117 results about "Mortierella alpina" patented technology

Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C₂₀ in oleaginous yeast, such as Yarrowia lipolytica.

The invention belongs to the field of fermentation engineering, relates to a method for producing ARA (arachidonic acid) through microbial fermentation, and particularly relates to a method for producing compound fat containing the ARA through industrial fermentation of a mortierella alpine strain. More particularly, the invention relates to a method for culturing microorganisms for producing the ARA, wherein the method for culturing the microorganisms for producing the ARA comprises the steps of controlling the fermentation temperature to be 20 to 25 DEG C after culturing in a fermentation tank for 100 to 140h; and/or, stopping ventilation or reducing the ventilatory capacity by not less than 50 percent at the 8th to 10th day of the culturing in the fermentation tank. The method for preparing the ARA has the advantages that the yield is high, the purity is high, and the large-scale industrial production of the ARA is facilitated.

The invention discloses a technology for extracting arachidonic acid oil by an aqueous enzymatic extraction. The technology comprises the following steps: using Mortierella alpina as an original strain, fermenting and culturing, diluting by adding distilled water to obtain a thallus suspension, regulating the pH value of the suspension, adding a biological enzyme, oscillating at constant temperature for 6-10 hours, adding ethanol and normal hexane to carry out oil extraction, centrifugating to delaminate, taking out an organic solvent phase, adding normal hexane into the left water phase and solid phase to carry out secondary extraction, centrifugating to delaminate, combining with the organic solvent phase, and carrying out rotary evaporation on the organic solvent phase to obtain the oil and an organic solvent. The invention has the advantages of low energy consumption, low cost, high yield of arachidonic acid oil, high quality, simple reactor, easy batch large-scale application and the like.

The invention discloses a method for preparing an ARA (Arachidonic Acid) by culturing a mortierella alpina by utilizing a solid material culture medium. The method comprises the following steps of using rice bran and niblet as material, adding inorganic salt in defined amount, using the inorganic salt as the solid material culture medium, collecting and smashing thallus after culturing strain in liquid, inoculating smashed thallus to the solid material culture medium, controlling culture temperature, mosturizing and controlling relative humidity during a culture process and appropriately stirring, and generating a large amount of spores. pH (Potential Of Hydrogen) segmentation regulation and control can be carried out during a fermentation process by using a citric acid and citrate. According to the method disclosed by the invention, the amount of seed spores of is large, the sunchronism is good, the activity is strong, the fermentation period of the mortierella alpina can be shortened, the growth of the mortierella alpine and the accumulation of ARA grease can be facilitated through the pH segmentation regulation and control, and the production and the quality of the ARA can be increased.

The invention discloses a mortierella alpine and the application thereof. Bacterial strains are conserved in China Center for Type Culture Collection (CCTCC) with a CCTCC NO. M 207067. The output of arachidonic acid produced by the bacterial strains upon fermentation can be up to 10g/l and the yield is up to 1.54g/l/d, thereby, the bacterial strains is applicable to industrial production of the arachidonic acid.

The invention provides a technology for preparing grease containing rich polyunsaturated fatty acid by taking lignocellulose type agricultural and forest wastes as raw materials through fermenting mortierella alpine, aiming at the problems in the prior art. The technology comprises the steps of pre-treating, saccharifying, carrying out mortierella alpine fermentation, extracting the grease and thelike. According to the technology provided by the invention, a fermentation phase of the mortierella alpine adopts a temperature gradual reduction method and the yield of ARA (Arachidonic Acid) can be remarkably improved; the ratio of the ARA in the total grease can reach 48 percent. Compared with the prior art, the technology adopts a cellulose preparation based on fiber-production globule bacteria to realize the saccharifying of lignocellulose, so that the cost of a carbon source and the enzyme utilization cost of a saccharifying phase are remarkably reduced; the cellulose saccharifying efficiency is 80 to 90 percent. Furthermore, a culture medium at a lignocellulose saccharifying phase and a fermentation culture medium in the technology can be circularly utilized, so that water can beremarkably reduced and the dosage of chemicals also can be remarkably reduced; the technology has remarkable effects of reducing wastewater emission and reducing the cost.

A preparation method of arachidonic acid relates to the arachidonic acid. The method is as follows: optimizing culturing conditions of mortierella alpina arachidonic (ARA), fermenting in batches on basal media, then fermenting in batches on the optimized media by means of using yeast powder and corn steep liquor as nitrogen source, performing a fermenter enlarging experiment by using the yeast powder as the nitrogen source, culturing the mortierella alpina on the optimized media by using the corn steep liquor and the yeast powder as the mixed nitrogen source, and obtaining the mixed nitrogen source optimized media by replacing the nitrogen source on the optimized media into the corn steep liquor and the yeast powder; fermenting the mortierella alpina in batches on the mixed nitrogen source optimized media; performing high-glucose acclimatization to the mortierella alpina and fermenting the acclimated strains in batches on the mixed nitrogen source media; when the concentration of the glucose is reduced to 10g/L, performing fed-batch operation by 600 g/L of glucose so as to control the residual glucose within about 10 g/L, and starting to harvest dry strains, grease and the ARA after culturing for 168 h.

The invention discloses a decomposition maturing agent and the application thereof to green waste recycling. A fermentation broth is combined by adopting 20 to 50 parts of streptomyces griseus, 5 to 15 parts of pholiota adipose, 5 to 15 parts of mortierella alpine, 5 to 15 parts of flavobacterium columnare, 5 to 15 parts of pseudomonas stutzeri and 20 to 40 parts of rhodopseudomonas palustris; the bacterial broth is fully adsorbed on a carrier, namely crushed green waste of which the particle size is 1 to 30mm; water is added to the green waste until the water content reaches 40 percent to 70 percent, and then 100 to 300g of decomposition maturing agent are added to the green waste per cubic meter according to a volume ratio; auxiliary materials are urea and calcium superphosphate, and the weight ratio of the urea to the calcium superphosphate is 1:3; the weight ratio of the carrier to the auxiliary materials is 1:2; the decomposition maturing agent is obtained after the fermentation broth, the carrier and the auxiliary materials are evenly mixed; the decomposition maturing agent and the green waste are composted and fermented in a sealed manner at a temperature of 20 to 40 DEG; compost is turned once every 30 days, and water is supplemented to the compost until the water content reaches 40 to 70 percent when the compost is turned; and after being composted for 120 days, the green waste is spread and aired to be dried, and then organic fertilizer is obtained. The decomposition maturing agent has a wide application value.

The invention relates to a new separated bacterial strain of mortierella alpine (Mortierella Alpina) and an application thereof. Particularly, the invention relates to CGMCC No.6254, offspring thereof, culture and/or mutant strain and a method and application in preparing arachidonic acid by using the bacterial strain, offspring thereof, culture and/or mutant strain. The separated bacterial strain is a natural bacterial strain, and can stably produce arachidonic acid with a yield as high as 14.32g/L.

The invention relates to a method for promoting production of mortierella alpina and oil yield of arachidonic acid. The method comprises the following steps that fermented clear liquid of the mortierella alpina is collected, and through extraction and concentration of hydrophilic organic solvent, an accelerating agent which can adjust and control the production of the mortierella alpine and the oil yield of the arachidonic aicd is obtained by screening; and in the 0-48 hours at the early stage of fermentation, the accelerating agent is added to a normal medium according to the ratio of 0.1-0.5%, and a conditioned medium is prepared to inoculate and cultured at the temperature of 28 DEG C. After the fermentation, in fermentation results of some embodiments, biomass is 43.2 g/L, oil content is 61.3%, ARA content is 60.7%, ARA yield is 16.1 g/L, and the fermentation period is shortened from 192 hours to 168 hours. The method for promoting the production of the mortierella alpina and the oil yield of the arachidonic acid is simple and effective, promotes the production of the mortierella alpine, improves the oil yield of the arachidonic acid, and is of great significance to industrial application.

The invention discloses a mortierella alpine mutant strain, a method for producing arachidonic acid oil by using the same and arachidonic acid oil. The mortierella alpine mutant strain (Mortierella alpine Y16) is preserved in the China Center for Type Culture Collection (CCTCC) on July 2, 2015, the preservation address is Wuhan University in Wuhan City of China, and the preservation number is CCTCC NO: M2015421. In the arachidonic acid oil produced by using the mortierella alpine mutant strain, the content of triglyceride is more than or equal to 92wt%, the content of arachidonic acid is at least 48wt%, and the content of long-chain saturated fatty acid with more than 20 carbons is lower than 6.3wt%.