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Bryophyte tissue culture and seedling culture method based on direct spore induction

A culture method and spore technology, applied in the fields of spore and protocelium induced proliferation, field seedling cultivation, explant selection and disinfection, can solve the problem of destroying the natural and ecological environment, large impact of environment and seasons, low spore germination rate, etc. problems, to achieve the effect of low spore mortality rate, pollution rate reduction and multiplication efficiency improvement

Active Publication Date: 2018-12-28
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of field excavations will seriously damage the natural and ecological environment. Artificial propagation is not only greatly affected by the environment and seasons, but also the propagation efficiency of cutting and branching is low, and the spore sowing and propagation has problems such as difficulty in collecting spores and low spore germination rate.

Method used

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  • Bryophyte tissue culture and seedling culture method based on direct spore induction
  • Bryophyte tissue culture and seedling culture method based on direct spore induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 (the inventive method)

[0037] (1) Selection and pretreatment of sporophytes

[0038] In October, when the spore capsules and spore peduncles of 'Moss calabash' turned yellowish brown, and the capsule caps had not yet fallen off, select well-developed sporophytes with full spore capsules, cut the spore capsules and put them in a petri dish, and first put them under 20W ultraviolet light. Cultivate under the lamp for 30 minutes, and then cultivate for 3 days under the sunlight with the light intensity of 3000 lx, and the cultivation temperature is 26°C.

[0039] (2) Spore collection

[0040] When the capsule cap of the spore capsule cultivated in step (1) begins to fall off, and when spores are shed, cut the capsule with a scalpel or gently pick the capsule cap with tweezers to allow the spores to disperse, and use the tweezers to remove the capsule stem and sporangia. After picking out other sundries, collect moss spores that can be used for tissue cultu...

Embodiment 2

[0061] Embodiment 2 (the inventive method)

[0062] Embodiment 2 is the same as Embodiment 1 except that the following steps are different.

[0063] (1) Selection and pretreatment of sporophytes

[0064] In August, when the spore capsules and spore peduncles of 'Physcomitrella angustifolia' turn yellowish brown and the capsule cap has not yet fallen off, use scissors to cut off the well-developed and full spore capsules and put them in a petri dish, and cultivate them under a 20W ultraviolet lamp first. 30 minutes, and then irradiate for 4 days at a temperature of 22° C. and a daylight intensity of 2500 lx.

[0065] (4) Induction of spore germination

[0066] Fill each culture bottle with 33ml of moss spore germination induction medium. Draw the mixed solution of the sterilized spores obtained in step (3) and hydrogen peroxide with a sterilized pipette or dropper to inoculate (drop on) the surface of the moss spore germination induction medium in the culture bottle, and sha...

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Abstract

A bryophyte tissue culture and seedling culture method based on direct spore induction comprises the steps of sporophyte selection and pretreatment, spore collection, spore direct disinfection, sporegermination induction, subculture mulitiplication of spores and protonemata, oscillatory differentiation culture, spray inoculation and seedling culture. According to the method, establishment efficiency and proliferation efficiency of a sterile system are improved, death rate and pollution rate are low, and effective seed feeding rate, subculture proliferation rate and seedling coverage rate after transplantation are high. Compared with the prior art, the tissue culture seeding death rate is reduced by 6.5%, the pollution rate is reduced by 70.4%, and the effective seeding rate is increased by 45.4%. The subculture proliferation rate is increased by 2.63 times, and the coverage rate of bryophyte plants cultured from seedlings reaches 85.6% on average.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and rapid propagation, and in particular relates to the technology of explant selection and disinfection, spore and protonema induced proliferation and field seedling cultivation in bryophyte tissue culture and rapid propagation. Background technique [0002] Moss (bryophytes) is a plant of the phylum Moss (Musci), and it is also a higher plant with the most widespread distribution, the largest variety, and the simplest structure in the world. Moss fresh plants have special habits such as growing in low light and high humidity environments, rich in water content, strong vitality, and thin spines. They play an important role in water and soil conservation, water conservation, nutrient circulation and storage in nature. With the development of urban and garden economy, the application and demand in garden landscaping, landscaping, moss turf, vertical greening, roof garden and ecological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/15A01G24/23
CPCA01G24/15A01G24/23A01G24/28A01G31/00A01H4/001A01H4/005
Inventor 吴丽芳杨春梅余蓉培屈云慧阮继伟单芹丽蒋海玉汪国鲜李帆
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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