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Method for producing arachidonic acid grease by utilizing mortierella alpina mutant strain, and arachidonic acid grease produced by method

A technology of arachidonic acid and Mortierella alpine, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reducing the content of long-chain saturated fatty acids, not mentioned, etc. Guaranteed quality, high-quality results

Active Publication Date: 2014-02-12
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the present invention does not mention how to reduce the content of long-chain saturated fatty acids

Method used

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  • Method for producing arachidonic acid grease by utilizing mortierella alpina mutant strain, and arachidonic acid grease produced by method
  • Method for producing arachidonic acid grease by utilizing mortierella alpina mutant strain, and arachidonic acid grease produced by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1. The breeding method of Mortierella alpina mutant strain

[0029] (1) Commercially available Mortierella alpina was used as the starting strain.

[0030] (2) Inoculate the strains on potato dextrose agar (PDA) medium and culture them at a constant temperature of 28°C for 8 days until the spores mature.

[0031] (3) Filter the pure spore liquid through gauze or filter paper, pour the spore liquid into a sterile culture dish and irradiate it with an ultraviolet lamp. The irradiation distance of the ultraviolet lamp is 30 cm, the irradiation time is 60 seconds, and the power of the ultraviolet lamp is 30 watts. .

[0032] (4) The spore liquid after ultraviolet mutagenesis is dried with sterile air to become bacterial plaque. Aseptically move the petri dish containing bacterial plaque into a high-energy particle beam implanter, and undergo mutagenesis by high-energy ion beam implantation.

[0033] (5) The plaques were eluted with sterile water, diluted, spre...

Embodiment 2

[0040] Embodiment 2 utilizes Mortierella alpina mutant strain to produce arachidonic acid

[0041] a) Preparation of spore suspension: Inoculate commercially available Mortierella alpina and the mutant strain of Mortierella alpina with the preservation number of the present invention: CCTCC M2013419 on a potato dextrose agar (PDA) medium plate, 25-28 Cultivate at ℃ for 7 days until the spores mature, and then scrape the spores and hyphae on the potato dextrose agar (PDA) medium plate to fill with 10 ml of sterile water, and shake to obtain a spore suspension.

[0042] b) Seed shake flask culture: inoculate the spore suspension in step (1) into the seed bottle with the culture medium, the inoculum amount is 10% (volume ratio), and place it on a shaker at 25°C and 120 rpm for 36 hours, the medium is: carbon source glucose 20g / l; nitrogen source yeast powder 5g / l; pH 5.5.

[0043] c) Fermentation bottle culture: When the bacterial concentration in the seed bottle reaches 15% (vo...

Embodiment 3

[0049] Embodiment 3 utilizes Mortierella alpina mutant strain to produce arachidonic acid

[0050] a) Preparation of spore suspension: take commercially available Mortierella alpina and the Mortierella alpina used in the present invention (preservation number: CCTCC M2013419) and inoculate them on potato dextrose agar (PDA) medium plates at 25-28°C Cultivate for 8 days until the spores mature, then scrape the spores and hyphae on the potato dextrose agar (PDA) medium plate to fill with 20 ml of sterile water, and shake to obtain the spore suspension.

[0051] b) Shake flask seed culture: inoculate the spore suspension in step (1) into the seed bottle with the medium, the inoculum amount is 15% (volume ratio), and place it on a shaker at 28°C and 220 rpm for 48 hours, the medium is: carbon source sucrose 35g / l; nitrogen source yeast extract powder 12g / l; pH7.

[0052] c) Expanded seed cultivation: the final fermenter culture adopts a volume of 50 L, so the seed tank is selecte...

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Abstract

The invention relates to a method for producing arachidonic acid grease by utilizing a mortierella alpina mutant strain, and the arachidonic acid grease produced by the method. The method comprises the steps that the mortierella alpina mutant strain is fermented; a fermentation product is collected; aftertreatment is performed; the arachidonic acid grease is obtained. The mortierella alpina mutant strain (mortierella alpina ZR36) is preserved in China Center For Type Culture Collection (CCTCC) on September 13, 2013; the preservation address is Wuhan University, Wuhan City, China; the preservation number is CCTCC NO:M2013419. The total content of saturated fatty acid, particularly above eicosanoic long-chain saturated fatty acid in the arachidonic acid grease produced by the mortierella alpina mutant strain is relatively low (lower than 15wt%); a freezing point of the arachidonic acid grease is relatively low (low to 4 DEG C); the arachidonic acid grease cannot be frozen or crystallized even if at a lower temperature; saturated fatty acid cannot be separated out; the arachidonic acid grease can keep clear and transparent, and has higher quality.

Description

technical field [0001] The invention relates to a method for producing arachidonic acid oil by using Mortierella alpina mutant strain and the produced arachidonic acid oil. Background technique [0002] There are many kinds of unsaturated fatty acids in microbial oil, and unsaturated fatty acids mainly include monounsaturated fatty acids and polyunsaturated fatty acids, which are of great benefit to human health. Polyunsaturated fatty acids can synthesize docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA), which have the functions of lowering blood fat, improving blood circulation, inhibiting platelet aggregation, It has the effects of inhibiting atherosclerotic plaque and thrombus formation, and also has a good preventive effect on cardiovascular and cerebrovascular diseases. [0003] Arachidonic acid oil has entered the stage of industrial production. However, most of the current research only focuses on the content of arachidonic acid in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 汪志明余超陆姝欢江旭伍维
Owner CABIO BIOTECH WUHAN CO LTD
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