48 results about "Multienzyme complexes" patented technology

The invention discloses a recombinant bacillus subtilis and a construction method and an application thereof, which belong to the technical field of genetic engineering. The method takes cell self functional membrane microdomain FMMs as a space frame, and constructs a multienzyme complex by using the specific marker proteins FloA and FloT, an artificial substrate channel is constructed, the metabolic burden of the cells can be effectively reduced, and the multienzyme complex is attached to plasmalemma , which facilitates the transport of a product from the intracellular to the extracellular. The recombinant bacillus subtilis constructed by the method efficiently synthesizes GlcNAc without affecting cell life activities, and a toxic intermediate metabolite GlcN-6-P can also be confined to the vicinity of the plasmalemma to reduce or eliminate the inhibition of cell viability. During a shake flask fermentation of a composite medium, the yield of acetylglucosamine in contrast bacterial strain BSGC is only 0.45 g / L, and the BSGAT acetylglucosamine output is increased to 5.29g / L, which is 11.76 times that of the contrast bacterial strain. The recombinant bacillus subtilis construction method of the invention is simple and convenient to use, and has the good application prospect.

A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.

The invention relates to a preparation method of a hydrolyzed protein mixture and a protein peptide powder feed additive, wherein a multienzyme complex enzyme method is used for hydrolyzing vegetable protein to prepare the hydrolyzed protein mixture and protein peptide powder. The technology of the method is simple, and a non-starch polysaccharide enzyme and a proteinase are compounded so as to be capable of improving the hydrolyzation rate and degree of protein as well as the utilization rate of feed and reducing the production cost. Products prepared by the preparation method can be used asthe feed additive to be capable of significantly improving the laying rate of egg-laying poultry, improving the weight increment of meat poultry, reducing the feed-meat rate and improving the production performance of table poultry as well as reducing the incidence rate of diarrhea in young animals and the feed-meat rate of piggy; and the preparation method is applied to aquatic livestock to be capable of improving the weight increment of fish and reducing the bait coefficient and content viscosity in the intestinal tract.

This invention describes novel catalytically active cytosolic enzymes for triacylglycerol biosynthesis from eukaryotic systems. The complex from oleaginous yeast was enzymatically characterized, and was found to contain lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, acyl—acyl carrier protein synthetase, superoxide dismutase and acyl carrier protein. The triacylglycerol biosynthetic machinery rapidly incorporates free fatty acids as well as fatty acyl-coenzyme A into triacylglycerol and its biosynthetic intermediates. Lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase and diacylglycerol acyltransferase from the complex were microsequenced. Acyl carrier protein, superoxide dismutase and diacylglycerol acyltransferase genes were cloned and expressed in bacterial system. The soluble triacylglycerol biosynthetic enzymes (lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase) in yeast, rat adipocytes and human hepatocyte cell-line (HepG2) exist in the cytosol either as free enzymes or as a multienzyme complex.

An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.