Aiming at the problems of cellulosic saccharification in lignocellulose saccharification in the prior art, the present invention provides a whole-bacteria enzyme preparation for catalyzing lignocellulose saccharification. A whole-bacteria enzyme preparation for catalyzing lignocellulose saccharification, said whole-bacteria enzyme preparation interacts with components in cellulosomes through non-cellulosome proteins, and binds non-cellulosome proteins to cellulosome complexes In the process, the cellulosome-modified bacterial strain is obtained; then the bacterial strain is used for saccharification, that is, the saccharification process involves the participation of bacterial cells. The whole-bacteria enzyme preparation described in the present invention not only realizes the maintenance of its stability and activity, but also greatly improves its synergistic effect with other enzymes in the system, thereby reducing the consumption of enzymes, reducing the cost of production and the cost of the process. cumbersome.