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Agrobacterium tumefaciens SCUEC1 strain as well as screening method and application thereof

A technology of Agrobacterium tumefaciens and strains, applied in the field of nicotine degradation, can solve the problems of field tobacco leaves that have not been confirmed by experiments, and achieve the effect of accelerated maturation and strong degradation ability

Inactive Publication Date: 2014-08-20
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0004] Chinese invention patent applications (application numbers: 200710013068.3, 200710013069.8, 200710070805.3, 200910097536.9, etc.), the bacterial strains involved in these patents have effective degradation performance on nicotine, and the only disadvantage is that nicotine degradation is carried out under indoor laboratory conditions. Whether real field tobacco leaves have any effect has not been confirmed experimentally

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  • Agrobacterium tumefaciens SCUEC1 strain as well as screening method and application thereof
  • Agrobacterium tumefaciens SCUEC1 strain as well as screening method and application thereof

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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0016] The Agrobacterium tumefaciens SCUEC1 strain provided in this example is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M2014133, and the 16S nucleotide sequence of the strain is shown in SEQ ID NO.1 , including 1189 bases.

[0017] The screening and isolation method of Agrobacterium tumefaciens SCUEC1 provided in this example is as follows: (1), enrichment culture: put 2 g of the soil in the tobacco planting field into 20 mL of nicotine medium, and incubate at 30° C. Use a 180rpm shaker for cultivation. After culturing for 48 hours, transfer 1 mL of the enrichment solution to fresh nicotine medium to continue the cultivation. Repeat the culture transfer step three times to achieve the purpose of enrichment, and collect the enrich...

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Abstract

The invention provides an agrobacterium tumefaciens SCUEC1 strain. The collection number of the strain is CCTCC NO:M2014133, and the nucleotide sequence of the strains is as shown in SEQ ID NO. 1. The strains not only can be applied to nicotine degradation, but also can accelerate the maturity of tobacco leaves. Meanwhile, the invention further provides a method for screening and separating the strains. The method comprises the following steps: performing enrichment culture, then performing separation purification, inoculating separated and purified strains to a nicotine culture medium according to a single colony, culturing in a shaking table for 1-3 days at room temperature, measuring the nicotine content of a fermentation broth of the nicotine culture medium, and secondarily screening the strains according to the magnitude of the nicotine degradation rate to obtain the agrobacterium tumefaciens SCUEC1 strains.

Description

technical field [0001] The invention relates to a microbial strain for degrading nicotine, in particular to an Agrobacterium tumefaciens SCUEC1 strain, a screening method and application of the strain, and belongs to the technical field of nicotine degradation. Background technique [0002] Nicotine is not only the most important chemical component present in tobacco, but the level of nicotine content plays a very important role in the quality of tobacco products. At present, the nicotine content of flue-cured tobacco in my country is generally high, especially in the upper tobacco leaves. For example, the appearance quality of tobacco leaves in some areas around Shennongjia is close to the domestic and international advanced level, but the nicotine content of tobacco leaves in some tobacco-producing counties is as high as 3.5% to 4.5% %, and the nicotine content in the upper part of Burley tobacco is as high as 6%. This not only affects the quality of the upper tobacco, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20A01N63/00A01P21/00C12R1/01
Inventor 李晓华孔文郭利李阳杨振飞王晓丽何冬兰程国军刘涛李开曹丽君陈涛龚传清李永辉曹祥练
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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