36 results about "Pinoresinol" patented technology

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

Aiming at the phenomena that pinoresinol in the extraction process is easy to decompose to severely affect production of the pinoresinol on a large scale, the method provided by the invention separates and purifies pinoresinol and chlorogenic acid gradually by adopting ethanol extraction, rising film concentration, column chromatography and chromatographic separation, so that the requirements on production of the pinoresinol and chlorogenic acid on a large scale are realized, thereby effectively improving the yield of pinoresinol in eucommia leaves and meanwhile purifying high content chlorogenic acid.

The invention relates to a method for detecting whole eucommia capsules, belonging to the technical field of medicine. The present invention adopts the same chromatographic condition to simultaneously measure the content of four kinds of lignan compounds such as pinoresinol diglucoside, pinoresin monoglucoside, syringin and pinoresin in the whole Eucommia capsule, and the operation is simple and fast, and the determination result is accurate and heavy. It has good presentability and can better reflect the product quality of Eucommia ulmoides capsules.

The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

The invention relates to a method for enzymatic synthesis of pinoresinol active substances, which includes the steps: mixing water or phosphate buffer solution with water-soluble organic solvent into mixed solution; adding pinoresinol substrate into the mixed solution; adding Rhus Laccase to react at the temperature of 20-45 DEG C for 48-96 hours; after reaction, adding a proper amount of methanol and sodium tartrate into reaction product, subjecting the reaction product to ethyl acetate extraction, anhydrous sodium sulfate drying and concentration, and performing methanol recrystallization to obtain crystal or performing silica-gel column chromatography, concentration and purification to obtain chromatographic product; and subjecting the crystal or chromatographic product to nitrogen blowing or freeze-drying to obtain pinoresinol active substances. The method has moderate conditions, reaction is environment-friendly, simple, quick and low-cost, and the method has broad application prospect in the fields of preparing similar biological medicinal products, active complex compounds, and the like.