the following active ingredients from following strains: rhodopseudomonas, anaerobic ammonium oxidation bacteria, candida utilis, thiobacillus denitrificans, white-rot fungi, lactobacillus plantarum, bifidobacterium, lactobacillus acidophilus, green nonsulfur bacteria, phosphorus-accumulating bacteria, aspergillus oryzae, nitrosomas, streptomyces, bacillus subtilis and propionibacterium acidipropionici. The invention also provides the method using the compound bactericide to treat the sewage. The compound bactericide and the sewage treatment method have the advantages that the effect of treating sewage is better, the technology is simple, the implementing is easy, the operability is strong, the pollution to environment is avoided, the industrial requirements are met, and the application prospect is better.

The invention discloses a microbial agent for purifying sanitary sewage and a preparation method of the microbial agent. Active ingredients of the microbial agent is formed by mixing the following raw material bacteria including thiobacillus denitrificans, bacillus subtillis, bacillus licheniformis, bacillus polymyxa, lactic acid bacteria, nocardia amarae, denitrifying bacteria, rhodopseudomonas palustris, thiophilic red pseudomonas, rhodospirillum, saccharomycetes and propionibacterium. By means of the microbial agent, cellulose, saccharides, fat protein, pathogenic bacteria, viruses and parasitic ovum in sanitary sewage and pollutants, such as inorganic salt which is high in the contents of nitrogen, sulphur and phosphorus, the BOD and COD removal effect is guaranteed, meanwhile good bactericidal ability is achieved, high inhibiting ability is achieved especially for pathogenic bacteria, and the effect of treating sanitary sewage is remarkable.

The invention relates to a subsurface wetland system capable of improving nitrogen and phosphorus removal effects of tail water in sewage plant and avoiding bioclogging. The subsurface wetland system comprises a water distribution zone, a purifying zone and a water collection zone which are sequentially arranged, wherein the water distribution zone is connected with a water inlet pipe, the water collection zone is connected with a water outlet pipe and a sulfur / limestone mixed matrix, a gravel matrix and zeolite matrix are sequentially arranged inside the purifying zone according to the water flowing direction from front to rear. Compared with the prior art, the subsurface wetland is combined with sulfur autotrophic denitrification to remove nitrate in the influent water; since thiobacillus denitrificans slowly grows and the proliferation rate of thiobacillus denitrificans is far lower than that of heterotrophic bacteria, when efficient denitrification nitrogen removal is performed in the subsurface wetland system, the clogging of the subsurface wetland system due to the mass proliferation of microbes cannot be caused and meanwhile, limestone in the mixed matrix is adopted to achieve the removal of phosphates in the influent water.

The invention belongs to the field of microorganisms and discloses a biological agent for treating ammonia-nitrogen-containing wastewater. The biological agent comprises rhodococcus ruber, micrococcus luteus, enterococcus faecalis, acinetobacter baumannii, arthrobacter crystallopoietes, thiobacillus denitrificans and pseudomonas fluorescens. The biological agent comprises multiple strains, has the advantages of reasonable compatibility and strong synergic effect and can be used for effectively removing ammonia nitrogen, sulfide, aniline substances and industrial COD in wastewater.

The invention discloses a method for deeply treating sewage by using calcined pyrite as a filtering material. The method is characterized in that calcined pyrite is adopted as the filtering material. Anaerobic microbes such as denitrifying bacteria, thiobacillus denitrificans, iron-oxidizing bacteria depending on nitrate, ammonia-oxidizing bacteria, and the like are loaded on the surface of the filtering material. Second-stage treatment discharged water from a domestic sewage treatment factory, eutrophic ground surface water, or aquaculture water is continuously delivered to a filtering tank loaded with calcined pyrite for treatment, until phosphorous concentration of discharged water exceeds 0.1mg / L. With the method provided by the invention, a comprehensive effect of synchronous nitrogen removing, phosphorous removing, and organic pollutant concentration reducing is provided. The treatment efficiency is high.

The invention discloses a composite microbiological preparation for governing a black-odor river, a preparation method and application in governance of the black-odor river. The composite microbiological preparation comprises photosynthetic bacterium, nitrifying bacterium, thiobacillus denitrificans, bacillus subtilis and candida. The preparation method has the advantages that the composite microbiological preparation is prepared from a variety of strains, and all the strains are reasonable in proportion, capable of being symbiotic and free of antagonistic effect; the carrier of the composite microbiological preparation is formed by wheat bran, saw powder, activated carbon and brick powder, and is easy to acquire and low in cost; the composite microbiological preparation is extensive in application and high in removal efficiency on nutritive salt ingredients in the black-odor river, such as nitrogen and phosphorus; the composite microbiological preparation has an obvious eliminating effect on odor gas sulfuretted hydrogen in the black-odor river; the composite microbiological preparation is high in activity, rapid in reproduction, environmentally friendly and free of secondary pollution.

The invention relates to a membrane biological treatment device and method for cooperative treatment of methane and sulfur and nitrogen-containing wastewater. The device provided by the invention comprises a gas membrane separation container provided inside with a plurality of gas membrane modules, a circulation type oxidation container for holding a filling material adhered with methane-oxidizing bacteria and sulfate reducing bacteria, and a suspension, an upflow-type regeneration container for holding a supernatant solution containing Thiobacillus denitrificans, a solid-liquid separation container and a gas guiding container. Methane is oxidized and sulfate is reduced in the circulation type oxidation container; separation effect of gas membrane modules make unoxidized methane return to the circulation type oxidation container for continuous oxidization, and make the sulfate reducing product hydrogen sulfide enter into the upflow-type regeneration container and be oxidized by nitrate into sulfate, as a regeneration oxidant; and at the same time, nitrate is reduced into nitrogen, so as to realize cooperative effective treatment of methane exhaust gas and sulfur and nitrogen-containing wastewater in two biochemical reaction zones. The device provided by the invention has the advanategs of compact structure, low energy consumption and simple operation.

The invention belongs to the technical field of water environment treatment and relates to a rapid treatment material for treating and purifying wastewater and bottom sludge in the wastewater. An in-situ rapid treatment material for a black and odorous water body and the bottom sludge in cities and towns is prepared from the following components in parts by mass: 2 to 15 parts of thiobacillus denitrificans, 3 to 30 parts of EM bacteria, 0.5 to 15 parts of nitrifying bacteria, 1 to 15 parts of activated clay, 1 to 15 parts of zeolite, 1 to 20 parts of calcium nitrate, 0.5 to 5 parts of sodium carbonate, 5 to 40 parts of aluminum sulfate and 1 to 10 parts of polyacrylamide. According to the in-situ rapid treatment material for the black and odorous water body and the bottom sludge in citiesand towns, disclosed by the invention, by uniformly mixing a repairing material with the black and odorous water body and the bottom sludge in situ, nitrogen, phosphorus, sulfur, organic matters and the like in the water body and the bottom sludge can be effectively removed and the odor is eliminated, so that the effect of controlling endogenous pollution of a river channel is realized. The rapidtreatment material disclosed by the invention has the advantages of convenience in operation, small engineering quantity, time saving and cost saving, no secondary pollution and obvious effect; and the self-purification ability of the water body is greatly improved.

The invention provides a microbial preparation for water deodorization. The active ingredients of the microbial preparation are prepared from, by weight, 25-35 parts of thiobacillus denitrificans, 15-25 parts of bacillus pumilus, 15-25 parts of candida utilis, 10-20 parts of lactobacillus acidophilus and 10-20 parts of pseudomonas stutzeri. After the microbial preparation is prepared, black and odorous sewage can be cleaned through the steps of sewage pretreatment, sewage re-treatment and microbial oxidation. The microbial preparation has the advantages that activity is high, reproduction is quick, environment friendliness is achieved, pollution is avoided, cost is low, and strains are reasonable in compatibility, symbiotic, coordinated and not antagonistic. The water deodorization efficiency is high, and especially the good removal effect is achieved for hydrogen sulfide and ammonia gas.

The invention discloses a method for joint treatment of lead ion pollution by using carbonate-mineralization microbe-thiobacillus denitrificans. The method includes the steps that nutritional ingredients like fertilizer containing urea and ammonia thiosulfate are applied to soil or a water body; the prepared carbonate-mineralization microbe (0.5*10<8>-5.0*10<8> cfu / mL) is added into the soil or the water body polluted by heavy metal according to the proportion of the soil (0.5-5.0L / m<2>) to the water body being 1:10-1:100; after the carbonate-mineralization microbe is cultivated for 1-5d, the prepared thiobacillus denitrificans with the bacterium concentration being 0.5*10<8>-5.0*10<8> cfu / mL is added according to the volume ratio of the soil (0.5-5.0L / m<2>) to the water body being 1:10-1:100 to be evenly stirred and mixed, and cultivation lasts for 3-15d; and the removal rate of heavy metal Pb2 reaches 83%-99%. The method is low in cost, high in efficiency, easy to implement and broad in application prospect.

The invention belongs to the technical field of wastewater treatment and particularly relates to a microbial agent suitable for treating urban wastewater and a preparation method thereof. The microbial agent comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, saccharomycetes, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria, phosphorus-accumulating bacteria, Rhodospirillum, Thiobacillus denitrificans, Sphingobacteria, Alcaligenes faecalis, Bifidobacterium, brown rot fungi,Geotrichum candidum, Mucor, Aspergillus oryzae, and Penicillium diversum. The preparation method includes: mixing well Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria,phosphorus-accumulating bacteria and Bifidobacterium, adding the mixture into clean soil, farming earthworms with the soil for 12-14 days, and isolating the earthworms and the soil to obtain earthwormcast containing the mixed bacteria; mixing the earthworm cast containing the mixed bacteria and other bacteria, and immobilizing to obtain the microbial agent.

A high-efficiency autotrophic denitrification water quality regulation bacterium agent is characterized by comprising thiobacillus denitrificans, photosynthetic sulfur bacteria and inorganic salt including nitrate, phosphate and the like for promoting bacterial growth. The thiobacillus denitrificans and photosynthetic sulfur bacteria respectively account for 80%-95% and 5%-20%, and the total salinity of the inorganic salt is 1.5%. A microbial compound bacterium agent is wide in application, and can be applied to blockage of sulfide in ageing oil, microorganism corrosion in an oil field oil extraction water treatment system, smoke desulfuration in power plants, sediment deodorization and H2S removal in municipal sewage and slaughter wastewater, denitrification and phosphorus control of eutrophication natural lakes, degradation of nitrate and nitrite in aquaculture, and the like. The bacterium agent has unique desulfuration and denitrification physiological properties, can perform synchronous desulfuration and denitrification, requires no addition of organic matter, has mild reaction conditions and remarkable effects, and can be applied to multiple fields. In addition, reaction products are safe and innocuous.

The invention discloses a composite algae fungicide for improving water quality of an aquaculture pond. The composite algae fungicide is prepared from the following raw materials in parts by weight: 15-25 parts of chlorella pyrenoidosa solution, 30-50 parts of selenastrum capricornutum solution, 15-25 parts of scendesmus quadricauda solution, 5-15 parts of photosynthetic bacteria fermented liquid, 10-20 parts of sphaeailus nalans fermented liquid, 10-20 parts of thiobacillus denitrificans fermented liquid, 5-15 parts of bacillus natto fermented liquid, 5-15 parts of saccharomycetes fermented liquid and 1-2 parts of sodium humate. In addition, the invention further provides a preparation method of the composite algae fungicide for improving water quality of the aquaculture pond. After the composite algae fungicide disclosed by the invention is applied to a water body, organic pollutants in the water are transferred, transformed and degraded by utilizing metabolic activities of microbes, the pollutant concentration in the water is reduced, synergistic interaction can be realized, harmful substances such as ammonia nitrogen, nitrous acid, hydrogen sulfide and the like are decomposed, and the water body environment of the aquaculture pond is improved.

The invention discloses a compound microorganism combination microbial agent, a preparation method and application thereof, a sludge treatment method for a black-odor river. The compound microorganism combination microbial agent comprises the three kinds of microbial agent combinations of a microbial agent A combination, a microbial agent beta combination and a microbial agent gamma combination, and the microbial agent A combination comprises the microbial strains of bacillus coagulans, lactic acid bacteria, saccharomycetes, aspergillus sojae and thiobacillus denitrificans; the microbial agent beta combination comprises the microbial strains of lactic acid bacteria, bacillus subtilis, photosynthetic bacteria and saccharomycetes; and the microbial agent gamma combination comprises the microbial strains of lactic acid bacteria, bacillus subtilis, saccharomycetes, bacillus licheniformis and bacillus coagulans. The compound microorganism combination microbial agent can be used for treatment of bottom mud of the black-odor river, the treatment period is short, the thickness of the bottom mud can be effectively reduced, the content of harmful matters in the bottom mud is reduced, the situation that nitrogen and phosphorus of a black and odorous water body exceed a standard can be solved, and odorous gas hydrogen sulfide in the water body can be eliminated.

The invention discloses a preparation for restoring ammonia nitrogen-containing industrial sewage. The preparation is prepared from the following components: 100 parts of thiobacillus denitrificans, 80 parts of bacillus licheniformis, 60 parts of sphingomonas, 50 parts of phanerochaete chrysosporium, 40 parts of arthrobacter globiformis, 30 parts of alcaligenes faecalis, 10 parts of pseudomonas aeruginosa and 10 parts of bacillus cereus. The invention also discloses a preparation method of the preparation. The preparation disclosed by the invention is suitable for the treatment of high-ammonia nitrogen industrial sewage, is energy-saving and environment-friendly, and has better application prospects.

The invention provides a microbial preparation for culture water body improvement, which is prepared from the following raw materials in percentage by weight: 25-30 wt% of Bacillus subtilis, 25-30 wt% of Bacillus licheniformis and 15-20 wt% of Thiobacillus denitrificans. The invention also provides a method for preparing the microbial preparation, which comprises the following steps: sequentially inoculating the Bacillus subtilis, Bacillus licheniformis and Thiobacillus denitrificans seed culture solutions into a culture medium, fermenting to obtain a bacterial solution, and mixing the microbial solution with a carrier and nutrient substances to obtain the microbial preparation. The microbial preparation provided by the invention can obviously improve the quality of the culture water body and enhance the flesh texture of the cultured animals.

The invention discloses a domestic sewage purifying agent and a preparation method thereof, wherein the domestic sewage purifying agent is prepared from the following components in parts by weight: 40 parts of diatomite, 20-50 parts of silica powder, 15-35 parts of a gypsum powder, 1-9 parts of ferrous chloride, 2-6 parts of sodium carbonate, 2-8 parts of an aluminum oxide powder, 1-5 parts of thiobacillus denitrificans, 3-7 parts of bacillus licheniformis, 1-7 parts of sodium carboxymethylcellulose, 30-50 parts of polyvinyl alcohol, and 0.01-0.05 part of Tween 80. Compared with the prior art, the obtained domestic sewage purifying agent and the preparation method thereof have the advantages of simple process and low cost, not only have the effect of a sewage purifying agent of the prior art, but also have strong purification effect of domestic sewage, and can greatly save the amount.

The invention belongs to the field of wastewater treatment and specifically disclosesrocess for treating desulfurization wastewater of a power plant. The process comprises the steps of preparing a biological preparation and treating the wastewater, wherein thiobacillus denitrificans, bacillus cereus, baumanii, rhodococcus and pseudomonas fluorescens are used. The process disclosed by the invention is suitable for treating the desulfurization wastewater of the power plant and can be used for effectively removing sulfur-containing compounds and industrial COD.

The invention discloses a denitrification composite bacterial agent, a preparation method and an application thereof, wherein the denitrification composite bacterial agent comprises a composite microorganism and an adsorbent; the composite microorganisms consist of the following bacterial fluids in volume ratio: Bacillus megaterium 27-32%, thiobacillus denitrificans 18-23%, Nitrobacteria 18-23%, Nitrosomonas 18-23%, denitrifying bacteria 18-23%. A method for prepare a denitrifying composite bacterial agent comprise fermenting Bacillus megaterium, thiobacillus denitrificans, nitrifying bacteria, nitrosomonas and denitrify bacteria to obtain respective bacterial liquid, mixing and centrifuging according to volume ratio to obtain composite microorganisms, mixing with adsorbent, and freeze drying to obtain denitrifying composite bacterial agent. The denitrification compound bacterial agent prepared by the invention has the advantages of strong environmental adaptability, fast action aging,strong denitrification ability, low cost, good effect, environmental protection and pollution-free, simple preparation, and positive industrial practicability.

The invention belongs to the field of bio-engineering, and relates to a method for cultivating high density Thiobacillus denitrificans (ATCC25259). The method comprises: injecting a bacterial suspension and a nutrition solution to an air-lift bioreactor according to a ratio of 10-100:1; introducing air to replace the traditional mechanical stirring to carry out aeration, and controlling the dissolved oxygen concentration level, wherein the aeration rate is 800-1200 L / h; and maintaining the pH value of the nutrition solution in the air-lift bioreactor at 6-8, maintaining the temperature at 25-35 DEG C, and maintaining the conductivity at 20-60 mS / cm. The bacteria cultivating method has advantages of bacterial cultivating and desulfurization test integration, easy and safe operation, fast bacteria cultivating, sufficient and stable dissolved oxygen supply, rich and strongly-targeting bacteria, diversified acclimation manner and the like.

The invention discloses a compound biological synergist for petrochemical wastewater treatment and a preparation method thereof. The compound biological synergist comprises a complex flora and nutrients, wherein the complex flora is Rhodopseudomonas, Rhodospirillum rubrum, Lactobacillus acidophilus, Pediococcus, Bacillus subtilis, yeast, Acinetobacter, Lampropedia sp. and Thiobacillus denitrificans, and the nutrients are sodium humate, glucose, citric acid and glutamic acid coagulate. The invention relates to the technical field of water treatment. By using the compound biological synergist for petrochemical wastewater treatment and the preparation method thereof, the problems that petrochemical wastewater is susceptible to impact and microorganism poisoning is well solved, By adding high-efficiency bacteria and nutrients, the invention enhances the impact resistance and toxicity resistance of the biochemical system, improves the effluent quality, can improve the load for treating organic pollutants and the stability of microbial structure, improves the ability of the biochemical system to resist toxic and harmful substances, and provides reliable guarantee for further improving the effluent quality.

The invention discloses a sulfur-containing bamboo fiber magnetic suspension filler, and belongs to the field of sewage treatment. The sulfur-containing bamboo fiber magnetic suspension filler is characterized by comprising a high-density polyethylene base material, a bamboo fiber, pyritic powder and a magnetic material. Due to high hydrophilicity and adsorbability of the bamboo fiber, the film formation rate of thiobacillus denitrificans on the surface of the filler is increased; a weak magnetic field is formed by the magnetic material, so that the growth of the thiobacillus denitrificans ispromoted, and the autotrophic denitrification rate is increased. The sulfur-containing bamboo fiber magnetic suspension filler can deeply treat sewage, and can remove nitrogen and phosphorus at the same time, thereby solving the problem of high operation cost caused by heterotrophic denitrification of an external carbon source.