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Composite microbial preparation for treating black and odorous river and preparation method thereof

A composite microbial agent and composite microorganism technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of accelerating the biodegradation of PAHs, nitrogen secondary pollution, and large oxidants

Active Publication Date: 2016-03-02
SHENZHEN DAZHI SHENGGUANG ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of river black and odor control, the current international popular method is to increase the oxidation-reduction potential of the sediment (such as adding calcium nitrate, calcium peroxide) to oxidize the sulfide in the sediment and inhibit the release of phosphorus in the sediment; among them, through Dosing calcium nitrate to eliminate river black odor has been applied in domestic and foreign projects. However, this technology has a long treatment cycle (1-2 years), and it is easy to promote the excessive release of ammonia nitrogen and nitrate, causing secondary pollution of nitrogen, etc. shortcoming
At present, some new attempts have been made at home and abroad to quickly eliminate the black and odor of sediment, such as adding other oxidants (calcium peroxide or hydrogen peroxide) while adding calcium nitrate, or shortening the treatment time by increasing the dosage of calcium nitrate. However, it is reported that the treatment cycle for the sulfide removal rate to reach more than 96% still takes about one month; Yu Guangwei and others disclosed an in-situ oxidation technology for black and odorous river sediments based on denitrification and desulfurization in Patent No. 201310230547.6. This technology includes adding sediment oxidizer (a mixture of calcium nitrate and sodium nitrate) by wet or dry method to black and odorous rivers, ammonia nitrogen extraction agent (sodium chloride or potassium chloride) and acid-base regulator (lime or dilute nitric acid), which can remove sulfur, nitrogen and phosphorus at the same time, quickly eliminate the black odor of the river, inhibit the release of nitrogen and phosphorus in the sediment, and improve the water quality. The main reason is to add some chemical oxidants to promote the denitrification and sulfur oxidation in the sediment Bacterial growth is used to degrade sulfide, but MurphyTP et al. believe that the addition of calcium nitrate can accelerate the biodegradation of PAHs (polycyclic aromatic hydrocarbons), but after adding calcium nitrate, the degradation of organic matter in different regions varies greatly
Phosphorus-containing organic matter can provide nutritional phosphate to overcome the lack of biologically available nutrients for nitrate-precipitated phosphate, but organic matter with low phosphorus content cannot raise enough phosphate
Calcium nitrate has a large difference in the inhibition of phosphorus in different regions, and therefore, the degradation of organic matter also shows a large difference
In addition, the biological oxygen demand of river polluted sediment is relatively high, and the oxidant that needs to be added in the restoration is relatively large, so there are problems of high cost and difficult implementation

Method used

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  • Composite microbial preparation for treating black and odorous river and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0061] A compound microbial preparation for controlling black and odorous rivers is mainly prepared by mixing the following raw materials in parts by weight: 50 parts of Thiobacillus denitrificans bacteria powder, 30 parts of compound Bacillus bacteria powder, and 20 parts of compound yeast bacteria powder.

[0062] A preparation method for a compound microbial preparation for controlling black and odorous rivers, comprising the following steps:

[0063] step : Preparation of Thiobacillus denitrification bacteria powder

[0064] A. Activation culture of Thiobacillus denitrificans semi-solid seeds: puncture the species of Thiobacillus denitrificans in semi-solid Thiobacillus denitrificans culture medium, culture at 30°C for 6 days, the bacterial line to be punctured grows a lawn, which can be used as activation Thiobacillus denitrificans

[0065] B. Cultivation of Thiobacillus denitrificans seeds: inoculate the activated Thiobacillus denitrificans species into the liquid cul...

Embodiment 2

[0092] A compound microbial preparation for controlling black and odorous rivers is mainly prepared by mixing the following raw materials in parts by weight: 55 parts of Thiobacillus denitrificans bacteria powder, 30 parts of compound Bacillus bacteria powder, and 15 parts of compound yeast bacteria powder.

[0093] The preparation method of the composite microbial preparation for controlling black and odorous rivers comprises the following steps:

[0094] step : Preparation of Thiobacillus denitrification bacteria powder

[0095] A. Activation culture of Thiobacillus denitrificans semi-solid seeds: puncture the species of Thiobacillus denitrificans in the medium of semi-solid Thiobacillus denitrificans, culture at 30°C for 7 days. Thiobacillus denitrificans

[0096]B. Cultivation of Thiobacillus denitrificans seeds: inoculate the activated Thiobacillus denitrificans species into the liquid culture medium of Thiobacillus denitrificans seeds, at a temperature of 30°C, anaero...

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Abstract

The invention relates to a composite microbial preparation for treating a black and odorous river and a preparation method thereof. The composite microbial preparation is made from: Thiobacillus denitrificans powder 40-60 parts, composite Bacillus powder 10-40 parts and composite Saccharomyces cerevisiae powder 10-40 parts. Strains for degrading sulfides and organics in black and odorous sediment and suitable for growing in the black and odorous sediment are specifically screened, the combination of Thiobacillus denitrificans efficient in degrading hydrogen sulfide and removing nitrogen, Bacillus amyloliquefaciens capable of generating rich enzymes such as protease, amylase and cellulase, Saccharomyces cerevisiae, Candida utilis and facultative anaerobic Bacillus licheniformis enables the preparation to quickly improve the water quality of the black and odorous river and remove sediment blackness and odor.

Description

technical field [0001] The invention relates to the technical field of treatment of black and odorous rivers, in particular to a composite microbial bacterial agent for rapidly controlling black and odorous rivers and reducing sediment and a preparation method thereof. Background technique [0002] With the development of my country's economy and the increase of population, the water consumption is constantly increasing, and a large amount of farmland runoff without effective treatment, industrial wastewater and domestic sewage are discharged into rivers, and some rivers are almost reduced to pollution-inducing rivers. The pollution concentration has far exceeded the self-purification capacity of the river, and most of the river has been in a state of black and odor for a long time, seriously affecting the life of residents along the river and the image of the city. A large number of water pollutants are silted up, the thickness of the sediment is increasing, and the cross s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C02F3/34C12R1/07C12R1/10C12R1/865C12R1/72
Inventor 刘津粼刘明英
Owner SHENZHEN DAZHI SHENGGUANG ENVIRONMENTAL PROTECTION TECH CO LTD
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