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Denitrification compound bacterial agent and its preparation method and application

A compound bacterial agent, the technology of Thiobacillus denitrifica, which is applied in the field of compound bacterial agent for nitrogen removal and its preparation, can solve problems such as pollution, and achieve the effects of convenient use, strong environmental adaptability, and strong pertinence

Inactive Publication Date: 2019-01-25
合肥市东方美捷分子材料技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to provide a nitrogen-removing composite bacterial agent with strong environmental adaptability and strong denitrification ability and its preparation method and application, in order to effectively solve the pollution problem caused by nitrogen-containing sewage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The nitrogen-removing compound bacterial agent of the present invention comprises the following components in weight ratio: compound microorganism:adsorbent=1:1. Composite microorganisms are formed by mixing bacterial solutions according to the volume ratio: 30% of Bacillus megaterium, 20% of Thiobacillus denitrification, 20% of Nitrobacter, 20% of Nitrosomonas, and 10% of Denitrifying Bacillus. The adsorbent is obtained by mixing peat soil and diatomaceous earth in a mass ratio of 3:7.

[0032] Each strain in the composite microorganism is activated separately, the medium is: yeast crude extract 10g, peptone 15g, glucose 5g, NH 4 Cl 5g, K 2 HPO 4 5g, 20g agar, add ddH 2 O to 1L, PH=7.0, sterilized at 121°C for 20min. The medium required for seed liquid culture and expanded culture is: yeast crude extract 10g, peptone 15g, glucose 5g, NH 4 Cl 5g, K 2 HPO 4 5g, add ddH 2 O to 1L, PH=7.0, sterilized at 121°C for 20min.

[0033] After each strain in the composit...

Embodiment 2

[0036] The nitrogen-removing compound bacterial agent of the present invention includes the following components by weight: composite microorganism:adsorbent=1:4. Composite microorganisms are formed by mixing bacterial solutions according to the volume ratio: 30% of Bacillus megaterium, 20% of Thiobacillus denitrification, 20% of Nitrobacter, 20% of Nitrosomonas, and 10% of Denitrifying Bacillus. The adsorbent is obtained by mixing peat soil and diatomaceous earth in a mass ratio of 2:8.

[0037] Each strain in the composite microorganism is activated separately, the medium is: yeast crude extract 10g, peptone 15g, glucose 5g, NH 4 Cl 5g, K 2 HPO 4 5g, 20g agar, add ddH 2 O to 1L, PH=7.0, sterilized at 121°C for 20min. The medium required for seed liquid culture and expanded culture is: yeast crude extract 10g, peptone 15g, glucose 5g, NH 4 Cl 5g, K 2 HPO 4 5g, add ddH 2 O to 1L, PH=7.0, sterilized at 121°C for 20min.

[0038] After each strain in the composite micr...

Embodiment 3

[0041] The nitrogen-removing compound bacterial agent of the present invention includes the following components by weight ratio: compound microorganism:adsorbent=1:2. Composite microorganisms are formed by mixing bacterial solutions according to the volume ratio: 30% of Bacillus megaterium, 20% of Thiobacillus denitrification, 20% of Nitrobacter, 20% of Nitrosomonas, and 10% of Denitrifying Bacillus. The adsorbent is obtained by mixing peat soil and diatomaceous earth in a mass ratio of 3:7.

[0042] Each strain in the composite microorganism is activated separately, the medium is: yeast crude extract 10g, peptone 15g, glucose 5g, NH 4 Cl 5g, K 2 HPO 4 5g, 20g agar, add ddH 2 O to 1L, PH=7.0, sterilized at 121°C for 20min. The medium required for seed liquid culture and expanded culture is: yeast crude extract 10g, peptone 15g, glucose 5g, NH 4 Cl 5g, K 2 HPO 4 5g, add ddH 2 O to 1L, PH=7.0, sterilized at 121°C for 20min.

[0043] After each strain in the composite...

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Abstract

The invention discloses a denitrification composite bacterial agent, a preparation method and an application thereof, wherein the denitrification composite bacterial agent comprises a composite microorganism and an adsorbent; the composite microorganisms consist of the following bacterial fluids in volume ratio: Bacillus megaterium 27-32%, thiobacillus denitrificans 18-23%, Nitrobacteria 18-23%, Nitrosomonas 18-23%, denitrifying bacteria 18-23%. A method for prepare a denitrifying composite bacterial agent comprise fermenting Bacillus megaterium, thiobacillus denitrificans, nitrifying bacteria, nitrosomonas and denitrify bacteria to obtain respective bacterial liquid, mixing and centrifuging according to volume ratio to obtain composite microorganisms, mixing with adsorbent, and freeze drying to obtain denitrifying composite bacterial agent. The denitrification compound bacterial agent prepared by the invention has the advantages of strong environmental adaptability, fast action aging,strong denitrification ability, low cost, good effect, environmental protection and pollution-free, simple preparation, and positive industrial practicability.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a nitrogen-removing composite bacterial agent and its preparation method and application. Background technique [0002] With the continuous improvement of the level of urbanization, the expansion of urban population, and the continuous development of industry and agriculture, the garbage and sewage produced by it are increasing day by day, causing environmental pollution and serious bad smell, especially frequent occurrence of water pollution accidents, seriously endangering people, animal health and life. The extensive use of various chemical synthetic products, including chemical fertilizers, pesticides and synthetic detergents, has led to an increase in nitrogen-containing nutrients in water bodies, resulting in eutrophication of water bodies and deterioration of water quality. The reported denitrification technologies for this nitrogenous sewage mainly i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/11C12R1/01C02F101/16C02F101/38
CPCC02F3/34C02F2101/16C02F2101/38C12N1/20
Inventor 朱守诚桑建伟黄家榜杨宏星褚维发孙庆红程鹏飞
Owner 合肥市东方美捷分子材料技术有限公司
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