The invention discloses a preparation method of a
forage bacillus subtilis powder. The preparation method comprises the following steps: first, the
bacillus subtilis strain is inoculated on a slant culture medium to carry on slant culture, then the slant is flushed with a sterile physiological-
saline, and a seed
bacteria suspension is obtained; second, the seed-
bacteria suspension is inoculated in a seed tank with the
inoculation amount of 3% to 5%,by volume, to carry on
fermentation culture, and a seed
bacteria liquid is obtained; third, the seed bacteria liquid is inoculated into a
fermentation cylinder to carry on the
fermentation culture using a
pressure difference method, and a
fermentation broth is obtained; and fourth, bacteria
sludge is obtained through bacteria-liquid separation of the
fermentation broth, and the
forage bacillus subtilis powder is obtained through
spray drying of the bacteria
sludge. Through the exploring of culture medium formulas and fermentation conditions, the problems that the bacteria
powder prepared by conventional methods has low viable bacteria count and low
spore transformation rate are well solved, the production efficiency is increased, and the cost of enterprises is reduced. The
forage bacillus subtilis powder prepared through the method of the present invention has high viable bacteria count and high
spore transformation rate.