36 results about "Pinoresinol" patented technology

The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

Disclosed is an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction for transferring a hydroxy group into a lignan, preferably an enzyme capable of catalyzing the hydroxylation reaction from piperitol into 9-hydroxypiperitol or from pinoresinol into 9-hydroxypinoresinol. Specifically disclosed are: a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the polypeptide; a vector or transformant carrying the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity by using the transformant; and others. The transformant having the polynucleotide introduced therein in an expressible manner is useful for the hydroxylation of a lignan or the production of a product by using lignan hydroxide in the fields of foods and various industries.

The invention discloses a method for measuring the content of pinoresinol diglucoside in eucommia ulmoides medicinal materials. The method comprises the following steps: Step 1): preparing a referencesolution of pinoresinol diglucoside by using a methanol solution as a solvent; Step 2): processing eucommia ulmoides medicinal materials into floccules to obtain a sample to be detected; Step 3): sequentially extracting the sample to be detected by chloroform and methanol, and dissolving the sample with methanol to obtain a test solution; Step 4): measuring the content of pinoresinol diglucosidein the reference solution and the test solution by high performance liquid chromatography; the chromatographic detection conditions are as follows: octadecylsilane chemically bonded silica is used asa filler; the volume ratio of a mobile phase is 25:75 methanol-water solution; the detection wavelength is 277 nm; the sample size is 10 uL; and the technical scheme adopted by the invention solves the problems that the spectrogram obtained by the eucommia ulmoides detection method has poor chromatographic peak performance and more miscellaneous peaks when the eucommia ulmoides content is determined in the prior art.

The invention provides a throat-smoothing medicine with effects of relieving throat discomfort, soothing throat and relieving stuffy nose. The throat-smoothing medicine comprises the following raw materials in parts by weight: 8 to 12 parts of glucose, 6 to 7 parts of sugarcane, 13 to 37 parts of water, 5 to 8 parts of pigment, 15 to 20 parts of natural mint, 15 to 18 parts of eucalyptol, 8 to 12 parts of thymol and 7 to 10 parts of pinoresinol. Compared with the prior art, the throat-smoothing medicine has the beneficial effects that a plurality of effects of clearing and benefiting throat, clearing heat and detoxicating, eliminating swelling and relieving pain and the like are realized; the therapeutical effect of treating voice hoarseness, acute laryngopharyngitis and the like is good; the main ingredients are herbaceous plant extracts, so that no side effect is generated after use, and the throat-smoothing medicine is suitable for various populations.

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

Aiming at the phenomena that pinoresinol in the extraction process is easy to decompose to severely affect production of the pinoresinol on a large scale, the method provided by the invention separates and purifies pinoresinol and chlorogenic acid gradually by adopting ethanol extraction, rising film concentration, column chromatography and chromatographic separation, so that the requirements on production of the pinoresinol and chlorogenic acid on a large scale are realized, thereby effectively improving the yield of pinoresinol in eucommia leaves and meanwhile purifying high content chlorogenic acid.

The invention relates to a method for detecting whole eucommia capsules, belonging to the technical field of medicine. The present invention adopts the same chromatographic condition to simultaneously measure the content of four kinds of lignan compounds such as pinoresinol diglucoside, pinoresin monoglucoside, syringin and pinoresin in the whole Eucommia capsule, and the operation is simple and fast, and the determination result is accurate and heavy. It has good presentability and can better reflect the product quality of Eucommia ulmoides capsules.

The invention relates to the field of separation and detection of substances, and provides a method for synchronous detection of coniferyl alcohol and pinoresinol. First, high performance liquid chromatography is used to measure the absorbance of coniferyl alcohol and pinoresinol in a sample to be tested, wherein the detection wavelength is 280nm; The high-performance liquid chromatography adopts gradient elution, and the eluent in the gradient elution includes methanol and water; then according to the standard curve of coniferyl alcohol and the absorbance of coniferyl alcohol in the test sample, the coniferyl alcohol in the test sample is obtained. The concentration of alcohol; According to the standard curve of pinoresinol and the absorbance of pinoresinol in the test sample, obtain the concentration of pinoresinol in the test sample. The method provided by the invention can effectively separate coniferyl alcohol and pinoresinol in the sample to be tested, and detect the corresponding concentrations of coniferyl alcohol and pinoresinol respectively.

The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

The invention relates to a method for enzymatic synthesis of pinoresinol active substances, which includes the steps: mixing water or phosphate buffer solution with water-soluble organic solvent into mixed solution; adding pinoresinol substrate into the mixed solution; adding Rhus Laccase to react at the temperature of 20-45 DEG C for 48-96 hours; after reaction, adding a proper amount of methanol and sodium tartrate into reaction product, subjecting the reaction product to ethyl acetate extraction, anhydrous sodium sulfate drying and concentration, and performing methanol recrystallization to obtain crystal or performing silica-gel column chromatography, concentration and purification to obtain chromatographic product; and subjecting the crystal or chromatographic product to nitrogen blowing or freeze-drying to obtain pinoresinol active substances. The method has moderate conditions, reaction is environment-friendly, simple, quick and low-cost, and the method has broad application prospect in the fields of preparing similar biological medicinal products, active complex compounds, and the like.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a eucommia lignanoid preparation and a preparation method of the eucommia lignanoid preparation. The eucommia lignanoid preparation comprises the following raw materials in percentage by weight: 5%-20% of pinoresinol diglucoside, 5%-15% of syringaresinol diglucoside, 1%-10% of pinoresinol A diglucoside, 5%-15% of olivil biglucoside, and 1%-5% of naringenin B, wherein a eucommia lignanoid extract preferably contains 10%-15% of pinoresinol diglucoside, 8%-12% of syringaresinol diglucoside, 5%-8% of pinoresinol A diglucoside, 8%-12% of olivil biglucoside and 2%-4% of naringenin B.

The invention relates to the field of substance separation detection and provides a synchronous detection method of coniferyl alcohol and pinoresinol. Absorbance values of coniferyl alcohol and pinoresinol in a to-be-detected sample are measured by using high performance liquid chromatography, wherein the detection wavelength is 280 nm and the high-performance liquid chromatography employs gradient elution and the eluent in the gradient elution includes methanol and water; according to a standard curve of coniferyl alcohol and the absorbance of the coniferyl alcohol in the coniferyl alcohol, the concentration of the coniferyl alcohol in the to-be-detected sample is obtained; and on the basis of the standard curve of pinoresinol and the absorbance of pinoresinol in the to-be-detected sample, the concentration of the pinoresinol in the to-be-detected sample is obtained. Therefore, the coniferyl alcohol and pinoresinol in the to-be-detected sample can be separated effectively; and the corresponding concentrations of the coniferyl alcohol and pinoresinol can be detected respectively.

The invention discloses a method for preparing pinoresinol from salted turnips, which comprises the following steps: 1, the preparation of dry salted-turnip powder: slicing salted turnips, drying at a temperature of 50 DEG C, crushing, sieving by using a 20-mesh sieve so as to obtain dry salted-turnip powder, putting the dry salted-turnip powder into a sealed bag, and putting the sealed bag into a dryer for standby application; 2, the preparation of a salted-turnip crude extract; 3, the preliminary separation of the salted-turnip crude extract; 4, the separation and purification of a component Y1; and 5, the separation and purification of components 1-2: after a moderate amount of alcohol is added into the components 1-2 for dissolving, enabling the obtained object to pass through a reverse-phase silica gel column to carry out separation, eluting by using 50% CH3OH, and selecting a fourth flow component and separating by using a normal phase silica gel; eluting by using petroleum ether, collecting flow components of 97th to 116th pipes, merging the flow components, concentrating the obtained object, and removing a solvent by evaporating, so that pinoresinol is obtained. According to the invention, pinoresinol is separated from salted turnips for the first time, and as a main antifungal ingredient of the salted turnips, the pinoresinol is of great significance for the secondary development of salted turnips.

The invention belongs to analysis and detection of drugs, and particularly relates to a method for simultaneously detecting various effective components in compound lonicera granules with high performance liquid chromatography. The method is used for simultaneously detecting neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, forsythiaside A, forsythin, pinoresinol, baicalin and wogonin in the compound lonicera granules with the high performance liquid chromatography and comprises steps as follows: preparation of a reference substance solution, preparation of a solution of a testing product, determination, result calculation and the like. The method effectively solves the problems that quality evaluation of the compound lonicera granules is mainly about single-component content determination and the like in the prior art, and has the advantages of comprehensively characterizing active pharmaceutical ingredients of the compound lonicera granules and being high in repeatability and good in precision and the like.