Composite microbial preparation for culture water body improvement
A technology for microbial preparations and water body improvement, applied in the direction of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problems of bacterial drug resistance, excessive residual amount of biological drugs in aquaculture, etc., and achieve high live bacteria content and species The effect of less and simple preparation method
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Embodiment 1
[0052] Raw material composition:
[0053] Bacillus subtilis 25wt%
[0054] Bacillus licheniformis 30wt%
[0055] Thiobacillus denitrificans 15wt%
[0056] Carrier and Nutrients 30wt%
[0057] Preparation:
[0058] step 1:
[0059] Respectively inoculate Bacillus subtilis and Bacillus licheniformis in the first culture medium under aseptic conditions, and culture single strain for 48 hours at 30°C-35°C to obtain the seed culture solution of Bacillus subtilis and Bacillus licheniformis, and the live bacteria The number is greater than 5.0×10 9 cfu / ml. Among them, the first medium is composed of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, iron sulfate 30 -35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% (including vitamin H2-2.3wt%, vitamin B12-15wt% aqueous solution) and the rest of water, the pH value is 6.5-6.8 .
[0060] Inoculate Thiobacillus denitrificans in the seco...
Embodiment 2
[0071] Raw material composition:
[0072] Bacillus subtilis 30wt%
[0073] Bacillus licheniformis 20wt%
[0074] Thiobacillus denitrificans 20wt%
[0075] Carrier and Nutrients 30wt%
[0076] Preparation:
[0077] step 1:
[0078] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus licheniformis and the seed culture liquid of Thiobacillus denitrifica were provided.
[0079] Step 2,
[0080] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.
[0081] Step 3,
[0082] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.
[0083] Wherein, based on the total weight of the matrix (carrier and nut...
Embodiment 3
[0087] Raw material composition:
[0088] Bacillus subtilis 25wt%
[0089] Bacillus licheniformis 25wt%
[0090] Thiobacillus denitrificans 15wt%
[0091] Carrier and nutrients 35wt%
[0092] Preparation:
[0093] step 1:
[0094] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus licheniformis and the seed culture liquid of Thiobacillus denitrifica were provided.
[0095] Step 2,
[0096] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.
[0097] Step 3,
[0098]Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.
[0099] Wherein, based on the total weight of the matrix (carrier and nutr...
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