Method for producing ARA (arachidonic acid) through microbial fermentation
A technology of microorganism and fermenter is applied in the field of industrial fermentation of Mortierella alpine strain to produce mixed oil containing arachidonic acid, which can solve the problems of polluted environment, long high temperature treatment time, increase of product peroxide value and anisidine value, etc.
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Embodiment 1
[0123]The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to ...
Embodiment 2
[0129] The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to...
Embodiment 3
[0135] The slant-preserved strain of Mortierella alpine was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 28° C. at a rotational speed of 150 rpm for 48 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, connect the shake flask seed liquid into the first-level seed tank equipped with the sterilized medium, and cultivate it for 30 hours under the conditions of the culture temperature of 28°C, the ventilation volume of 1vvm, and the tank pressure of 0.04MPa, and the first-level is completed. Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and cultivated for 24 hours under the conditions of a culture temperature of 28 ° C, an air volume of 1 vvm, and a tank pressure of 0.04 MPa. Complete secondary seed expansion cultivation. According to...
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