A method for tissue culture and rapid propagation of Xianwang jujube

Abstract

The invention belongs to the technical field of plant tissue culture and in particular relates to a tissue culture rapid propagation method of Xianwang jujubes. The method comprises the steps of explant establishment, initiation culture, subculture, rooting culture, hardening and transplanting. The method has the beneficial effects that researches are conducted aiming at the variety of Xianwang jujubes and the most appropriate culture medium composition is adopted at different stages, so the propagation coefficient is high and the rooting rate is high; the method is simple and convenient to operate, is low in cost and can achieve the effect of effectively and conveniently obtaining plenty of Xianwang jujube tissue culture seedlings in a short time; the obtained tissue culture seedlings have high survival rates and can maintain good genetic characters of superior trees; plenty of uniform regeneration plants with stable genetic characters can be obtained by adopting the tissue culture rapid propagation method of Xianwang jujubes, have stable test results and good repeatability, can be applied to large-scale commercial seedling raising and are suitable for being planted in large-area regions in the northern mountain regions in Guangxi, especially in the mountain regions with karst landforms.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Xianwang jujube. Background technique [0002] "Xianwang Zao" is an excellent strain selected from Jinsi Xiaozao by the Forestry Bureau of Xianxian County, Hebei Province, and was approved by the Hebei Provincial Forestry Appraisal Committee in 2005. Because it is mainly distributed in the area around the Zhenxian King's Mausoleum in Hejie, Xianxian County, Hebei, it is named "Xianwang Zao". Xianwang jujube has a strong structure, strong branches, natural semi-circular crown, and open tree posture. The jujube head is brownish red, and the jujube hangs in the middle. Leaves are ovate or broadly lanceolate, with serrated margins. The amount of flowers is medium and large, with 5-8 flowers per inflorescence, and the flower diameter is about 6 mm. The fruit is oblong, with an average fruit weight of 9 g...

AI Technical Summary

Problems solved by technology

So far, people have made some achievements in the study of jujube tissue culture, and the technology of using tissue culture to propagate fine varieties of jujube has been gradually applied to production. However, the tissue culture of jujube is difficult and expensive, and different varieties There are large differences in the propagation conditions among different varieties of jujube trees, and the tissue culture methods of different varieties of jujube trees are also different.

[0005] At present, in the breeding of Xianwang jujube, due to the use of traditional methods such as cuttings and grafting for breeding, there are problems such as very low breeding rate, slow growth rate, and far from meeting the large demand of the market; therefore, the development of a The tissue culture and rapid propagation method for Xianwang jujube can promote the rapid propagation of excellent genetic materials, realize the mass reproduction and rapid promotion of Xianwang jujube seedlings, and is an effective method to solve market demand and other problems

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