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Feeding complex enzyme preparation by fermentation of dextran waste liquor

A technology of compound enzyme and dextran for feed, applied in the directions of enzymes, enzymes, applications, etc., can solve the problems of high energy consumption and high cost of treatment, and achieve the effects of improving species and activity and reducing treatment costs.

Inactive Publication Date: 2009-03-11
李耀亭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of process technology that can solve the difficult problem of dextran production waste liquid treatment and produce feed compound enzyme at low cost at the same time, to make up for the deficiency that the existing dextran production waste liquid treatment consumes a lot of energy, and the cost is high, and reduces simultaneously Production cost of compound enzymes for feed

Method used

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  • Feeding complex enzyme preparation by fermentation of dextran waste liquor

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: 1000L of dextran waste liquid, adjusted to pH 5.0-6.0, pumped into a 1500L liquid fermenter, extinguished for 2 hours, inserted into Aspergillus niger at a volume ratio of 1:10-30, kept warm and ventilated for fermentation 7 days. Mix the Aspergillus niger fermented liquid with the steam-sterilized bran at a weight ratio of 1:1.3, put it in a ventilated fermentation tank for fermentation for 7-10 days, dry at 45°C, and pulverize to obtain the enzyme product. After measurement, acid protease activity is 4000U / g, cellulose (CMC) enzyme activity is 12000U / g, pectinase activity is 20000U / g, xylanase activity is 10000U / g, β-glucanase activity is 10000U / g, starch Enzyme activity 600U / g.

Embodiment 2

[0017] Embodiment 2: Dextran waste liquid 1000L, add KH 2 PO 4 , 1Kg; (NH 4 ) 2 SO 4 , 0.5Kg; CaCl 2 , 0.3Kg; MgSO 4 ·7H 2 O, 0.3Kg; FeSO 4 ·7H 2 O, 50g; MnSO 4 4H 2 O, 16g; ZnSO 4 ·7H 2 O, 14g; adjust the pH to 5.0-6.0, pump it into a 1500L liquid fermentation tank, and insert it into the Aspergillus niger strain at a volume ratio of 1:10-30 for 2 hours, heat preservation and aeration for 7 days. Mix the Aspergillus niger fermented liquid with the steam-sterilized bran at a weight ratio of 1:1.3, put it in a ventilated fermentation tank for fermentation for 7-10 days, dry at 45°C, and pulverize to obtain the enzyme product. After measurement, acid protease activity is 4000U / g, cellulose (CMC) enzyme activity is 20000U / g, pectinase activity is 50000U / g, xylanase activity is 20000U / g, β-glucanase activity is 20000U / g, starch Enzyme activity 400U / g.

Embodiment 3

[0018] Embodiment 3: Dextran waste liquid 1000L, add KH 2 PO 4 , 1Kg; (NH 4 ) 2 SO 4 , O.5Kg; CaCl 2 , O.3Kg; MgSO 4 ·7H 2 O, 0.3Kg; FeSO 4 ·7H 2 O, 50g; MnSO 4 4H 2 O, 16g; ZnSO 4 ·7H 2O, 14g; adjust the pH to 7.0-8.0, pump it into a 1500L liquid fermentation tank, and insert it into the Bacillus subtilis strain at a volume ratio of 1:10-30 for 2 hours, keep it warm at 38°C, and ferment with aeration for 3 days. After membrane filtration, the volume is concentrated to 120L, mixed with fermented Aspergillus niger solid fermentation material, the weight ratio is 1:1.3-1.5, mixed evenly, fermented at 23-26°C for 1-3 days, dried at 45°C, and crushed Enzyme products. After measurement, acid protease activity is 6500U / g, cellulose (CMC) enzyme activity is 22000U / g, pectinase activity is 60000U / g, xylanase activity is 25000U / g, β-glucanase activity is 28000U / g, starch The enzyme activity is 800U / g, and the viable count of Bacillus subtilis is 2.3×10 7 .

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Abstract

The invention discloses a process for fermenting and producing a feeding enzyme complex preparation by using dextran waste liquid, and the product thereof. The fermentation production process uses strains of aspergillus niger (05012) and strains of bacillus spore subtilis as enzyme-producing strains for production; the dextran production waste liquid is blended and then used as a raw material of liquid fermentation; fermentation liquid of organic substances containing higher concentration thalli and enzymes and the like are obtained by filtering through an inorganic membrane; the solid fermentation process is adopted in the post-fermentation stage; agricultural byproducts such as wheat bran and the like are subjected to flash sterilization, used as fermentation raw materials, and subjected to solid fermentation in a ventilating fermentation tank; after the fermentation, the product of the feeding enzyme complex preparation is formed through the procedures of drying, crushing, granulating (for stabilizing the enzyme activity through the granulating process) and packaging. The process has the advantages of greatly reducing the treatment cost of the dextran waste liquid, and completely utilizing the dextran waste liquid to really realize the zero discharge of the production waste water of the dextran and improve the types and the activity of the enzymes in the fermentation products simultaneously.

Description

technical field [0001] The invention relates to a process for producing a compound enzyme preparation for feed by fermenting dextran waste liquid and the compound enzyme preparation for feed produced by the process. Background technique [0002] Enzyme preparation is a high-efficiency biologically active substance, and it is also a "natural" and "green" feed additive. It can improve the production performance of livestock and poultry and reduce the pollution of excrement. At the same time, the development of feed resources provides the possibility to make the feed industry High efficiency, environmental protection and sustainable development become possible. [0003] The application of enzymes in the feed industry began in the late 1950s. In 1975, Kernin Company of the United States launched commercial feed enzyme preparations, which made the research on feed enzymes enter a relatively stable stage. Exogenous enzymes officially entered the stage of history as feed additives...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N9/42C12N9/26C12N9/24A23K1/16C12R1/685C12R1/125A23K10/18A23K20/189
Inventor 李耀亭
Owner 李耀亭
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