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Marine streptomyces S187 having wide-spectrum antibacterial activity

A marine Streptomyces, antibacterial activity technology, applied in the direction of bacteria, fungicides, microbial-based methods, etc.

Inactive Publication Date: 2008-11-12
DALIAN UNIV OF TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] In order to solve the existing problems in the field of actinomycetes, the present invention utilizes marine microbial resources to provide a strain of marine streptomyces with broad-spectrum antibacterial activity, the marine streptomyces The mold has good antibacterial activity against Staphylococcus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger and Bacillus subtilis, etc.

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  • Marine streptomyces S187 having wide-spectrum antibacterial activity
  • Marine streptomyces S187 having wide-spectrum antibacterial activity
  • Marine streptomyces S187 having wide-spectrum antibacterial activity

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Embodiment Construction

[0014] One, the working steps of isolating marine Streptomyces S187 and studying it are as follows:

[0015] Step 1: Separation of strains

[0016] In August 2006, sea mud samples were obtained from the waters of Xiaoping Island in Dalian at a depth of 20 meters. The samples were stored in ice boxes and brought back to the laboratory for processing. The sample adopts the method of dispersion and differential centrifugation (DDC), adopts oligonutrient medium M5 as the isolation medium, and cultures it at 28°C for one week to isolate marine Streptomyces S187, and uses Bennett's medium as the purification medium.

[0017] Step 2: Antimicrobial Activity

[0018] Antibacterial activity assay medium: nutrient broth agar for bacteria, malt juice agar for Candida albicans, and Chapeauer agar for mold. The bacteria were cultured at 37°C for 24 hours, and the fungi were cultured at 32°C for 48 hours. The antibacterial activity was determined by the agar block method and the fermentati...

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Abstract

The invention relates to marine streptomyces S187 having broad-spectrum antibacterial activity, belonging to the microbiological actinomycetes field. The marine streptomyces S187 is separated from a sea mud sample at a depth of 20 meters underwater in coastal waters. Primary screening through an agar block method proves that the bacterial strain has very strong antibacterial activity to Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger; the result of fermentation liquid secondary screening confirms good activity for antagonizing the Escherichia coli, the Staphylococcus aureus and the Bacillus subtilis. The analysis for 16S rDNA shows that the only actinomycetes having the homology as high as 99 percent with the marine streptomyces S187 is the salt lake bacterial strain Streptomyces chungwhensis (DSM41483) separated by Korean scholars, but the bacterial strain is separated from the sea mud sample for the first time. I type polyketide synthase (PKSI) is successfully amplified out from the streptomyces S187, which indicates that the streptomyces S187 has the potential ability of generating polyketide.

Description

technical field [0001] The invention relates to a strain of marine streptomyces S187 with broad-spectrum antibacterial activity, which belongs to the field of actinomycetes in microbiology. Background technique [0002] Actinomycetes are a class of high (G+C)% Gram-positive bacteria, which have attracted widespread attention due to their unique ability to synthesize a variety of secondary metabolites with complex structures. Many secondary metabolites of actinomycetes have medicinal and plant protection applications, and have been widely used as antibacterial, antifungal and antitumor drugs. About 70% of the tens of thousands of biologically active substances derived from microorganisms that have been discovered are synthesized by actinomycetes. [0003] However, with the decrease of new natural active substances discovered from soil actinomycetes and the continuous production of resistant bacteria, marine actinomycete resources have become a new source for discovering new ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
Inventor 赵心清矫文策袁文杰
Owner DALIAN UNIV OF TECH
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