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Method for saccharifying cellulose raw material

A technology for cellulose and cellulose degrading bacteria, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of harsh requirements, polluted equipment, high energy consumption, etc., to avoid pollution and improve the degradation speed. , The effect of saving the cost of saccharification

Inactive Publication Date: 2010-10-06
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of large energy consumption, serious pollution and strict requirements on equipment in the traditional cellulose pretreatment process, so it improves the economic performance and is conducive to the development of my country's low-carbon economy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Dry the corn stalks, crush them into 40-mesh particles, take 2g of crushed raw materials, add 100ml GS-2 liquid medium (1.5g potassium dihydrogen phosphate per liter, 2.9g dipotassium hydrogen phosphate, 10g MOPS, 3g dihydrogen phosphate Sodium citrate water, 2.1g urea, 1.0g magnesium chloride hexahydrate, 150mg calcium chloride dihydrate, 1.25mg ferrous sulfate hexahydrate, 6g yeast extract and 1g cysteine ​​salt, and add 0.15ml 99% glacial acetic acid , pH=7.4), 0.2MPa nitrogen / hydrogen gas mixture (5% hydrogen) was passed through for 5 minutes to remove oxygen, and then sterilized at 121°C for 15 minutes;

[0040] 2) Prepare Clostridium thermocellum seed liquid, pick a single clone of Clostridium thermocellum, inoculate into 5ml GS-2 liquid medium (0.5% microcrystalline cellulose), and culture anaerobically at 60°C to OD 600 is 1.5;

[0041] 3) Inoculate 1ml of seed liquid into the medium containing corn stalks in step 1, and ferment in anaerobic sealed manner at...

Embodiment 2

[0044] 1) Prepare 100ml of GS-2 liquid medium containing 0.5% crystalline cellulose (1.5g potassium dihydrogen phosphate per liter, 2.9g dipotassium hydrogen phosphate, 10g MOPS, 3g sodium citrate dihydrate, 2.1g urea, 1.0g Magnesium chloride hexahydrate, 150mg calcium chloride dihydrate, 1.25mg ferrous sulfate hexahydrate, 6g yeast extract and 1g cysteine ​​salt, pH=7.4), feed 0.2MPa nitrogen / hydrogen gas mixture (in which the hydrogen content is 5 %) deoxygenation for 5 minutes, 121 ° C sterilization for 15 minutes;

[0045] 2) Preparation of Clostridium thermocellum seed liquid: Pick a single clone of Clostridium thermocellum, inoculate it into 5ml GS-2 liquid medium (0.5% microcrystalline cellulose), and culture it anaerobically at 60°C for 36 hours to OD 600 is 1.5;

[0046] 3) Inoculate 1ml of seed solution into the culture medium of step 1, and ferment in anaerobic seal at 60°C for 72 hours, and then measure the filter paper enzyme activity of cellulosomes in the ferme...

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Abstract

The invention discloses a method for saccharifying a cellulose raw material. A cellulosome secreted by high-temperature thermoanaerobion can effectively reduce degrade crystalline cellulose with a complex structure. By adding low concentration acetate into a culture medium and enzyme reaction buffer solution and filling low concentration hydrogen in a fermentor thereof, the method greatly improves the enzyme reaction speed and sugar accumulation. The method can make the cellulose material which is not subjected to physical or chemical pretreatment directly fermented or subjected to enzyme treatment, and efficiently hydrolyze the cellulose material into reducing sugar.

Description

technical field [0001] The invention relates to the field of bio-energy industry, in particular to a method for saccharifying cellulose raw materials by using high-temperature bacteria and their unique cellulase system. Background technique [0002] With the development of the world economy, greenhouse gas emissions, global warming, and epidemic diseases are increasingly threatening the survival and safety of human beings. How to properly handle the relationship between human beings and the natural environment and maintain a good ecological balance has become the biggest challenge facing governments in the future. The only solution is that we need to gradually get rid of our dependence on fossil energy and look for non-polluting alternative energy. Cellulose is the most abundant renewable resource on earth, and terrestrial plants can produce about 50 billion tons of cellulose every year. In addition, cellulose resources are the most important biomass resources, which accou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12P19/14C12R1/145C12R1/01
Inventor 许成钢宋厚辉李玉东徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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