954 results about "Agrobacterium tumefaciens" patented technology

Provided is a method of producing D-psicose using a D-psicose epimerase derived from Agrobacterium tumefaciens. Provided are a protein having an amino acid sequence of SEQ ID NO:1 and having a psicose 3-epimerase activity, a gene encoding the protein, a recombinant expression vector containing the gene, and a method of producing D-psicose by reacting the protein produced on a mass scale with D-fructose. The method of producing D-psicose is an environmentally friendly method using a new enzyme, in which an inexpensive substrate is used, and the activity of the enzyme can be retained for a prolonged time period. Thus, the method can be efficiently used for the mass production of D-psicose.

The invention relates to a recombination gene expression system of mortierella alpina (Mortierella alpina ATCC 32222) and applications thereof. According to the invention, by taking mortierella alpina ATCC 32222 uracil auxotroph strains as materials, a set of recombination gene expression system of mortierella alpina is constructed through carrying out genetic manipulation by using an agrobacterium tumefaciens mediated genetic manipulation technique; and an operation is operated by using the system, so that multiple high-yield polyunsaturated fatty acid mortierella alpina strains are obtained, therefore, the recombination gene expression system of mortierella alpina and construction method and applications thereof disclosed by the invention are of great importance in the basic theory study and product development of oil-producing fungi mortierella alpina ATCC 32222.

The invention provides a preparation method of a non-transgenic mutant based on a CRISPR-Cas9 technology. Specifically, the preparation method comprises a construction method and a screening method. The construction method is characterized in that CRISPR-Cas9 and sgRNA are preassembled and are located at T-DNA of agrobacterium tumefaciens; site-directed change of a target gene of a target plant can be realized by infection of the target plant by the agrobacterium tumefaciens and coculture without integrating the sequences of the CRISPR-Cas9 and the sgRNA into a genome of the target plant, so that an obtained site-directed mutant material of the target gene does not need the processes of sexual reproduction, segregation posterity, population screening and the like or does not contain any exogenous gene sequence. The obtained regeneration seedlings are screened by a high-throughput sequencing and high-resolution melting curve technology provided by the invention; mutant plants of which target genes are mutated can be obtained by identifying the regeneration seedlings efficiently and quickly at the current generation of transgene, even if low-proportion mutants of which the proportionis 1 / 100 of a mixed population can be screened. The screening method provided by the invention still can ensure excellent accuracy and sensitivity.

The present invention relates to a method of successively producing D-psicose from D-fructose or D-glucose by using a psicose-epimerase derived from Agrobacterium tumefaciens which is expressed in a food safety form.

The invention consists in modifying the levels of accumulation and emission of monoterpenes and sesquiterpenes in citrus as a mechanism to achieve systemic resistance against pathogens or repellency against pests. The alteration of the content of d-limonene and other terpenes is achieved by genetic transformation via the introduction of a gene that encodes an enzyme with d-limonene synthase activity, from a citrus fruit or plant or from another living organism, in antisense or RNAi (RNA interference) configuration. Genetic modification is achieved either by Agrobacterium tumefaciens or any other method of genetic transformation of plants from protoplasts or explants. The construction is incorporated in citrus genotypes or related genera of the family Rutaceae in order to reduce the levels of accumulation and emission of the monoterpene and precursor compounds and / or derivatives, either of leaves or flowers and / or fruit.

The invention discloses a method for increasing artemisinin content of sweet wormwood by transferring AaWRKY1 gene. The method includes the steps of cloning WRKY type transcription factor AaWRKY1 gene in sweet wormwood; constructing a plant expression vector containing the AaWRKY1; introducing the AaWRKY1 gene into the sweet wormwood to regrow a plant under mediation of Agrobacterium Tumefaciens; performing PCR (polymerase chain reaction) to detect integration status of the foreign target gene AaWRKY1; measuring the content of artemisinin in the transgenic sweet wormwood by efficient liquid chromatography and an evaporative light scattering detector, and screening to obtain the plants of sweet wormwood with artemisinin content increased. The content of artemisinin in the transgenic sweet wormwood with the AaWRKY1 gene is increased to 24.59mg / g DW by the method and is 4.44 times of that of the non-transgenic sweet wormwood. The method provides new high-yield and stable drug sources for large-scale production of artemisinin.

The invention relates to a mortierella alpina, M. alpina genetic engineering strain of an overexpression omega 3 desaturase gene (FADS15) and a construction method of the strain. According to the invention, a mortierella alpina, M. alpina uracil nutritional-deficient strain MAU1 (CCFM501) is taken as a material, an agrobacterium tumefaciens-mediated genetic manipulation technology is applied to obtain an omega 3 desaturase (omega 3Des) overexpression strain of high-yield EPA (eicosapentaenoic acid), and the strain and the construction method have important significance on basic theoretical research and product development of mortierella alpina, M. alpine as an oil-producing fungus.

The present invention relates to a method of successively producing D-psicose from D-fructose or D-glucose by using a psicose-epimerase derived from Agrobacterium tumefaciens which is expressed in a food safety form.

The procedure is based on the use, as vegetable starting material for the transformation, of the first shoots from the graft of buds of adult trees onto stock, the genetic transformation of explants from the adult shoots by mean of co cultivation with Agrobacterium tumefaciens in mother plaques, and the obtaining of complete adult woody plants by means of in vitro micrografting of the transgenic buds, apices or shoots, regenerated by means of the explants, by means of in vitro micrograft onto stock cultivated in vitro. This procedure makes it possible to avoid the juvenile period and the high heterozygosis which affects most woody species, blossoming and fruiting of the transgenic plants are brought forward, and it permits the direct genetic transformation of commercially interesting varieties. It has argicultural applications.