36 results about "Pinoresinol" patented technology

The invention discloses an effective part extract of valeriana amurensis P.Smirn. and a quality control method and medical use thereof. The content of total lignanoid substances in the extract accounts for over 50 percent of the total weight of the extract, wherein (+) pinoresinol-4,4'-O-beta-D-diglucopyranoside and (+) pinoresinol-8-O-beta-D-glucopyranoside are the main active components in the effective part extract, and the sum of the contents of the two active components accounts for more than 13 percent of the total weight of the extract. The invention also discloses a preparation method and the quality control method of the effective part extract. The effective part extract contains one effective part group of total lignanoid components and can effectively treat central nervous system degenerative diseases including senile dementia, tristimania and the like. The process for preparing the effective part extracts of the valeriana amurensis P.Smirn. for resisting the senile dementia can adapt to industrial production, and provides a new method for treating the central nervous system degenerative diseases by using the valeriana amurensis P.Smirn..

The present invention relates to a composition and method for preventing and treating cancer and modulating cell proliferation using compositions extracted from pinoresinol-rich Olea europaea Caiazzana olives. The present invention provides contacting one or more cells with a pharmaceutical effective amount of a polyphenolic composition isolated from Olea europaea Caiazzana olives. The polyphenolic composition includes one or more polyphenolic compounds extracted from Olea europaea Caiazzana olives.

Aiming at the phenomena that pinoresinol in the extraction process is easy to decompose to severely affect production of the pinoresinol on a large scale, the method provided by the invention separates and purifies pinoresinol and chlorogenic acid gradually by adopting ethanol extraction, rising film concentration, column chromatography and chromatographic separation, so that the requirements on production of the pinoresinol and chlorogenic acid on a large scale are realized, thereby effectively improving the yield of pinoresinol in eucommia leaves and meanwhile purifying high content chlorogenic acid.

The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

The invention discloses a method of preparing a dilactone type pinoresinol precursor under the existence of cuprous iodide and a molecular sieve through directly promoting a one-step reaction between vanillin and maleate by utilizing metal samarium. The vanillin and the maleate are mixed and dissolved, under stirring, into tetrahydrofuran dehydrated and dried in advance, and then the metal samarium powder, the cuprous iodide powder and the molecular sieve are added into the mixture in order. A reaction system is performed under a condition of absolutely no water. The mixture is stirred at room temperature until the reaction is finished. The obtained reaction mixed solution is subjected to solvent recovery, and a residue is subjected to after-treatment and chromatographic column separation to obtain a high-purity product of the dilactone type pinoresinol precursor.

The invention belongs to the field of medicine, and relates to application of lignan compounds in preparing an anti-hepatitis B virus medicine. According to the invention, the lignan compound 4-keto pinoresinol is extracted and isolated from Helicteres H.angustifolia, and upon tests, the lignan compound 4-keto pinoresinol is proved to have an anti-hepatitis B virus activity; by comparing the compound with lamivudine, which is taken as positive control, through a pharmacological test, a result shows that the compound is significant in anti-hepatitis B virus effect, and IC50 value to HBsAg is 36.6[micron]g / ml and IC50 value to HBeAg is 126.4[micro]g / ml, significantly greater than the positive control, namely the lamivudine; therefore, the compound disclosed by the invention can be used for preparing the anti-hepatitis B virus medicine as an active ingredient.

The invention provides application of pinoresinol in preparation of a medicine for inhibiting thrombosis, and relates to the field of chemical medicines. Specifically provided is the application of a compound shown by formula I, or a salt thereof, or a solvate thereof, or a hydrate thereof, or a prodrug thereof in the preparation of the medicine for inhibiting thrombosis. The compound shown by the formula I is preferably pinoresinol. Researches find that the compound pinoresinol extracted from t. maculata machride can be used for inhibiting vasoconstriction and has a remarkable effect of relaxing blood vessels. Meanwhile, the compound pinoresinol has an inhibiting effect on thrombosis, the thrombosis inhibiting effect of low-dose pinoresinol is better than that of high-dose pinoresinol, the thrombosis inhibiting rate is as high as 76.92%, the excellent thrombosis inhibiting effect is achieved while the medicine using dose is reduced, and the medicine using safety is improved. The compound pinoresinol can be used for preparing the medicine for inhibiting thrombosis, is used for preventing or treating thromboembolic diseases, and has a good application prospect.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a eucommia lignanoid preparation and a preparation method of the eucommia lignanoid preparation. The eucommia lignanoid preparation comprises the following raw materials in percentage by weight: 5%-20% of pinoresinol diglucoside, 5%-15% of syringaresinol diglucoside, 1%-10% of pinoresinol A diglucoside, 5%-15% of olivil biglucoside, and 1%-5% of naringenin B, wherein a eucommia lignanoid extract preferably contains 10%-15% of pinoresinol diglucoside, 8%-12% of syringaresinol diglucoside, 5%-8% of pinoresinol A diglucoside, 8%-12% of olivil biglucoside and 2%-4% of naringenin B.

The invention discloses a rosin furan preservative for dipping cotton wood and a preparation method and an application thereof. The preservative is mainly prepared from the following raw materials in parts by weight: 100 parts of pinoresinol solution and 20 to 30 parts of furan monomer, wherein the pinoresinol solution is prepared in the step of mixing 20 to 30 parts of rosin with 100 to 200 parts of organic solvents in parts by weight. The rosin furan preservative for dipping the cotton wood has the advantages of simplicity in preparation, low cost and no toxic effect; conventional high-pollution heavy-metal chemical preservation can be effectively replaced, the pollution is reduced, a preservative effect is good, and the durability of the cotton wood can be effectively increased; and by dipping in the rosin furan polymer preservative, the physical and chemical properties of the cotton wood can be changed, so that the cotton wood has the advantages of good corrosion resistant performance and good leach resistant performance and does not contain heavy metal, and the mothproof, antifungal and rotproof performance is improved.

Disclosed is an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction for transferring a hydroxy group into a lignan, preferably an enzyme capable of catalyzing the hydroxylation reaction from piperitol into 9-hydroxypiperitol or from pinoresinol into 9-hydroxypinoresinol. Specifically disclosed are: a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the polypeptide; a vector or transformant carrying the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity by using the transformant; and others. The transformant having the polynucleotide introduced therein in an expressible manner is useful for the hydroxylation of a lignan or the production of a product by using lignan hydroxide in the fields of foods and various industries.

The invention discloses a method for simultaneously determining the contents of eight index components in Miao medicine cynanchum auriculatum by a UPLC-ESI-MS (Ultra Performance Liquid Chromatography-ESI-Mass Spectrometry) method. According to the method, the contents of the eight index components in the Miao medicine cynanchum auriculatum are simultaneously determined by establishing the UPLC-ESI-MS method, wherein the eight index components comprise nodatin, pinoresinol, cynanchum bungeanum benzophenone, ferulic acid, gallic acid, scopoletin, syringic acid and chlorogenic acid. According to the method, an ACQUITYUPLCBEHC18 chromatographic column (2.1 mm*100 mm, 1.7 [mu]m) is adopted, a 0.1% formic acid aqueous solution and a 0.1% formic acid acetonitrile solution are adopted as a mobile phase, gradient elution is performed, the flow rate is 0.35 mL / min, the column temperature is 40 DEG C, and an electrospray ion source (ESI) and a multi-reaction ion monitoring (MRM) mode are adopted for detection. The method disclosed by the invention is simple, rapid, high in sensitivity and good in specificity, and can provide a reference basis for quality control of the cynanchum auriculatum medicinal material.

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

The invention belongs to the technical field of medicines and in particular relates to a composition containing pinoresinol and cycloartenyl resin 9-glucoside as well as a preparation method and application of the composition. The composition is prepared from the following components: cycloartenyl resin 9-glucoside, chlorogenic acid, pinoresinol, eucommiol and quercetin. The composition provided by the invention can be used for effectively improving the effect of treating gonitis; meanwhile, the utilization rate of medicines is relatively high, and the cost is effectively reduced; and meanwhile, side effects of the medicines are reduced.

The invention discloses a method for measuring the content of pinoresinol diglucoside in eucommia ulmoides medicinal materials. The method comprises the following steps: Step 1): preparing a referencesolution of pinoresinol diglucoside by using a methanol solution as a solvent; Step 2): processing eucommia ulmoides medicinal materials into floccules to obtain a sample to be detected; Step 3): sequentially extracting the sample to be detected by chloroform and methanol, and dissolving the sample with methanol to obtain a test solution; Step 4): measuring the content of pinoresinol diglucosidein the reference solution and the test solution by high performance liquid chromatography; the chromatographic detection conditions are as follows: octadecylsilane chemically bonded silica is used asa filler; the volume ratio of a mobile phase is 25:75 methanol-water solution; the detection wavelength is 277 nm; the sample size is 10 uL; and the technical scheme adopted by the invention solves the problems that the spectrogram obtained by the eucommia ulmoides detection method has poor chromatographic peak performance and more miscellaneous peaks when the eucommia ulmoides content is determined in the prior art.

The invention discloses applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress. The chemical formula of pinoresinol is shown in the description, for the pinoresinol, by activating the Nrf2 signal channel, the protein levels of the antioxidant enzyme gamma GCS and the II phase detoxification enzyme NQO1 can be up-regulated, the GSH level of the endogenous antioxidant of cells is increased, and the cellular injuries induced by oxidative stress are inhibited; the pinoresinol has the similar cell protection effects for the human bronchusepithelial Beas-2B cells, the human renal glomerular endothelial cells HRGEC cells, the human nerve SHSY5Y cells and the human breast cancer MDA-MB-231 cells. The lignans can be used for preparing health products and medicines for preventing or treating diseases induced by oxidative stress.

The invention provides a throat-smoothing medicine with effects of relieving throat discomfort, soothing throat and relieving stuffy nose. The throat-smoothing medicine comprises the following raw materials in parts by weight: 8 to 12 parts of glucose, 6 to 7 parts of sugarcane, 13 to 37 parts of water, 5 to 8 parts of pigment, 15 to 20 parts of natural mint, 15 to 18 parts of eucalyptol, 8 to 12 parts of thymol and 7 to 10 parts of pinoresinol. Compared with the prior art, the throat-smoothing medicine has the beneficial effects that a plurality of effects of clearing and benefiting throat, clearing heat and detoxicating, eliminating swelling and relieving pain and the like are realized; the therapeutical effect of treating voice hoarseness, acute laryngopharyngitis and the like is good; the main ingredients are herbaceous plant extracts, so that no side effect is generated after use, and the throat-smoothing medicine is suitable for various populations.

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

Aiming at the phenomena that pinoresinol in the extraction process is easy to decompose to severely affect production of the pinoresinol on a large scale, the method provided by the invention separates and purifies pinoresinol and chlorogenic acid gradually by adopting ethanol extraction, rising film concentration, column chromatography and chromatographic separation, so that the requirements on production of the pinoresinol and chlorogenic acid on a large scale are realized, thereby effectively improving the yield of pinoresinol in eucommia leaves and meanwhile purifying high content chlorogenic acid.

The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g / L and the conversion rate is 61.55%.

The invention relates to the field of separation and detection of substances, and provides a method for synchronous detection of coniferyl alcohol and pinoresinol. First, high performance liquid chromatography is used to measure the absorbance of coniferyl alcohol and pinoresinol in a sample to be tested, wherein the detection wavelength is 280nm; The high-performance liquid chromatography adopts gradient elution, and the eluent in the gradient elution includes methanol and water; then according to the standard curve of coniferyl alcohol and the absorbance of coniferyl alcohol in the test sample, the coniferyl alcohol in the test sample is obtained. The concentration of alcohol; According to the standard curve of pinoresinol and the absorbance of pinoresinol in the test sample, obtain the concentration of pinoresinol in the test sample. The method provided by the invention can effectively separate coniferyl alcohol and pinoresinol in the sample to be tested, and detect the corresponding concentrations of coniferyl alcohol and pinoresinol respectively.

The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

The invention relates to a method for enzymatic synthesis of pinoresinol active substances, which includes the steps: mixing water or phosphate buffer solution with water-soluble organic solvent into mixed solution; adding pinoresinol substrate into the mixed solution; adding Rhus Laccase to react at the temperature of 20-45 DEG C for 48-96 hours; after reaction, adding a proper amount of methanol and sodium tartrate into reaction product, subjecting the reaction product to ethyl acetate extraction, anhydrous sodium sulfate drying and concentration, and performing methanol recrystallization to obtain crystal or performing silica-gel column chromatography, concentration and purification to obtain chromatographic product; and subjecting the crystal or chromatographic product to nitrogen blowing or freeze-drying to obtain pinoresinol active substances. The method has moderate conditions, reaction is environment-friendly, simple, quick and low-cost, and the method has broad application prospect in the fields of preparing similar biological medicinal products, active complex compounds, and the like.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a eucommia lignanoid preparation and a preparation method of the eucommia lignanoid preparation. The eucommia lignanoid preparation comprises the following raw materials in percentage by weight: 5%-20% of pinoresinol diglucoside, 5%-15% of syringaresinol diglucoside, 1%-10% of pinoresinol A diglucoside, 5%-15% of olivil biglucoside, and 1%-5% of naringenin B, wherein a eucommia lignanoid extract preferably contains 10%-15% of pinoresinol diglucoside, 8%-12% of syringaresinol diglucoside, 5%-8% of pinoresinol A diglucoside, 8%-12% of olivil biglucoside and 2%-4% of naringenin B.

The invention discloses a solenopsis invicta pheromone attractant and killing agent containing same, and relates to the technical field of pest control. The pheromone attractant is prepared from the following raw materials: 1 to 15 parts of linoleic acid, 1 to 15 parts of linolenic acid, 1 to 15 parts of arachidonic acid, 1 to 20 parts of omega-3 fatty acid, 1 to 15 parts of oleic acid, 1 to 10 parts of acetic acid, 1 to 15 parts of fatty glyceride, 1 to 15 parts of acetate, 1 to 15 parts of mannitol, 1 to 12 parts of sorbitol, 1 to 15 parts of butylene glycol, 1 to 15 parts of pinoresinol, 1 to 15 parts of dihydrocarveol, 1 to 15 parts of terpin, 1 to 50 parts of ethanol, and 1-20 parts of myrtenol. The killing agent further comprises a non-metal oxide and / or at least one of bacteria, fungi and insect protozoon. The solenopsis invicta pheromone attractant has the effects of solenopsis invicta food calling pheromone and solenopsis invicta tracing pheromone at the same time, the feeding amount of solenopsis invicta to the attractant is remarkably increased, the trapping and killing effect of the solenopsis invicta attractant is improved, and meanwhile the solenopsis invicta attractant has extremely high sensitivity and high specificity.

The invention discloses a method for generating pinoresinol and pinoresinol diglucoside through bioconversion of glucose by utilizing phomopsis. The method comprises the following steps: 1) preparing phomopsis into a frozen-dry thallus; 2) adding the frozen-dry thallus into sterilized water containing glucose, and shaking the flask containing the sterilized water for bioconversion reaction; 3) after conversion cultivation, carrying out centrifugation to remove the thallus, conducting vacuum concentration on the converted liquid, adding alcohol for alcohol precipitation, staying over, filtering out polyose and other impurities, and evaporating to obtain the dry pinoresinol diglucoside. According to the method, glucose serves as the only substrate, and pinoresinol and pinoresinol diglucoside can be generated under simple reaction conditions.

The invention relates to the field of substance separation detection and provides a synchronous detection method of coniferyl alcohol and pinoresinol. Absorbance values of coniferyl alcohol and pinoresinol in a to-be-detected sample are measured by using high performance liquid chromatography, wherein the detection wavelength is 280 nm and the high-performance liquid chromatography employs gradient elution and the eluent in the gradient elution includes methanol and water; according to a standard curve of coniferyl alcohol and the absorbance of the coniferyl alcohol in the coniferyl alcohol, the concentration of the coniferyl alcohol in the to-be-detected sample is obtained; and on the basis of the standard curve of pinoresinol and the absorbance of pinoresinol in the to-be-detected sample, the concentration of the pinoresinol in the to-be-detected sample is obtained. Therefore, the coniferyl alcohol and pinoresinol in the to-be-detected sample can be separated effectively; and the corresponding concentrations of the coniferyl alcohol and pinoresinol can be detected respectively.

The invention discloses a method for preparing pinoresinol from salted turnips, which comprises the following steps: 1, the preparation of dry salted-turnip powder: slicing salted turnips, drying at a temperature of 50 DEG C, crushing, sieving by using a 20-mesh sieve so as to obtain dry salted-turnip powder, putting the dry salted-turnip powder into a sealed bag, and putting the sealed bag into a dryer for standby application; 2, the preparation of a salted-turnip crude extract; 3, the preliminary separation of the salted-turnip crude extract; 4, the separation and purification of a component Y1; and 5, the separation and purification of components 1-2: after a moderate amount of alcohol is added into the components 1-2 for dissolving, enabling the obtained object to pass through a reverse-phase silica gel column to carry out separation, eluting by using 50% CH3OH, and selecting a fourth flow component and separating by using a normal phase silica gel; eluting by using petroleum ether, collecting flow components of 97th to 116th pipes, merging the flow components, concentrating the obtained object, and removing a solvent by evaporating, so that pinoresinol is obtained. According to the invention, pinoresinol is separated from salted turnips for the first time, and as a main antifungal ingredient of the salted turnips, the pinoresinol is of great significance for the secondary development of salted turnips.

The invention discloses a method for preparing pinoresinol from salted turnips, which comprises the following steps: 1, the preparation of dry salted-turnip powder: slicing salted turnips, drying at a temperature of 50 DEG C, crushing, sieving by using a 20-mesh sieve so as to obtain dry salted-turnip powder, putting the dry salted-turnip powder into a sealed bag, and putting the sealed bag into a dryer for standby application; 2, the preparation of a salted-turnip crude extract; 3, the preliminary separation of the salted-turnip crude extract; 4, the separation and purification of a component Y1; and 5, the separation and purification of components 1-2: after a moderate amount of alcohol is added into the components 1-2 for dissolving, enabling the obtained object to pass through a reverse-phase silica gel column to carry out separation, eluting by using 50% CH3OH, and selecting a fourth flow component and separating by using a normal phase silica gel; eluting by using petroleum ether, collecting flow components of 97th to 116th pipes, merging the flow components, concentrating the obtained object, and removing a solvent by evaporating, so that pinoresinol is obtained. According to the invention, pinoresinol is separated from salted turnips for the first time, and as a main antifungal ingredient of the salted turnips, the pinoresinol is of great significance for the secondary development of salted turnips.

The invention belongs to analysis and detection of drugs, and particularly relates to a method for simultaneously detecting various effective components in compound lonicera granules with high performance liquid chromatography. The method is used for simultaneously detecting neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, forsythiaside A, forsythin, pinoresinol, baicalin and wogonin in the compound lonicera granules with the high performance liquid chromatography and comprises steps as follows: preparation of a reference substance solution, preparation of a solution of a testing product, determination, result calculation and the like. The method effectively solves the problems that quality evaluation of the compound lonicera granules is mainly about single-component content determination and the like in the prior art, and has the advantages of comprehensively characterizing active pharmaceutical ingredients of the compound lonicera granules and being high in repeatability and good in precision and the like.