202 results about "Enzyme complex" patented technology

The present invention provides a simple and rapid method for preparing purified transposase complexes that are highly suited for fragmenting DNA. The method includes forming transposase complexes with oligonucleotide adapters in cell lysate, then purifying the complexes from the other substance in the cell lysate. Purification is accomplished using a specific binding pair, in which one member of the pair is bound to an oligonucleotide adapter of the complex and the other member of the pair is bound to a solid substrate. The bound complexes can be immediately used in DNA fragmentation reactions to produce solid substrate-bound DNA fragments, which can be used for any number of purposes, including as templates for amplification and sequencing.

The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid.

The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid.

The present invention provides for stable nicotinamide adenine dinucleotide (NAD / NADH) and nicotinamide adenine dinucleotide phosphate (NADP / NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.

A method for recycling cellulase enzymes. Also provided is a method for producing fermentable carbohydrates, plant leaf protein, and lignin, by adding a cellulase enzyme complex expressed from and on irradiated cellulase complex-producing organisms with sufficient radiation to kill biological activity without destroying all cellulase enzyme complex activity to biomass. The fermentable carbohydrates produced by the method. Also provided are irradiated cellulase-producing organisms for use in converting biomass to fermentable sugars, plant leaf protein, and lignin. A method for producing cellulase enzymes for glucose and other sugar production and protein and lignin extraction by irradiating cellulase-producing organisms, thereby producing the cellulase enzymes is also provided. A system for producing fermentable carbohydrates, plant protein, and lignin, said system comprising irradiated cellulase-producing organisms and biomass is provided.

The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid.

The invention relates to a method for detecting thrombin with an electrochemical aptamer sensor based on aptamer-gold nanoparticle-horseradish peroxidase signal amplification, belonging to the technical field of analytical chemistry, characterized in that: a thrombin aptamer I is fixed on the gold coated magnetic nanoparticles as a capture probe, an aptamer II is double-labeling by gold nanoparticle and horseradish peroxidase to form an aptamer-gold nanoparticle-horseradish peroxidase composite as a detection probe; the capture probe and the detection probe are combined with thrombin to form a [gold coated magnetic nanoparticle-aptamer I] / thrombin / [ aptamer II-gold nanoparticle-horseradish peroxidase] sandwich structure; and the sandwich structure is fixed on the surface of the electrode, and thrombin is detected through enzyme catalytic reaction. The method has the advantages of simple operation, rapid response, high sensitivity, and strong specificity. The detection limit of thrombin is 30 fmol.L<-1>, and the linear range is 0.1-60 pmol.L<-1>.

Disclosed herein are analogs of Salinosporamide A, having the Formulae Ia-IVa as follows: Like Salinosporamide A, the compounds of the present invention will inhibit the proteasome, an intracellular enzyme complex that destroys proteins the cell no longer needs. Without the proteasome, proteins would build up and clog cellular machinery. Fast-growing cancer cells make especially heavy use of the proteasome, so thwarting its action is a compelling drug strategy.

A two component composition comprises an anchor enzyme complex to degrade biofilm structures and a second anchor enzyme component having the capability to act directly upon the bacteria for a bactericidal effect.

The invention discloses a bio-enzyme bacteriostatic oil stain-emulsifying preparation prepared from a surfactant, a chelating agent, a rust-proof preservative, triethanolamine, an environmental-protection organic solvent, a bactericide, D-limonene, bio-enzyme, an enzyme stabilizer and deionized water according to a certain weight ratio. The bio-enzyme bacteriostatic oil stain-emulsifying preparation allows the bio-enzyme stabilizer with the bio-enzyme, the surfactant and the bactericide to form a bio-enzyme complex system under a proper acidity and alkalinity condition, has the oil stain emulsification decomposition effect enhanced, has mild and no pungent smell, and provides anti-corrosion protection for light metals aluminum, zinc and the like.

The invention belongs to the technical field of immunologic diagnosis, in particular to a treponema pallidum antibody diagnostic kit by a chemiluminescence method and a preparation method thereof. The kit comprises an anti-TP test reaction plate, an anti-TP test enzyme complex, chemiluminescence substrate liquid, concentrated washing liquor, a negative contrast and a positive contrast. The invention also discloses a preparation method of the diagnostic kit, which adopts a chemiluminescence immunoassay technology; compared with ELISA (enzyme-linked immuno sorbent assay), the method has higher sensitivity and specificity, is suitable for the auxiliary diagnosis of clinical syphilis and screening of blood donors and fills a blank of the production of a treponema pallidum antibody diagnostic reagent detected by the domestic chemiluminescence method.

The invention discloses an aspergillus niger strain and use thereof. The aspergillus niger strain is aspergillus niger A46CGMCC No.3084. The strain can produce xylanase, cellulose, pectinase, amylase, beta-mannase and the like with high yield at the same time of xylanase, cellulose, pectinase, amylase, beta-mannase and the like at the same time, as well as an enzyme complex for feeding animals, wherein the optimum pH for active enzymes in the enzyme complex is between 4 and 6 which meets the requirements of the gastrointestinal tracts of the animals. The enzyme complex for feed use can be widely used in livestock feed to reduce anti-nutritional factors, improve the digestibility, create excellent economic benefits and promote ecological environment protection and the sound development of the livestock industry.

The invention discloses a biological composite enzyme, belongs to the field of biotechnology and particularly relates to a biological composite enzyme product. The product provided by the invention consists of product of culture of aspergillus awamori, lactobacillus plantarum preparation and bacillus subtilis preparation. Each gram of biological composite enzyme contains 4.0 to 9.5*10<8> bacillus subtilis, 4.0 to 8.5*10<7> aspergillus awamori, 150 to 200IU of protease, 200 to 250IU of beta-glucanase, 50 to 150 IU of xylanase, 100 to 120 IU of cellulose and 250 to 300 IU of amylase. When the biological composite enzyme is applied into soil, the soil looseness of an active layer can be promoted, the soil fertility can be improved, and the sound soil environment suitable for the roots of crops to grow. The conversion of the soil bio-enzyme has obvious effects in aspects of reducing agricultural production cost, reducing consumption of fertilizer, restoring ecological productivity of soil, effectively improving crop yield, and the like and can give full play to the efficacy of the organic matters of the soil.

The invention relates to a processing method of edible brown rice and the edible brown rice made by the method. The control starts from paddy which is stored in a cryogenic thermostat warehouse after the drying in a drying tower. Afterward, the paddy was processed into brown rice which is used as raw material by cleaning, stoning, deshelling, rolling by a vertical rice mill, polishing and color-selecting. The brown rice is processed in sequence in the steps as follows: after water flushing, draining, adding with the enzyme complex liquid, the brown rice is immersed in the mixed liquor in an ultrasonic field; the PH of the immersed brown rice is adjusted to neutrality, then the brown rice is cleaned by water; the brown rice which is added with the enzyme complex liquid is cooked in a continuous cooking machine, by which the cooked brown rice is dried in the recirculation ventilation continuous dryer; after microwave sterilizing and subpackaging, the brown rice is formed into the finished products of quick-cooking edible brown rice. The invention more particularly relates to that the edible brown rice has better taste by the control of the processing process, is easier for cooking at home and boiled with the rice simultaneously, and has higher safety for the microbial contamination.

Provided are a multi-enzyme complex preparation containing microcapsule and a preparation method and an application thereof. The preparation method includes placing 20.7%-22.9% of amylase, 8.4%-11.3% of saccharifying enzyme, 7.6%-9.8% of cellulose, 10.9%-13.1% of pectinase, 11.4%-15.3% of catalase, 6.4%-9.7% of laccase, 0.2%-8.5% of enzyme stabilizer and 0.01%-0.5% of preservative by weight into water, stirring the mixture to enable the mixture to be dissolved completely and form water solution, dispersing 8.6%-12.5% of microencapsulation protease into the water solution to obtain the multi-enzyme complex preparation containing the microcapsule. The multi-enzyme complex preparation containing the microcapsule is added into tobacco raw materials in a feeding mode according to 0.5%-1% of the weight of the tobacco raw materials in a tobacco threshing re-baking line or a tobacco storage alcoholization or formula tobacco thread making line. The microencapsulation protease in the multi-enzyme complex preparation contains a high polymer material wall material, the protease wrapped by the wall material is not easy to dissolve in the low water solution state and can be dispersed and dissolved quickly in the diluted high water solution state, so that activity loss of the protease and other enzymes caused by the fact that the protease reacts with other enzymes is avoided.

The invention discloses a preparation method of an amphipathic gama-polyglutanmic acid nanodrug carrier. The preparation method comprises the steps of grafting hydrophobicity group on gama--polyglutanmic acid serving as a main chain, thus obtaining amphipathic gama-polyglutanmic acid derivates with good biocompatibility and degradability, and self assembling in water medium to form nano micelle with particle size of 200-1000nm. The hydrophobic nuclei-hydrophilic shell structure is beneficial to improvement of solubility of hydrophobic drug in water medium, further possibly improves the bioavailability of drugs in the body, and also can be used for load of protein and DNA. The preparation method of the amphipathic gama-polyglutanmic acid nanodrug carrier is simple and practicable, all raw materials can be industrially produced; reproducibility is good; and the amphipathic gama-polyglutanmic acid nanodrug carrier has high drug loading rate, good sustained release effect and intelligent environment response (pH-enzyme complex sensitivity) and other corresponding characteristics, as well as good popularization and application values.

Electrodes carrying FAD-dependent enzymes on their surface are enclosed. The FAD is modified to include a functional group or moiety which affects the properties of the enzyme. The functional group or moiety can be a binding moiety through which the FAD-enzyme complex is immobilized onto the electrode; it can be an electron mediator group for transferring electrons between the electrode and the FAD; or it can be a photoisomerizable group which can undergo photoisomerization which yields a change in the rate of the electrically induced catalytic activity of the enzyme. The electrodes can be used in electrochemical systems for deforming analytes in liquid medium or for recordal of optical signals.

The invention relates to a fabric dirt pretreatment agent containing a steady biological enzyme complex and a low-activity substance and a preparation method thereof. A product of the invention takes fatty alcohol polyoxyethylene ether (7), fatty alcohol polyoxyethylene ether (9), sodium fatty alcohol-polyoxyethyleneether sulfate (70 percent), fatty alcohol polyoxyethylene ether (JFC), polyacrylate, sodium citrate, boric acid, propylene glycol, prolease, calcium chloride, isothiazolinone, essence, and deionized water as raw materials; and the method adopts a polymer to ensure that the prolease forms a polymer complex in a system by researching biological enzyme stabilization technology, which ensures the long-term effective activity of biological enzyme, ensures that the product can achieve stronger detergency when the content of an active substance is 10 percent or so and has steady quality during the storage, and achieves the aims of saving resources and protecting environment. The product is neutral, and has unique advantages in preventing fading, reducing the damage of clothes, protecting skin, and reducing pollution.

The invention discloses a method for making instant green tea powder. In the invention, tannase is produced by fermenting tea stalks, and then compounded with cellulase, papain and pectinase to carry out enzymolysis and extraction of tea leaves to fully extract the tea leaves The macromolecular substances in the tea are decomposed into small molecular substances, so that the nutrients in the tea are fully leached, which is beneficial to the body's absorption, and the tea soup after brewing is not easy to appear "cold and muddy" phenomenon; Damage the cell wall, promote the dissolution of effective substances in the tea, and then carry out vacuum-coupled ultrasonic extraction on the tea after enzymatic hydrolysis, improve the extraction rate of tea polyphenols and other substances in the tea in a short time, and at the same time inactivate the enzyme activity, which can quickly Good to keep the tea fragrance.

The invention belongs to the field of micro-fluidic chips, and relates to a biological macromolecule detection method. An alpha-LISA technology and a droplet-based microfluidics technology are combined and optimized to prepare a micro-fluidic chip liquid. A novel biological macromolecule detection method with a high sensitivity is provided. A three-phase mixing technology is adopted. A passive mixing structure is used to fully and evenly mix the reaction liquid. A cross focusing technology is used to form droplets. The generated droplets have the advantage of smaller volume, and thus the reactions become faster. Moreover, the requirements on the samples are lower, the reactions are specific, sensitive, fast, and full; the washing does not need to be carried out after reactions; complicated complexes such as complete proteins, enzyme complex, bacteriophage, and the like, can be detected; the technical bottleneck of conventional commercial liquid chips at present can be broken through; the provided method can be applied to fast clinical biological macromolecule detection; the detection sensitivity and specificity are both improved, and the required sample amount is reduced.

The invention relates to the field of yeast polypeptides, in particular to a yeast polypeptide and its preparation method and application. The preparation method of the yeast polypeptide comprises the following steps: (1) extracting the yeast protein in the raw material; (2) adding alkaline protease for enzymolysis, then adding compound protease for enzymolysis, collecting the supernatant to obtain the enzymolysis product; (3) ) separation and decolorization to obtain yeast polypeptide. The yeast polypeptide obtained by the present invention is colorless and tasteless, with a polypeptide content of more than 80%, and more than 85% of the polypeptide has a molecular weight of less than 2000, contains almost no free amino acids, and does not have the bitter taste of general soybean peptide and other peptide products, and can be used in food And health care products, which can improve the flavor of food, help restore physical strength after exercise, etc. Moreover, if the yeast polypeptide obtained in the present invention is not dried and powdered, it is an excellent cosmetic essence, which has nourishing and anti-wrinkle activity, can be applied to cosmetics, and does not affect the color, fragrance, etc. of cosmetics, and has anti-aging effects .

An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.

The invention discloses a process for fermenting and producing a feeding enzyme complex preparation by using dextran waste liquid, and the product thereof. The fermentation production process uses strains of aspergillus niger (05012) and strains of bacillus spore subtilis as enzyme-producing strains for production; the dextran production waste liquid is blended and then used as a raw material of liquid fermentation; fermentation liquid of organic substances containing higher concentration thalli and enzymes and the like are obtained by filtering through an inorganic membrane; the solid fermentation process is adopted in the post-fermentation stage; agricultural byproducts such as wheat bran and the like are subjected to flash sterilization, used as fermentation raw materials, and subjected to solid fermentation in a ventilating fermentation tank; after the fermentation, the product of the feeding enzyme complex preparation is formed through the procedures of drying, crushing, granulating (for stabilizing the enzyme activity through the granulating process) and packaging. The process has the advantages of greatly reducing the treatment cost of the dextran waste liquid, and completely utilizing the dextran waste liquid to really realize the zero discharge of the production waste water of the dextran and improve the types and the activity of the enzymes in the fermentation products simultaneously.

The invention relates to a bio-fertilizer of an enzyme preparation. The fertilizer is characterized in that the raw materials (by weight) comprise 90-98 parts of fully fermented livestock and poultry dung or straw, turfy soil and silt, 2-10 parts of biologic enzyme complex including nitrogenase, phosphorous dissolving enzyme, potassium dissolving enzyme, plant growth promoting enzyme, heavy metal adsorptive enzyme and prophylactic enzyme. The fertilizer has the advantages of wide raw material sources, convenient treatment, simple production and management, and can guarantee the fertilizer efficiency.

The present invention belongs to a biologic enzyme complexes technical field, especially relates to a novel cellulase complexes and a preparation method thereof, including the following raw materials be weight percent: neutral cellulase 5%-30%, ammonium dibasic phosphate: 20%-40%, ammonium dihydrogen phosphate 5%-20%, fatty alcohol polyethenoxy ether 2%-15%, Na[2]SO[4]: 25%-50%, cornstarch :2%-15%, color paste: 0.1-0.2%. The invention replaces the traditional ellulase preparation, has a global performance, non dusting, a high catalytic activity, a rapid rocket, a very low back staining phenomenon, and is environmental protection, is used for indigo dye rinsing of bronchobuster clothing with a good rinsing effect, and the prepared bronchobuster clothing has a special reminiscence style.

The invention belongs to an enzyme complex extraction method in ganodermataceae and degreased silkworm pupa, which is characterized in that: mix the ganodermataceae and degreased silkworm pupa; add water; add food level cellulose and trypsinase and conduct the enzyme digestion under certain temperature; then filter, concentrate and dry the ingredients to get ganodermataceae polypeptide product which has the active ingredients of ganodermataceae, polypeptide and aminophenol and ensures double effects of ganodermataceae and polypeptide. Therefore, the invention has outstanding health-improving effect.

The invention discloses high-performance environment protective ostrich feed and a preparation method thereof. Earthworm meal, snail meal, housefly larva meal and yeast and other materials replace fish meal, meat and bone meal and other protein materials, peanut meal replaces soybean meal, side crops, such as cassava meal, replaces grain staple food, such as corn, wheat and the like, the polypeptide substance in the housefly larva meal, enzyme in the snail meal, enzyme complex in the multifunctional plasminogen activator, the flora of microbial probiotics, which restrain pathogenic bacteria and have other mechanism, replace chemical growth hormone and chemical drug antibiotic, and the raw materials are prepared into feed for adult ostrich and young ostrich. The feed is characterized in that the feed comprises the following raw materials according to part by weight: 30 to 60 parts of detoxicated cassava meal, 5 to 15 parts of earthworm meal, 5 to 20 parts of snail meal, 2 to 10 parts of housefly larva meal, 6 to 20 parts of yeast powder, 5 to 10 parts of peanut meal, 1 to 4 parts of garlic meal, 1 to 4 parts of mineral trace element, 2 to 5 parts of various amino acids, 0.5 to 2 parts of lecithin, 0.5 to 2 parts of various vitamins, 0.5 to 3 parts of multifunctional plasminogen activator, and 0.5 to 3 parts of microbial probiotics. The invention has the advantages that the quality of the feed is improved, the production cost is reduced, and the safety probability of the cultivated food is enhanced.