91 results about "Bryophyte" patented technology

A fast-growing plant planting, shaping, carbon sequestration and comprehensive utilizing method includes: (1) planting and / or culturing fast-growing woody, herbal, algae, lichen or moss plants in a chosen land area or water body area; (2) performing cutting, harvesting or gathering when the fast-growing woody, herbal, algae, lichen or moss plants grow to the height or dimension suitable for cutting, harvesting or gathering after a period of time; (3) drying the cut, harvested or gathered fast-growing woody, herbal, algae, lichen or moss plants; (4) processing and shaping the dried fast-growing woody, herbal, algae, lichen or moss plants; (5) transporting the dried and formed biomass to a storage place for storing; and (6) comprehensively utilizing the dried fast-growing woody, herbal, algae, lichen or moss plants.

Soil-free living roof and wall systems comprising a living layer of Bryophytes, Lichen, And Vascular Epiphytes (BLAVE) on the surface of a thin, light-weight, flexible, fire-resistant mat of mineral wool having a density in the range of about 6-12 lbs / cu ft and thickness in the range of about ⅜″ to about ¾″. Mats are provided in modular units on the order of 2′×4′; no irrigation or growth medium is used. The mats are adhered to a roof or wall with adhesive or mastic, or may be secured with fasteners. In the preferred embodiment, modular units include flexible mineral wool mats over a base sheet of non-woven plastic fibers, on the order of from 2-4 mm thick, and a wide-mesh plastic netting over the BLAVE layer, sewn through to the base layer. Methods of mat module manufacture, innoculant mix production, installation and cultivation / propagation of BLAVE are disclosed.

The invention discloses a method for making a moss wall. The method comprises the following steps: making a framework of a moss wall module, establishing a moss culture medium, performing rapid propagation on the medium layer by using moss chips to form a green moss clump, and integrally installing the cultured moss wall module to the external wall of a building. According to the method disclosed by the invention, the moss wall which can be made in a modular manner is cultured by utilizing mosses, and therefore, not only is the variety of green plants expanded but also the service lives of urban green walls are prolonged and the maintenance difficulty of the urban three-dimensional green walls is reduced.

A method for controlling the rock desert zone by use of bryophyte and dendrobe includes such steps as collecting wild bryophyte, stretching to decrease its thickness to 1-2 mm, dividing it into squares (1-100 sq.mm), reproducing in hothouse for 1-2 months, culturing the dendrobe seedlings in hothouse, stretching the reproduced bryophyte to decrease its thickness to 1-2 mm, dividing it into squares (1-100 sq.mm), transplanting the bryophyte on the surface of bare rock, and transplanting the dendrobe seedlings in said bryophyte layer.

The invention discloses an artificial cultivation method for wild paphiopedilum hirsutissimum. The method comprises the following steps of: (1) preparing a cultivation matrix: taking 3 to 6 volume parts of broken brick, 2 to 4 volume parts of crushed bark, 2 to 4 volume parts of humus soil and 1 to 3 volume parts of moss plant, spreading a layer of asbestos tile in a greenhouse, spreading the broken brick on the asbestos tile, sequentially scattering 30 to 50 percent of crushed bark and 20 to 40 percent of humus soil on the broken brick, mixing the rest crushed bark and the rest humus soil uniformly, scattering the mixture on the humus soil, spreading the moss plant on the mixture, and thus obtaining the cultivation matrix, wherein the thickness of the broken brick layer is 4 to 6 centimeters; and (2) planting the wild paphiopedilum hirsutissimum on the cultivation matrix, spraying a nutritional solution, and performing conventional management. By the method, the wild paphiopedilum hirsutissimum can be successfully transplanted in the greenhouse and artificially cultivated and domesticated, and the wild paphiopedilum hirsutissimum transplanted in the greenhouse can be normally grown and developed and normally flower in May to June; and a feasible method is provided for protection and sustainable development and utilization of the wild paphiopedilum hirsutissimum.

The invention discloses a cultivation method for planting dendrobium candidum seedlings in a forest. The cultivation method comprises the steps that (1) selecting trees and conducting pretreatment, (2) selecting the dendrobium candidum seedlings and preparing bryophyte, (3) binding dendrobium candidum seedlings to the sections of the trees, (4) cultivating the dendrobium candidum seedlings and (5) transplanting the dendrobium candidum seedlings. By the adoption of the cultivation method for planting the dendrobium candidum seedlings in the forest, the survival rate of the dendrobium candidum seedlings planted in the forest can reach over 90%, in this way, the cost of planting the dendrobium candidum seedlings in the forest is reduced, the time for the dendrobium candidum seedlings to be adapted to the environment in the field is shortened, and the technical support is provided for the purposes that dendrobium candidum medicine can be free of pollution and green, rapid growth is promoted, the yield is increased, and sustainable development is achieved.

The invention discloses stress-resistant protein PpLEA3-17 of bryophyte as well as an encoding gene and an application thereof. The protein related to the plant stress resistance and provided by the invention is named as PpLEA3-17, is from physcomitrella patens and is the protein in 1) or 2): (1) the protein consisting of amino acid sequences shown by the sequence 2 in a sequence table; and 2) the protein which is obtained by the amino acid residue sequence of the sequence 2 in the sequence table through substitution and / or deletion and / or addition of one or more amino acid residues, is related to the plant stress resistance and is derived by 1). The experiment for introducing the encoding gene of the PpLEA3-17 disclosed by the invention into wild type rice (Nipponbare) can prove that thesurvival rate (80.4%, 68.1%, 91.8% and 69.4%) and the growing state of four T2-substituted trans-PpLEA3-17-gene plant (17-1, 17-2, 17-3 and 17-4) in the drought stress experiment are obviously betterthan those of the control plant.

The invention discloses a bryophyte reversal-resistant protein PpLEA3-20 and an encoding gene and an application thereof. A plant resistance-related protein provided by the invention is named PpLEA3-20, is derived from physcomitrellapatens, and is a protein shown as (1) or (2), wherein (1) is constituted by an amino acid sequence shown as a sequence 2 in a sequence table, or (2) is obtained by performing substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid residue sequence shown as the sequence 2 in the sequence table, is relevant to plant resistance and is derived from (1). As proved by an experiment for introducing the encoding gene of PpLEA3-20 into oryza sativassp.japonica, the survival rates (44 percent, 36 percent, 60 percent and 72 percent) and growth states of four obtained T2-generation PpLEA3-20 transgenic plants (20-1, 20-2, 20-3 and 20-4) in a drought stress experiment are remarkably better than those of a check plant.

A Chinese herbal composition for environmental sanitation is free of toxin and user-friendly water-soluble spray. The practical applications of this spray include pest annihilation, better environmental sanitation, germicide and viruses inhibitive functions. It is also effective in prevention against epidemic and prevalent disease. This composition is composed of Akebia trifoliata, Polygonum perifoliatum, Euodia rutaecarpa, Stemona tuberose, Bryophyllum pinnatum, and Sophora flavescens. These drugs have passed the western and oriental pharmacologists' pharmacological experiments and proved of pesticide, germicide and viruses inhibitive effects. As based on above, the inventor also concerns the multiple effects of drug composition. By using revolutionary extraction and scientific preparation methods, the non-toxic Chinese herbal ingredients can be transformed into potent annihilative preparation.

The invention relates to the field of composite fertilizers and specifically relates to an active controlled-release composite fertilizer containing wine cellar mud. The fertilizer is prepared from the following materials in parts by weight: 10-15 parts of 1250-2000-mesh diatomite, 12-14 parts of feces of livestock and poultry, 8-10 parts of bryophyte, 6-8 parts of plant ashes, 2-3 parts of bamboo vinegar, 5-8 parts of waste coffee grounds, 3-5 parts of zinc carbonate, 10-12 parts of calcium hydrophosphate, 7-8 parts of saw dust, 10-12 parts of fish pond sludge, 2-4 parts of active carbon, 5-8 parts of tea stems, 8-10 parts of wine cellar mud, 2-4 parts of angled bittersweet stems, 4-6 parts of calcium superphosphate, 1-3 parts of lemon peel, 0.1-0.2 part of nano zinc sulfide, 6-8 parts of carboxymethyl chitosan, 1-3 parts of epoxy soybean oil, 4-5 parts of glycerol, 12-15 parts of polyvinyl alcohol, 1-2 parts of lauryl sodium sulfate and 4-5 parts of auxiliaries. The composite fertilizer disclosed by the invention is wide in formula source, contains various materials which are cheap, easily available, and rich in active biomass, can effectively improve soil environment, can promote crop growth, utilizes diatomite and a controlled-release film-coating agent to form dual coating for nutrients, is more lasting in fertilizer effect, and obviously improves crop yield and quality.

The invention discloses a rice composite fertilizer. The rice composite fertilizer comprises following raw materials: livestock and poultry feces, bryophyte, plant ash, alga extract and cake fertilizer. The alaga extract is prepared by following steps: alga is cut into pieces; water with a weight being 3 to 5 times that of alga is added; the pH value is adjusted to 8 to 9 with sodium hydroxide with a mass concentration of 40%; and the alga pieces are subjected to alkaline hydrolysis in boiling water for 30 to 40 min so as to obtain the alga extract. The rice composite fertilizer is composed of organic fertilizers such as livestock and poultry feces, bryophyte, plant ash and cake fertilizer, is green and friendly to the environment; the raw materials are cheap; the alga extract is rich in a plurality of active substances such as organic matter, carbohydrate, amino acid, inorganic salt, vitamin and phytohormone, is capable of protecting crops well, and reducing diseases.

The invention discloses a sweet osmanthus seedling substrate which is prepared from the following components in parts by weight: 15-42 parts of bryophyte, 15-33 parts of loess, 1-28 parts of mushroom residues, 18-42 parts of straws, 10-30 parts of peat, 8-25 parts of vermiculite, 1-3 parts of perlite, 1-5 parts of plant ashes, 0.2-2 parts of an EM bacteria solution, 0.5-0.8 part of activation powder and 1-3 parts of a water-retaining agent. The invention further discloses a preparation method of the sweet osmanthus seedling substrate. The substrate has a significant effect on promoting of growth of sweet osmanthus seedlings, has slight acidity of which the pH is smaller than 6.9, and is especially suitable for the acid-loving characteristic of the sweet osmanthus; the substrate contains a lot of organic matters, so that the substrate is balanced in nutrients; the substrate has the advantages of being sanitary, fertilizer-saving, few in diseases and pests and high in water retention capacity and has very high use value and economic value; and mass production is easy to implement.

The invention discloses a method for controlling turf degradation by using bryophytes and herb-bush plants combined with high-order granule technology, and belongs to the technical field of vegetationecological restoration. The method uses a combination of bryophyta gametophyte and herb-bush plants as a novel grass seed spraying agent, which conforms to a natural ecological succession process. The bryophytes can rapidly multiply under suitable conditions by utilizing the superiority of the bryophytes, and the germination rate and coverage of herbaceous plants are improved to form a more stable ecosystem so as to prevent current excessive turf degradation and large-scale open-air rock and soil and accelerate the formation of an organic whole of slope rock mass and vegetation matrix. A soilculture medium that is more suitable for plant growth is produced by using the high-order granule technology, and spray seeding is carried out by using professional equipment so as to provide betterprotection for the plant growth. Plant species are determined according to local climate, soil and water and site conditions, and the spray seeding is carried out according to the mass ratio of bush:herb: bryophytes=7:2:1. Artificially forced greening changes into self-reproduction of natural vegetation. The method of the invention is applicable to places where turf is seriously degraded or unableto grow, such as various slopes, barren mountains and acid-alkaline soil.

The invention relates to a humic acid incrusting dust suppressant for barren greening and a preparation method thereof. The humic acid incrusting dust suppressant for barren greening comprises the following components in parts by weight: 4-8 parts of humic acid, 2-6 parts of galactomannan gum or sodium carboxymethyl cellulose (CMC) and 1000 parts of water. The humic acid incrusting dust suppressant also comprises a preservative which accounts for 0.4-0.8% of the total weight except water, and moss seeds which account for 0.5-2% of the total weight of the humic acid incrusting dust suppressant. The humic acid incrusting dust suppressant can be used in various barrens in need of greening and dust suppression, can obviously reduce the flying dust pollution on various bare surfaces after being sprayed, and utilizes bryophytes to green the barren. The bryophytes and humic acid supplement each other; the humic acid provides necessary organic nutrients for the bryophytes; and the bryophytes can effectively increase the water retention property of soil on the premise of greening the bare surface, thereby performing the function of reducing the flying dust pollution on the ground surface.

The invention provides a method for producing an esthetic bryophyte bottle. The method comprises that the collected plants of funaria hygrometrica, entodon cladorrhizans, brachythecium albicans and the like are cut into small sections of about 2-5mm, and put into a transparent glass bottle which contains uniformly added mediums of perlite and the like and nutrient solution and is capped, and small holes are formed on a bottle cap for ventilation; culture is carried out for 50-110 days; and water and the nutrient solution need to be filled during the culturing and putting periods so as to main the bottle inside wet. By utilizing the method, the severe conditions of aseptic technique are overcome, the method is simple to operate, convenient and easy, low in cost, environmental-friendly and economic; the method is easy to master, has high plant survival rate, is convenient to popularize and has a wide market prospect; and when the produced esthetic bryophyte bottle which is artificially cultured is put on a desk, a user can enjoy gardens without going out.

The invention discloses an in-vitro rapid propagation method of macromitrium cavaleriei card and ther, which comprises the following steps: (1) carrying out surface sterilization of mature capsula of the macromitrium cavaleriei card and ther which grows in the natural environment; inoculating the mature capsula on Knop's culture medium without any hormone to culture and obtain protonemata; and then culturing for 30 days to obtain gametophyte; (2) inoculating single gametophyte on a culture medium for subculture multiplication of the gametophyte to obtain new gametophyte. The invention builds the in-vitro rapid propagation method of macromitrium cavaleriei card and ther for the first time; by using the in-vitro rapid propagation method, large-scale artificial propagation of the gametophyte of the macromitrium cavaleriei card and ther can be realized in a short time. The method has obvious scientific significance and practical value, and can provide a large number of uniformly-growing ideal test materials for indicating and monitoring atmospheric pollution by utilizing bryophytes. Simultaneously, the method continuously provides rich abundant seedlings for garden landscapes which use the bryophytes, such as establishing of a moss garden.

The invention relates to a rapid cloning method of plant flowering related gene LFY / FLO homologous fragments and special primers thereof, and relates to the technical field of rapid isolation and cloning of plant homologous genes. The method is characterized in that the used special primer LYF has a nucleotide sequence of 5'-TAYATIAAYAARCCIAARATG-3', and LYR has a nucleotide sequence of 5'-ARIYKIGTIGGIACRTACCA-3'; the primer concentration is 25 muM, and the annealing temperature is 44 DEG C. The method of the invention can clone to obtain homologous fragments of LFY / FLO genes of plants from bryophytes to spermatophytes, and has a wide range of the kinds of applicable plants; the potential problem that a gene fragment is difficult to be amplified due to no expression or low expression of homologous fragments of the LFY / FLO gene when cDNA is used as a template is well avoided; and the method is rapid, simple, economical, and effective.

The invention discloses a plant arrangement method based on diversity of ecological gardens. The method comprises the following steps: geography research, selection, breeding, layering, arrangement, maintaining and the like. Species are screened according to local reality to constitute communities, and perennial herbs, annual herbs, shrubs, arbors, vines and bryophytes are planted in different height layers. A garden landscape which is arranged by the arrangement method provided by the invention avoids species homogenization of a plurality of garden landscapes at present, has distinct gradations in vertical and horizontal directions, is close to the arrangement status in a natural state, has good ecological sustainability, is easy to keep and maintain, has low human participation, and has high promotional values.

The invention discloses a method for quickly growing a bryophyte crust on the cross section of an expressway slope. The method comprises the following steps: performing natural air drying on crust bryophytes together with very thin crust soil, and then smashing; uniformly spraying with water to wet fiber grid cells on an expressway slope, uniformly spreading the smashed soil-mixed bryophyte fragments on the cross section of a fiber grid, compacting the bryophytes with a flat tool, and covering with a plastic film; and pricking a certain number of pinholes in the film, and performing shading treatment on the film with a shading net to ensure that scattered light is received. According to the invention, the crust bryophytes are taken as basic materials, and the quick bryophyte propagation method is used for the cross section of an expressway, the cross section of mining area exploitation and other places where ecological environment of soil is destroyed, so that a bryophyte crust is formed on the surface of the soil, and the bryophyte crust can achieve the effects of rainfall interception and soil organic nutrient accumulation improvement, thereby laying a foundation for survival of advanced vascular plants in the later period.

A bryophyte tissue culture and seedling culture method based on direct spore induction comprises the steps of sporophyte selection and pretreatment, spore collection, spore direct disinfection, sporegermination induction, subculture mulitiplication of spores and protonemata, oscillatory differentiation culture, spray inoculation and seedling culture. According to the method, establishment efficiency and proliferation efficiency of a sterile system are improved, death rate and pollution rate are low, and effective seed feeding rate, subculture proliferation rate and seedling coverage rate after transplantation are high. Compared with the prior art, the tissue culture seeding death rate is reduced by 6.5%, the pollution rate is reduced by 70.4%, and the effective seeding rate is increased by 45.4%. The subculture proliferation rate is increased by 2.63 times, and the coverage rate of bryophyte plants cultured from seedlings reaches 85.6% on average.

The invention discloses a soil improving medium for saline-alkali land and a preparation method thereof. The soil improving medium is prepared by taking moss as an organic carrier to adsorb a mixed acid solution composed of 15-30% of humic acid, 5-15% of humic acid, 25-45% of cis-butenedioic acid, 2-10% of oxalic acid and 15-20% of sodium dioctyl sulfosuccinate, drying until the water content is less than 38% and then adding natural zeolite. As a moss plant in the soil improving medium has abundant sieve tubes and multiple gap microscopic structures, the soil improving medium is used as the carrier to be adsorbed in the sieve tubes of the moss plant and then the natural zeolite is added after the soil improving medium is dried. Therefore, the soil improving medium provided by the invention has the advantages of acidity, absorbability and solubility; the pH value of the soil is reduced, the salt content is reduced, the density is reduced and the organic matter is increased, therefore, the salt content in underground water is prevented from being raised.

The invention relates to an optimized formula for an improved matrix applied to ecological restoration of an acidic weathered coal gangue storage yard, belonging to the technical field of ecological restoration of mining wasteland. The invention aims to overcome the problems that conventional coal gangue storage yards are generally high in alien soil cost, severe in site condition and difficult for plants to survive and that common alkali neutralization methods and fertilization treatment are prone to failure. The optimized formula comprises, by volume, 10%-50% of limestone powder, 5%-50% of ground phosphate rock, 5%-30% of poultry manure organic fertilizer, 5%-30% of livestock manure organic fertilizer, 5%-30% of soil, 5%-10% of mushroom dregs, 5%-10% of grass meal and 1%-5% of bryophyte.The composite improved matrix is simple to prepare, wide in raw material sources and good in effect, replaces industrial wastes including residual sludge, fly ash and phosphogypsum with pollution risks in a repair matrix formula, and has good environmental benefits, economic benefits and social benefits.

The invention discloses a bionic double-protection seedling technology for taxus chinensis var mairei. The bionic double-protection seedling technology is mainly characterized in that sterilized seeds are sowed in a sterilized garden, and are covered; a shade shed is built immediately in the garden; and bryophyte plants are sowed on a seedbed in time to cover the seed to construct a bionic natural ecological environment to ensure that the seedlings can normally develop and grow so as to achieve the purpose of fast growing, high quality and high yield. The invention has the advantages of low cost for seedling culture, fast growing speed of seedlings, and high rate of emergence, and increases the yield by 30-40 percent compared with traditional open ground seedling culture; and over 80 percent of the seedlings can be qualified.

The invention discloses an in-vitro rapid propagation method of Brachymenium nepalense gametophytes, comprising the following steps of: (1) performing surface sterilization on capsulea of Brachymenium nepalense growing in a natural environment, and then, inoculating the capsulea to an improved Knop culture medium without any hormone to be cultured for 10 days, after spores germinate into protonemae, culturing 30 days again to obtain more protonemae after the growth of the primary protonemae; (2) transferring the protonemae obtained in (1) to a culture medium used for inducing the generation of the gametophytes, culturing for 30 days to obtain gametophyte branches; and (3) inoculating individual gametophyte branches obtained by induction culture in (2) to a culture medium for subculture multiplication of the gametophytes, and culturing for 30 days, wherein each gametophyte can be used to breed numerous new gametophytes. According to the in-vitro rapid propagation method of the Brachymenium nepalense gametophytes, the artificial propagation expanding of a large amount of Brachymenium nepalense gametophytes can be realized within a short time, a large amount of ideal materials are provided for indicating and monitoring atmospheric pollution by using bryophytes, and rich seedlings are provided for application of the bryophytes to garden landscaping.

The invention discloses an application of bryophyte as a disinfectant for plant explants, a disinfectant composition as well as a preparation method and a use method thereof. The composition comprises a bryophyte extract as an active component, a penetrant, a stabilizer and a latent solvent. The invention uses bryophyte to disinfect plant explants for the first time and greatly reduces the pollution rate of tissue culture. The raw materials of the disinfectant composition are from natural plants which are safe, non-toxic and harmless to the environment and plant tissues. The composition has simple preparation method, easy obtainment of raw material, low price and easy popularization and application.

The invention discloses a pesticide composition and application thereof in control of bryophyte. The pesticide composition includes an active ingredient A and an active ingredient B; wherein the active ingredient A is isothiazolinone, the active ingredient B is an ethylenebis compound, and the active ingredient A and the active ingredient B are in a weight ratio of 1:(1-11). The pesticide composition has the advantages of obvious synergistic effect in control of bryophyte, good and quick effect, sustained release effect, good persistency, less dosage, and no phytotoxicity, therefore reduces the environmental impact, and has lawn care benefits.

The invention discloses a high-survival-rate transplantation method for moss associated dendrobium officinale on a limestone surface. The method comprises the following steps of: on a base of which the exposure rate of limestone is high and the overshadowing rate is 50-70%, constructing a sunshade net, and laying an irrigation system, wherein the base is under an arboreal forest; cleaning the limestone surface of the base, ensuring that no humus soil is left on the limestone surface, laying the surface of the base with moss plants, and keeping moist and clean, wherein the thickness of the mossplants is 3-4 cm; after the moss plants survive, transplanting dendrobium officinale seeds and seedlings, and pressing the roots of the dendrobium officinale seeds to the surface of the moss plants;and carrying out sun shading by the sunshade net, adopting the irrigation system for keeping a base environment and the moss plants moist, and spraying a foliage fertilizer in a vigorous growth seasonto accelerate plants to grow and develop.

The invention discloses an ecological net for restoring vegetation on steep cliffs and a preparation method thereof, and belongs to the field of ecological restoration. The ecological net is a rope net formed by kinking a long rope made by wrapping moss pulverized pulp, microbial fertilizer, a light matrix and the like in a geotextile. Meanwhile, soil troughs are built at the foot of a mountain toplant vines and shrubs. The growth of the vines can accelerate the coverage of bare hillsides. One end of the ecological is connected to a water supply pipeline located at the mountain top for acquiring water from the water supply pipeline and transmitting the water along the extension direction of the ecological. The ecological net utilizes the characteristics of bryophytes to allow the bryophytes to grow on bare rock under the condition of guaranteeing a small amount of water and nutrient supply. The growth of the vines plays a certain role in stabilization, increases the biodiversity, andhas a certain landscape effect. The addition of microorganisms can continuously exert the fertilizer effect, promote the growth of plant roots, and achieve the purpose of ecological restoration. The ecological net is especially suitable for vegetation restoration on bare and soilless steep cliffs.

The invention discloses a preparation method of a moss-proof baking-free brick. The preparation method comprises the following operation steps: (1) uniformly mixing stainless steel slag, alunite and sal ammoniac, adding the mixture into a crusher for crushing, adding treating fluid into the mixture, and uniformly mixing and stirring the mixture to obtain a base material for later use; (2) uniformly mixing the base material, cement and fly ash, adding the mixture into a cement mortar mixer, carrying out stirring, adding water, a water reducing agent and an early strength agent into the mixer, continuing stirring the materials to obtain a mixture, adding the mixture into a mold, and carrying out compression molding to obtain bricks; and (3) taking out the bricks, and curing the bricks at room temperature for 15-20 days to obtain baking-free bricks. The moss-proof baking-free brick provided by the invention has excellent mechanical properties and high strength, is not easy to break duringcarrying and transportation, especially has excellent moss-proof properties, and can effectively prevent mosses and other bryophytes from growing and propagating on the surface of the brick.