363 results about "Simple sample" patented technology

The invention belongs to the technical field of image processing and computer vision, and relates to a novel biomedical image automatic segmentation method based on a U-net network structure, including dividing a biomedical data set into a training set and a test set, and normalizing the test set and augmented test set; inputting the images of the training set into the improved U-net network model, and generating a classification probability map by output image passing through a softmax layer; calculating the error between classification probability diagram and gold standard by a centralized loss function, and obtaining the weight parameters of network model by a gradient backpropagation method; entering the images in the test set into the improved U-net network model, and outputting the image to generate a classification probability map through the softmax layer; according to the class probability in the classification probability graph, obtaining the segmentation result graph of theimage. The invention solves the problems that simple samples in the image segmentation process contribute too much to the loss function to learn difficult samples well.

The invention discloses a method for testing mechanical property and reliability of a thin film material of a micro-electromechanical system (MEMS) and a testing device thereof. A thin film testing structure with a suspended membrane is fixed on a sample platform with a gas passage and a differential pressure is imposed on one side of the suspended membrane to cause the other side of the suspended membrane to be deformed and heave; the deflection at the center of the membrane under the impressed pressure is measured, namely the maximum deflection of the membrane and the pressure is gradually increased to cause the suspended membrane to fracture; such parameters related to the mechanical property and reliability of the tested thin film material as plane strain modulus, Young modulus, Poisson ratio, residual stress, rupture strength and the like are extracted and obtained with the measurement of a pressure-deflection change curve and in accordance with a theory calculating model. The invention adopts a non-contact optical method, utilizes a data acquisition and control unit to automatically implement the measurement of the deflection and is characterized by simple sample preparation and testing, high measurement accuracy, convenient and flexible operation and high testing efficiency.

The invention discloses a method and device for detecting somatic cell SNP. The method comprises the steps of S1, comparing DNA sequencing data of somatic cells with reference genome data to obtain data of all SNP loci; S2, screening out SNP loci which occur on splicing loci and SNP loci which occur on exons and cause nonsynonymous mutation of amino acid to obtain a first SNP locus set; S3, removing SNP loci with the thousand-people mutation frequency higher than 5% in the first SNP locus set to obtain a second SNP locus set; S4, screening out SNP loci which are resources of somatic cell mutation from the second SNP locus set to obtain a third SNP locus set; S5, screening out SNP loci with the frequency of supporting sequences lower than 75% from the third SNP locus set to obtain SNP loci containing somatic cell SNP. By means of the method, somatic cell mutation can be detected through simple samples, and therefore the cost is lowered.

The invention discloses a method for constructing a face recognition model based on a ParaSoftMax loss function, which comprises the following steps: selecting a basic convolution neural network modelaccording to an application environment of a task; acquiring a face image marked with human face identity information in a specified number as a training data set; the decision edge parameters are obtained according to the difference of the class center angles between the difficult sample eigenvectors and the simple sample eigenvectors and the class center angles in the basic convolution neural network model. Obtaining a ParaSoftMax loss function according to the decision edge parameter; setting the loss function at the last layer of the basic convolution neural network model to form a face recognition model based on the loss function; input the training data set to the face recognition model, minimizing the loss function iterative training model parameters, and obtaining the optimal facerecognition model. Thus, the face recognition model of the present application can improve the accuracy of face recognition.

The invention discloses a Kalman filtering and data driving fusion battery SOC estimation method belonging to the battery SOC estimation method technology field. The invention provides a varied variance Kalman filter least square support vector machine (VVKF LSSVM) fusion method. Based on two equations of a KF, a noise variance, which is adapted to a current system state to the greatest extent, is set during every iteration, and a problem of declined precision caused by Kalman filtering noise variance initial value relied on artificial experience setting is solved. A least square support vector machine (LS SVM) is selected as the measurement equation of the KF, and by starting from a data angle, the SOC estimation method suitable for various batteries is completed by establishing a simple sample library, and the estimation precision is improved by dynamic modeling. A part of data in an NASA lithium battery data set and a CACLE lithium battery data set is used for experimenting to prove the superiority of the VVKF by comparing with the KF, and the validity of the whole method on the lithium battery SOC estimation.

The invention discloses a raman reinforced substrate material, preparation and application methods thereof. A preparation method comprises the following steps: firstly, preparing a TiO2 nanometer tube array; depositing graphene on the TiO2 nanometer tube array; and lastly, dropwise adding nanometer silver sol to the surface of a substrate, thereby forming a compound material after the nanometer silver sol is dried. The convenient and quick detection for various harmful substances in an environmental sample, especially for benzo (a) pyrene, can be realized by using a raman reinforced substrate. A new method for detecting the benzo (a) pyrene is provided according to an application method of the raman reinforced substrate material. Compared with a traditional method, a surface reinforced raman detection method using the raman reinforced substrate material has the advantages of short detection time and simple sample treatment.

The invention discloses a method of detecting flood disaster changes through object-level high-resolution SAR (synthetic aperture radar) images and aims to solve the problem that a false target possibly existing in an SAR image and similar to a water region in geometrical characteristics and spectral characteristics causes great 'pseudo-changes' and causes difficulty and disturbances to flood disaster change detection. Each time-phase image is subjected to contourlet transform; image noise is suppressed at the premise of keeping image edge characteristics, an optimal decomposition scale is selected through simple sample training, and the position of a mark point in a possible area of a water body is quickly acquired through block histogram statistics, on the optimal decomposition scale. Contour information of the water body is acquired through a mark-point-based watershed segmentation and region merging strategy, and interference of the false target is further eliminated through discrimination rules based on multiple features. Finally, water contours extracted from multiple time-phase images are directly compared according to registration results, and a region having water body changes is obtained.

The invention discloses a method and a special quantum dot fluorescent immunoassay kit for detecting quinolone compounds. The quantum dot fluorescent immunoassay kit for detecting the quinolone compounds provided by the invention comprises specific antibodies, coating antigens and standard solution of the quinolone compounds, wherein the coating antigens are conjugates of hapten and carrier proteins of the quinolone compounds. Experiments prove that the kit of the invention has the characteristics of simple sample pretreatment process, simple and convenient operation, low cost, high specificity, high sensitivity, high accuracy, on-site monitoring, suitability for screening of a large number of samples, and the like.

The invention discloses a method for rapidly detecting the content of aflatoxin. The method comprises the following steps of: after smashing a sample to be detected, putting the sample powder into a beaker, and adding an extractant for shaking or performing ultrasonic extraction; filtering, performing water bath evaporation on a filtrate, and metering the volume to obtain a test sample; respectively pointwise adding the test sample and an aflatoxin B1 standard substance which are commensurate on a thin-layer silica gel plate, pointwise adding derivative reagents into sampling points, and respectively developing by developers; and comparing the fluorescence intensity of the test sample and the aflatoxin B1 standard substance under an ultraviolet lamp to judge whether the content of the aflatoxin in the test sample exceeds the standard. The method for rapidly detecting the content of the aflatoxin has the advantages of small solvent amount, simple sample treatment and short measurement time; the method does not need a professional, only needs small apparatus, and is particularly suitable for rapid field detection; and the method effectively avoids the harm and the pollution of trifluoroacetic acid to operators and the environment, improves the method sensitivity, and has the lowest detectable amount of 0.00032 mu g and the lowest detection limit of 3 mu g / kg.

The invention discloses a method for detecting tetracycline hydrochloride by using terahertz time-domain spectroscopy, which comprises the following steps: preparing tetracycline hydrochloride tablet samples with different mass percents; acquiring terahertz time-domain spectra of the tetracycline hydrochloride tablet samples; performing Fourier transformation treatment on the terahertz time-domain spectra of the tablet samples, and obtaining absorption coefficients and refractive indices of the tablet samples through a formula; establishing a correction model according to the mass percent of each tablet sample and the corresponding absorption spectrum; and verifying and evaluating the correction model. According to the invention, the qualitative and quantitative detection of tetracycline hydrochloride can be realized; and the method has the advantages of simple sample pretreatment, easy operation, favorable repetitiveness, relatively accurate test result and the like. Thus, the method is of far reaching importance for detecting antibiotic residue in foods by using terahertz time-domain spectroscopy.

The invention discloses a nondestructive testing method for absorption coefficient and refractive index of a pesticide. The method comprises the following steps of: testing nitrogen and a pesticide test sample by using a transmission-type terahertz time domain spectroscopy (THz-TDS) to obtain time domain spectrums of the nitrogen and the pesticide test sample respectively; and performing data processing to obtain refractive index spectrum and absorption spectrum of the pesticide test sample at the THz wave band of between 0 and 2.5THz, wherein the pesticide comprises chlorpyriphos methyl, baygon and fenvalerate. By the method, rapid nondestructive testing of the pesticide can be realized; and the method has the advantages of simple sample preprocessing, easiness of operation, high repeatability, simple data processing, relatively accurate testing result and the like. The method has great significance to detection of pesticide residue on fruits and vegetables by using the terahertz time domain technology based on fingerprint spectrums of the three pesticides obtained by the method.

The invention discloses a method for rapid identification of Gerbera jamesonii ploidy by using flow cytometry. The method comprises steps of: preparing a unicell fluid suspension by using an extraction cracking buffer and a dyeing buffer; determining DNA fluorescence intensity of a sample by using a CyFlow Cube type of flow cytometer produced by German partec company and carrying out ploidy analysis; and comparing the abscissa positions of the sample to be measured and the contrast DNA content fluorescence intensity separation peak to identify the ploidy of the sample to be measured. A flow cytometer ploidy identification result is consistent with root tip chromosome counting ploidy. According to the method, ploidy of batch of Gerbera jamesonii macrospore regeneration plant can be screened out rapidly, and efficiency of DH colony construction and heredity atlas drafting is increased substantially, so as to accelerate a breeding process. The invention has advantages of simple sample preparation, rapid detection and stable high detection rate.

The invention puts forward an embedded attitude learning method capable of carrying out self supervision on the basis of a video time-space relationship. The average value of two auxiliary tasks is used for learning the time-space relationship in a video: one time sequence task learns whether two specific figure images are near or not on an aspect of time, and one spatial arrangement task learns ahuman body appearance model from space to enhance a capability of separating a gesture from a background. On the basis of the learning and mining repeated gesture of a course, training is carried outfrom a simple sample, then, the simple sample is iteratively expanded to a harder sample, and meanwhile, inactive video parts are eliminated. Time-space embedding successfully learns the representative characteristics of human gestures in a self-supervision way. By use of the method, a time-space relationship training video is used for carrying out gesture embedding self-supervision learning, manual annotation is not required, cost is lowered, gesture embedding can capture the visual characteristics of the human gestures, and human gesture estimation and retrieval efficiency can be improved.

The invention discloses a method for online detecting the near infrared spectrum of active ingredients of compound eucommia bark capsules. The active ingredients of the compound eucommia bark capsules mainly comprise pinoresinol diglucoside, baicalin, rhynchophylline, isorhynchophylline, and the like. The method comprises the steps of collecting the near infrared absorption spectrum of the compound eucommia bark capsules, associating active ingredients content data obtained by an HPLC (High Performance Liquid Chromatography) method with the near infrared spectrum of a sample, establishing a correction model by a partial least squares cofinal method and realizing quick online detection of the active ingredients of the capsules. Research results show that the near infrared spectrum analysis method can effectively detect the active ingredients of the compound eucommia bark capsules, has simple sample, quick detection and multiple components detection at the same time, can be used for real-time analysis and online quality control during pharmaceutical processing of the traditional Chinese medicine for controlling the quality of medicines in all dimensions, enhances production automation and informatization level of traditional Chinese medicine and promotes the development of the pharmaceutical industry.

The invention relates to a poly-pyrrole modified electrode doped with p-toluene sulphonic acid and provides a method using the modified electrode for rapidly detecting the content of vitamin E. the method comprises: 1) using experimental conditions which are the same with the detecting conditions of a sampler for drawing a standard curve of the content of vitamin E and oxidation peak current; 2) dissolving the sample in the mixed solvent of ethanol-1 and 2 dichloroethane and regulating the pH value to be 2-5 to obtain the sample solution; 3) arranging the modified electrode in the sample solution, detecting the oxidation peak current through a differential pulse voltammetry and calculating the concentration of vitamin E in the sample. The method can be used for rapidly detecting the content of vitamin E in vegetable oil and has the advantages of simple sample preparation, convenience, fast speed, strong anti-interference ability and accurate detecting result which is identical with the detecting result of a liquid chromatography.

The invention provides a method for detecting impurity elements in tungsten carbide, and concretely relates to the method for rapidly detecting tungsten carbide as well as cobalt, nickel, iron, titanium, aluminum, manganese, magnesium, vanadium, chromium, copper, and molybdenum in casting of tungsten carbide by using an inductively coupled plasma-atomic emission spectrometry. A sample is digested in an electrothermal digestion instrument by employing hydrofluoric acid and nitric acid, under condition that a tungsten matrix is not separated, the high purity tungsten matrix is used for preparing a standard work curve, spectrum interference of the tungsten matrix to a to-be-measured element is solved, optimum wavelength of each element is selected, background correction is carried out, and tungsten carbide as well as cobalt, nickel, iron, titanium, aluminum, manganese, magnesium, vanadium, chromium, copper, and molybdenum in casting of tungsten carbide are rapidly detected by using the inductively coupled plasma-atomic emission spectrometry. The detection method has the advantages of simple operation, short detection period, simple sample processing, and wide detection scope, and is suitable for batch production analysis.

The invention provides a method for detecting a crab freshness grade based on machine olfaction. The method aims at collecting smell information and detecting TVB-N of crab samples at different storage periods; pre-treating obtained smell information, wherein the step of pre-treating comprises average-filtering, baseline treatment, and removal of abnormal data; then selecting characteristics capable of effectively representing the smell information; reducing dimensions by adopting non-linear dimension-reducing Laplacian Eigenmap according to a multi-dimension characteristic; establishing a prediction model of the crab freshness grade since a visualized analysis result corresponds to a TVB-N detection result; taking classified information of the visualized result as outputting of the prediction model, and taking the effective characteristics as inputting; and testing the trained model by using a unknown sample to obtain a result of a final crab freshness grade. According to the invention, novel machine olfaction technology is adopted to detect the crab freshness grade. The method has advantages of simple sample treatment and fast detection speed, and has no damage on crabs, and has huge application value in aquatic product markets and people's daily lives.

The IMS apparatus and methods described in this invention involve setting the ion detector at the highest potential of the drift tube and setting the ionization source at ground or near ground potential. The methods allow significantly simple sample introduction without the limitation of the high potential (voltage) concern of the front end sample delivery. The invention also describes bringing samples directly into the ion mobility drift tube. The invention further describes using single syringe for sample introduction via an electrospray ionization method.

The invention belongs to the technical field of microelectronics, and in particular discloses a 3 omega thermal conductivity measurement method suitable for a metal film material. In the method, a group of frequency-related heat flow ratios is obtained through an experimental fitting means by utilizing a 3 omega test structure and frequency-related thermal response characteristic based on an improved analytical model. The thermal resistance of an experimental material is deduced from the ratios, and a thermal conductivity value of a tested sample is finally obtained. The thermal conductivity measurement method of the invention can provide rapid and accurate thermal conductivity information of a metal film, so that the application range of 3 omega electrical measurement technology is greatly extended. The 3 omega thermal conductivity measurement method is suitable for measuring nanoscale films, has a simple sample structure, and avoids a complex process structure in the conventional electrical measurement method; therefore, the 3 omega thermal conductivity measurement method can be taken as a rapid characterization means for a thermal parameter of the metal film material, and has application prospect in the microelectronic industrial field.

The invention discloses a high performance liquid chromatographic analysis method for pyromellitic dianhydride. The method comprises the following steps: (1) adopting chromatographic conditions, (2) preparing a mobile phase, (3) preparing a PMDA standard solution, (4) building a standard curve, (5) preparing a PMDA sample solution, and (6) carrying out quantitative analysis by using an external standard percentage method. The invention provides the method for quantitatively analyzing pyromellitic dianhydride, which fills the technological gaps of corresponding fields. The method has the advantages of simple sample treatment and analytical procedure process, good peak shape, high analysis result accuracy, good reproducibility and the like and has an important practical significance in the fields of quality analysis and quality control of PMDA.

The invention relates to the technical field of cigarette product detection, and provides a sample pretreatment method in occurrence form detection analysis on chromium in tipping paper for cigarette. According to the sample pretreatment method, by summarizing a targeted solving technical scheme of a sample pretreatment technique scheme of the tipping paper for cigarette, a rapid form analyzing method and a pretreatment method of HPLC-ICP-MS (High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry) are established aiming at inorganic trivalent chromium (Cr3+) and hexavalent chromium (Cr6+); aiming at the organic chromium, organic solvent extraction is established; and the rapid and simple sample pretreatment method of the organic chromium is carried out based on low pressure sealed decomposition of a PFA (Polyfluoroalkoxy) self-sealed tank, so that the organic / inorganic chromium can be completely decomposed, and the ultrasonic auxiliary rapid extraction on the organic chromium is realized by using ethyl acetate as the extracting agent. By utilizing the method, the occurrence form and content level of the chromium in the tripping paper can be clearly analyzed and detected, and whether the chromium in the tipping paper can enter human body through spittle contact or not can be accurately judged.

The invention discloses a fluorescent probe for detecting various metal ions in water, and a preparation method and application thereof. The probe is made up of symmetrical tetramethyl cucurbit [6] uril and trans-4-4(Dimethylamino)styryl-1-methylpyridine iodine. The preparation method comprises the following steps: dissolving tetramethyl cucurbit [6] uril in water to obtain a solution A; dissolving trans-4-4(Dimethylamino)styryl-1-methylpyridine iodine in water to obtain a solution B; and mixing the solution A and the solution B, and reacting at normal temperature to obtain the probe. The probe is mainly used to detect caesium, strontium, barium, iron, chromium, mercury, europium,thulium and / or ytterbium ions in water. The probe disclosed by the invention can be used to detect various ionsof caesium, strontium, barium, iron, chromium, mercury, europium,thulium and / or ytterbium in water, and has the characteristics of high sensitivity, low detection cost, simple sample processing, convenient operation, rapid determination and real-time detection.

The invention discloses a honey adulteration detection method based on HPLC-ELSD and a partial least squares discriminant analysis method, and belongs to the technical field of food detection. The method comprises simple sample pretreatment, wherein the simple sample pretreatment comprises the steps of adopting a hydrophilic interaction chromatography separation-evaporation light scattering detector (HILIC-ELSD) method to analyze oligosaccharide and isomer components of oligosaccharide in authentic honey and a honey sample adulterated with fructose corn syrup or malt syrup, and utilizing difference of oligosaccharide component fingerprint spectrums of the authentic honey and the adulterated honey to conduct data processing through the partial least squares discriminant analysis method, so that adulteration identification is further achieved. The honey adulteration detection method based on the HPLC-ELSD and the partial least squares discriminant analysis method can effectively identify adulterated honey with C4 botanical syrup or C3 botanical syrup, the adopted analysis instrument is popular, and the method is convenient for popularization and application.

The invention discloses a detection method for high throughput liquid chromatogram tandem mass spectrum. The method comprises the following steps: (S1) preparing a sample; (S2) performing derivatization reaction; (S3) pretreating the sample; (S4) separating the liquid chromatogram; and (S5) detecting the tandem mass spectrum. Besides, the invention also discloses a method for detecting four catecholamine metabolites through a high throughput liquid chromatogram tandem mass spectrometry. The detection method provided by the invention is fit for the detection for the catecholamine metabolites in human plasma and has the advantages of simple sample pretreatment, capability of simultaneously detecting four catecholamine metabolites, excellent detection specificity, short time in the whole detection process and high throughput.

The invention discloses an approximate optimization method based on particle swarm optimization and Kriging model, which belongs to the technical field of engineering optimization design. A method forrealize that invention comprises the follow steps of: using particle swarm evolution to guide simple sample points to aggregate to a global optimal solution; adopting fuzzy c- Mean clustering methodis used to cluster simple sample points to identify the region of interest, so as to fully mine the design space information on the basis of balancing local search and global exploration. By adding high-precision sample points in the region of interest sequence and updating the Kriging model constantly, the optimization converges to the global optimal solution quickly. The invention can effectively improve the global exploring ability and the local searching ability of the optimization method, reduce the calculation cost in the optimization process of the complex engineering system, avoid missing the global optimal solution as far as possible, and improve the reliability of the design result. The invention is suitable for application in the field of complex engineering system design optimization including a high-precision analysis model, and can solve corresponding related engineering problems.

The invention discloses a high-throughput liquid chromatography tandem mass spectrometry detection method with high-throughput liquid chromatography tandem mass spectrometry, wherein the method includes the steps of S1 sample preparation, S2 sample pretreatment, S3 liquid chromatography separation, S4 tandem mass spectrometry detection and the like. In addition, the invention also discloses the method for detecting 15 kinds of bile acids by the high-throughput liquid chromatography tandem mass spectrometry method. The method provides the high-throughput liquid chromatography tandem mass spectrometry detection method, wherein the detection method is suitable for detection of the bile acids in human serum, has simple sample pretreatment, can simultaneously detect 15 kinds of bile acids, andhas the advantages of good detection specificity, short whole detection process time and high throughput.

The invention relates to a building material detection technology and provides a method and a system for non-contact electrical resistivity-based evaluation of permeability of a cement-based material. The system comprises two semicircular groove molds with covers. The semicircular groove molds pass through two coils of a non-contact electrical resistivity tester and then are butt-jointed, and the butt-joint is sealed tightly by glass cement so that solution leakage is prevented. Two test samples of which a part of circumference is circular compose a group. The test samples are put into the semicircular groove molds. Vaseline is coated between inner walls of the semicircular groove molds and the surfaces of the test samples close to test sample ends and is used for preventing direct permeation of the solution. A common salt solution is poured into the semicircular groove molds and the level height of the common salt solution in the semicircular groove molds is equal to that of the test samples. The method and the system can overcome defects of the prior art, and realize objective, accurate and reasonable evaluation of permeability of a cement-based material. The method has the advantages of simple sample preparation processes, simple operation, short test period and low test cost.

The invention relates to a homocysteine Hcy kit based on an aptamer fluorescence probe; at the same time, the invention also relates to a method for determination of the concentration of homocysteine Hcy, and a determination reagent composition and components, and belongs to the technical field of medical examination and determination. The kit comprises the main components: an erythrocyte lysate, a phosphate buffer solution PBS, a homocysteine Hcy standard substance, and the homocysteine Hcy aptamer fluorescent probe; through blood sample cracking and mixed egg fertility treatment, and with combination of a fluorescence spectrophotometer for detection, the value of the concentration of the homocysteine Hcy is measured and calculated. The kit has the advantages of simple sample treatment, simple and convenient operation, short detection time, strong detection specificity, high sensitivity, high repeatability of detection results and the like.

This invention describes a general magnetic binding assay method to detect the presence and quantity of analyte in a sample. The method uses magnetic particles for separating and concentrating analytes of interest from complex samples and use time-resolved up-converting fluorescence detection technique to provide highly sensitive detection without using expensive optical components such as band-pass filters. The method uses pulsed long wavelength light for excitation and time-delayed luminescence detection, resulting in little interferences from sample matrices. Furthermore, the usage of long wavelength excitation light requires simpler sample preparation and clean-up such as removal of red blood cells, which otherwise will significantly interfere with excitation efficiency of the fluorescence probes.