The invention belongs to the technical field of microbial pesticides, and provides a microbial organic fertilizer for preventing and treating strawberry anthracnose and application thereof in order tosolve the problems that the prevention and treatment of the strawberry anthracnose is neither ideal enough nor stable enough and can not meet actual requirements.The microbial organic fertilizer is prepared from sesame oil residues, chenopodium wheat straws, lyciumruthenicumresidues, naked oat straws, millet straws, jade powder, magnesium sulfate, sugar cane molasses, a composite microbial agent,egg powder and polyacrylamide according to a certain proportion. The composite microbial agent is prepared by mixing bacteria, fungi and actinomycetesin proportion, wherein the bacteria are the uniform mixing of bacillus velezensis, bacillus aryabhattai, bacillus Zhangzhou, bacillus polymyxa and bacillus firmus in proportion, the fungi are trichodermaharzianum, and the actinomycetes are streptomyceteslilaceous. With the microbial organic fertilizer, the ability of strawberry plants to resist anthrax bacteria is improved, the growth of strawberry root systems is promoted, the quality and yieldof strawberries are improved, the creeping stem propagation coefficient of strawberries is increased, more healthy creeping stem seedlings are obtained, and better economic benefits are obtained.