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Method for joint treatment of lead ion pollution by using carbonate-mineralization microbe-thiobacillus denitrificans

A technology of Thiobacillus denitrification and combined treatment is applied in the fields of resources and environment to achieve the effects of outstanding environmental friendliness, reduced treatment costs, and improved removal efficiency

Active Publication Date: 2017-01-04
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the fact that there is no relevant report on the application of the carbonate mineralizing bacteria-Thiobacillus denitrification system in the treatment of heavy metal pollution at home and abroad, the present invention provides a joint treatment of lead ion pollution method

Method used

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  • Method for joint treatment of lead ion pollution by using carbonate-mineralization microbe-thiobacillus denitrificans
  • Method for joint treatment of lead ion pollution by using carbonate-mineralization microbe-thiobacillus denitrificans

Examples

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Effect test

Embodiment 1

[0016] Example 1 A method of utilizing carbonate mineralizing bacteria-Thiobacillus denitrificans to jointly treat lead ion pollution, applying compound fertilizers containing urea and ammonium thiosulfate to a lead concentration of 50 mg / kg of soil, after 1 day, the concentration of the prepared bacteria was 0.5×10 8 ~5.0×10 8 cfu / mL of carbonate mineralizing bacteria solution according to 0.5L / m 2 The proportion of the above soil was added to keep the temperature range between 20°C and 30°C. After 1d, press 1.0L / m 2 The ratio of adding the prepared strain concentration is 0.5×10 8 ~5.0×10 8 cfu / mL of Thiobacillus denitrificans, use the system of carbonate mineralizing bacteria-Thiobacillus denitrificans to interact with lead ions for 7 days, sample 10mL and centrifuge at 4000r / min for 10min, take 1.0mL of supernatant, and dilute it for testing The residual lead ion concentration in the soil, the final removal rate of lead ion is 90%.

Embodiment 2

[0017]Example 2 A method of utilizing carbonate mineralizing bacteria-Thiobacillus denitrification to jointly treat lead ion pollution, applying compound fertilizers containing urea and ammonium thiosulfate to a lead concentration of 100 mg / kg of soil, after 2 days, the concentration of the prepared strains will be 0.5×10 8 ~5.0×10 8 cfu / mL of carbonate mineralizing bacteria solution according to 1.0L / m 2 The proportion of the above soil was added to keep the temperature range between 20°C and 30°C. After 2d, press 1.0L / m 2 The ratio of adding the prepared strain concentration is 0.5×10 8 ~5.0×10 8 cfu / mL of Thiobacillus denitrificans, use the system of carbonate mineralizing bacteria-Thiobacillus denitrificans to react with lead ions for 8 days, sample 10mL and centrifuge at 4000r / min for 10min, take 1.0mL of supernatant, and dilute it for testing The residual lead ion concentration in the soil, the final removal rate of lead ion is 94%.

Embodiment 3

[0018] Embodiment 3 A kind of method that utilizes carbonate mineralization bacterium-thiobacillus denitrification to jointly process lead ion pollution, apply the compound fertilizer containing urea and ammonium thiosulfate to lead concentration to be 200mg / kg of soil, after 2 days, the concentration of the prepared strains will be 0.5×10 8 ~5.0×10 8 cfu / mL of carbonate mineralizing bacteria solution according to 2.0L / m 2 The proportion of the above soil was added to keep the temperature range between 20°C and 30°C. After 2d, press 2.0L / m 2 The ratio of adding the prepared strain concentration is 0.5×10 8 ~5.0×10 8 cfu / mL of Thiobacillus denitrificans, use the system of carbonate mineralizing bacteria-Thiobacillus denitrificans to interact with lead ions for 10 days, sample 10mL and centrifuge at 4000r / min for 10min, take 1.0mL of supernatant, and dilute it for testing The residual lead ion concentration in the soil, the final removal rate of lead ion is 95.9%.

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Abstract

The invention discloses a method for joint treatment of lead ion pollution by using carbonate-mineralization microbe-thiobacillus denitrificans. The method includes the steps that nutritional ingredients like fertilizer containing urea and ammonia thiosulfate are applied to soil or a water body; the prepared carbonate-mineralization microbe (0.5*10<8>-5.0*10<8> cfu / mL) is added into the soil or the water body polluted by heavy metal according to the proportion of the soil (0.5-5.0L / m<2>) to the water body being 1:10-1:100; after the carbonate-mineralization microbe is cultivated for 1-5d, the prepared thiobacillus denitrificans with the bacterium concentration being 0.5*10<8>-5.0*10<8> cfu / mL is added according to the volume ratio of the soil (0.5-5.0L / m<2>) to the water body being 1:10-1:100 to be evenly stirred and mixed, and cultivation lasts for 3-15d; and the removal rate of heavy metal Pb2 reaches 83%-99%. The method is low in cost, high in efficiency, easy to implement and broad in application prospect.

Description

technical field [0001] The invention relates to a method for jointly treating lead ion pollution by utilizing carbonate mineralizing bacteria-thiobacillus denitrification, and belongs to the technical field of resources and environment. Background technique [0002] Heavy metal pollution refers to the phenomenon that human activities bring heavy metals into the environment, causing the content of heavy metals in the environment to be significantly higher than its natural background value, and causing ecological damage and deterioration of environmental quality. With the rapid development of the economy, a large amount of waste water is discharged, the accumulation of heavy metals in soil and water sources is intensified, and the pollution of heavy metals is also becoming more and more serious. Heavy metal pollution has the characteristics of not being easily dissolved, not being biodegradable, having bioaccumulation, long incubation period, high treatment cost, slow effect, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C02F3/34C02F101/20
CPCB09C1/10B09C2101/00C02F3/34C02F2101/20
Inventor 代群威邬琴琴韩林宝党政王岩
Owner SOUTHWEAT UNIV OF SCI & TECH
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