Composite microbial preparation for treating black and odorous river and preparation method thereof
A composite microbial agent and composite microorganism technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of accelerating the biodegradation of PAHs, nitrogen secondary pollution, and large oxidants
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Embodiment 1
[0061] A compound microbial preparation for controlling black and odorous rivers is mainly prepared by mixing the following raw materials in parts by weight: 50 parts of Thiobacillus denitrificans bacteria powder, 30 parts of compound Bacillus bacteria powder, and 20 parts of compound yeast bacteria powder.
[0062] A preparation method for a compound microbial preparation for controlling black and odorous rivers, comprising the following steps:
[0063] step : Preparation of Thiobacillus denitrification bacteria powder
[0064] A. Activation culture of Thiobacillus denitrificans semi-solid seeds: puncture the species of Thiobacillus denitrificans in semi-solid Thiobacillus denitrificans culture medium, culture at 30°C for 6 days, the bacterial line to be punctured grows a lawn, which can be used as activation Thiobacillus denitrificans
[0065] B. Cultivation of Thiobacillus denitrificans seeds: inoculate the activated Thiobacillus denitrificans species into the liquid cul...
Embodiment 2
[0092] A compound microbial preparation for controlling black and odorous rivers is mainly prepared by mixing the following raw materials in parts by weight: 55 parts of Thiobacillus denitrificans bacteria powder, 30 parts of compound Bacillus bacteria powder, and 15 parts of compound yeast bacteria powder.
[0093] The preparation method of the composite microbial preparation for controlling black and odorous rivers comprises the following steps:
[0094] step : Preparation of Thiobacillus denitrification bacteria powder
[0095] A. Activation culture of Thiobacillus denitrificans semi-solid seeds: puncture the species of Thiobacillus denitrificans in the medium of semi-solid Thiobacillus denitrificans, culture at 30°C for 7 days. Thiobacillus denitrificans
[0096]B. Cultivation of Thiobacillus denitrificans seeds: inoculate the activated Thiobacillus denitrificans species into the liquid culture medium of Thiobacillus denitrificans seeds, at a temperature of 30°C, anaero...
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