Method for producing grease containing rich polyunsaturated fatty acid by adopting lignocellulose

Abstract

The invention provides a technology for preparing grease containing rich polyunsaturated fatty acid by taking lignocellulose type agricultural and forest wastes as raw materials through fermenting mortierella alpine, aiming at the problems in the prior art. The technology comprises the steps of pre-treating, saccharifying, carrying out mortierella alpine fermentation, extracting the grease and thelike. According to the technology provided by the invention, a fermentation phase of the mortierella alpine adopts a temperature gradual reduction method and the yield of ARA (Arachidonic Acid) can be remarkably improved; the ratio of the ARA in the total grease can reach 48 percent. Compared with the prior art, the technology adopts a cellulose preparation based on fiber-production globule bacteria to realize the saccharifying of lignocellulose, so that the cost of a carbon source and the enzyme utilization cost of a saccharifying phase are remarkably reduced; the cellulose saccharifying efficiency is 80 to 90 percent. Furthermore, a culture medium at a lignocellulose saccharifying phase and a fermentation culture medium in the technology can be circularly utilized, so that water can beremarkably reduced and the dosage of chemicals also can be remarkably reduced; the technology has remarkable effects of reducing wastewater emission and reducing the cost.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for cultivating Mortierella alpina by using lignocellulose raw materials to produce oil rich in arachidonic acid and other polyunsaturated fatty acids. Background technique [0002] Mortierella alapina (Mortierella alapina) is a filamentous fungus of the genus Mortierella in the family Mucorales, which is widely distributed in soil, feces and other decaying organic substances. It is currently the fungus that produces arachidonic acid. The only strain with a formal safety assessment (GRAS). Because it can accumulate a considerable proportion of arachidonic acid (ARA or AA), Mortierella chrysogenum, which belongs to the same genus Mortierella, has received widespread attention and is recognized as an excellent strain for arachidonic acid production. Arachidonic Acid (ARA or AA for short) is an essential omega-6 long-chain polyunsaturated fatty acid that m...

AI Technical Summary

Problems solved by technology

However, since the activity and stability of exogenously added proteins will decrease with the progress of the saccharification process, the consumption of enzymes requires an increase in the amount or frequency of enzyme addition

In addition, these non-cellulosomal proteins are added to the saccharification system as free enzymes, which cannot interact with cellulosomes, and will significantly reduce the synergistic effect with other enzymes in the system, inevitably resulting in additional enzymes that are greater than the demand, resulting in The high cost of enzyme preparations

Not only that, this saccharification strategy that relies on the addition of exogenous free enzymes has complex processes, high equipment requirements, and conversion efficiency that cannot meet the requirements of industrial production.

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